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Rotating type diffused fluorescent chromatographic imaging system

A fluorescence tomography and imaging system technology, applied in the field of biofluorescence tomography, can solve the problems of limited imaging quality and limited data volume, and achieve the effect of stable and effective data collection

Inactive Publication Date: 2009-09-02
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Continuous light source irradiation and camera data collection are also used, but since the light source and camera are fixed, the amount of collected data is limited, and the imaging quality is limited by the amount of collected data

Method used

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  • Rotating type diffused fluorescent chromatographic imaging system
  • Rotating type diffused fluorescent chromatographic imaging system
  • Rotating type diffused fluorescent chromatographic imaging system

Examples

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example 1

[0039] The parameters of semiconductor laser 1 are: 652nm / 50mW, spot diameter 0.38mm, 180° up and down offset 0.355mm. The imaging sample 10 is a cylindrical glass tube with an outer diameter of 25.4 mm and a wall thickness of 1 mm. The glass tube is filled with 1% Intrilipid aqueous solution and two small transparent plastic tubes. Both plastic tubes were filled with 2.0% Intrilipid aqueous solution, and two drops of ink were added in one plastic tube 17 (diameter 4 mm), and one drop of ink was added in the other plastic tube 18 (diameter 3.6 mm). Two plastic pipes 17, 18 are parallel, and the central axes of the two pipes are 8.6mm apart. The structural schematic diagram of this imaging sample 10 is shown in Figure 5a Three-dimensional schematic diagram of glass tube imaging sample, Figure 5b Schematic diagram of the cross-section of the imaging sample in the glass tube. Utilize the system of the present invention to collect data, calculate and obtain the absorption coe...

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Abstract

The invention discloses a rotary diffusion fluorescence tomography imaging system, which comprises a semiconductor laser, a computer, a rotary worktable, a stepping motor and a CCD camera. The semiconductor laser and the semiconductor refrigeration box are respectively fixed on the two horizontal arms of the rotary table, and the CCD camera is located in the semiconductor refrigeration box; the sample fixing clip is fixed on the bracket, and is located between the CCD camera and the semiconductor laser; the stepping motor drives the CCD The camera and semiconductor laser rotate around the center point of the vertical rod of the rotary table; the computer sends control signals to the rotary table and the CCD camera, receives the image data sent by the CCD camera, and analyzes and processes the image data. The system of the invention can freely control the size of the collected data and improve the imaging effect; it can realize the cooperative work of the rotation of the stepping motor and the shooting control of the cooling charge-coupled camera. In this way, the cooled charge-coupled camera can collect data stably and effectively during the rotation of the system.

Description

technical field [0001] The invention belongs to biological fluorescence tomography technology, and relates to a rotary fluorescence tomography technology (fluorescence diffuse optical tomography, FDOT) based on near-infrared light diffusion properties, specifically a rotary diffusion fluorescence tomography system, which is suitable for In vivo non-invasive detection of carcinogenesis in small animals. technical background [0002] Using the diffusion properties of near-infrared light, the in vivo non-invasive detection technology of small animals is a promising optical imaging technology. Compared with microscopic imaging and optical weak coherence tomography technology, it has the highest imaging depth The deepest optical imaging method. There is a US Patent No. 7.107.116 applied for abroad. The technical means used in this invention is to squeeze the small animals into a flat shape, and use the diffusion equation in the semi-infinite medium to derive the optical paramete...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B5/00A61B10/00G06F17/11
Inventor 骆清铭曾绍群毕昆全国涛
Owner HUAZHONG UNIV OF SCI & TECH
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