Method for preparing oocyte paraffin cut film
A technology of oocytes and slices, which is applied in the preparation of test samples, material inspection products, biological tests, etc., can solve the problems of antibody consumption, inhomogeneity, and oocyte structure damage, and achieve the reduction of antibody usage, Less non-specific staining and clear detection signal
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[0027] The preparation of embodiment 1 oocyte slice
[0028] In this example, avian oocytes were used as samples to prepare paraffin sections of ovarian cells, which specifically included the following steps:
[0029] 1. Obtaining and fixing oocytes: select hens with good laying records to be sacrificed to obtain ovaries, and oocytes are stripped and cultured in vitro. Oocytes cultured in vitro for different periods of time are sucked out with a glass pipette under an anatomical microscope. Fix overnight in a glass bottle containing 10% neutral formaldehyde;
[0030] 2. Dehydration and transparent treatment of oocytes: take 4 glass bottles, add 75%, 85%, 95%, 100% alcohol, about 2 / 3 volume. Use a pipette gun to suck out the oocytes in the formaldehyde fixative, and dehydrate them step by step from 75% to 100% alcohol for 1 hour each. Suck the dehydrated oocytes into a small foot measuring cup (10ml) with a pipette, inject 5ml of xylene to make it transparent for 1 hour, and ...
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