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Bifidobacteria viable bacteria preparation and special-purpose protective agent thereof

A technology of bifidobacteria and live bacteria preparations, applied in the direction of bacteria, etc., can solve the problems of unsolved storage at room temperature, easy inactivation, etc., and achieve the effects of solving the difficulty of storage at room temperature, easy to operate, and simple method.

Active Publication Date: 2009-07-29
LIVZON PHARM GRP INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Strains are easily inactivated during production, transportation and storage
In order to keep the number of viable bacteria in the product at a certain level, researchers at home and abroad have done a lot of research on bacterial genetic modification, microencapsulation technology, and bacterial cell embedding technology. However, from the current point of view, the product is often used It is required to be stored at low temperature, and most products containing bifidobacteria have not solved the problem of room temperature storage

Method used

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  • Bifidobacteria viable bacteria preparation and special-purpose protective agent thereof
  • Bifidobacteria viable bacteria preparation and special-purpose protective agent thereof
  • Bifidobacteria viable bacteria preparation and special-purpose protective agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the preparation of bifidobacteria viable bacteria preparation and its storage time test

[0022] 1. Preparation of live bifidobacteria preparations

[0023] 1. Activation of bifidobacteria

[0024] The strain Bifidobacterium adolescentis DM8504 (purchased from Dalian Medical College) was inoculated on the modified BS agar slant medium. Then put it into an anaerobic incubator, and cultivate it anaerobically for 48-72hrs at a temperature of 37±0.5°C.

[0025] Components of modified BS medium: tomato juice 200ml, soybean peptone 15g, yeast extract 6g, glucose 10g, Tween 80 1g, tryptone 10g, beef extract 0.3g, soluble starch 0.5g, beef liver extract 5ml, complex salt 4.0 ml of solution, 0.5 g of L-cysteine ​​hydrochloride, 15 g of agar (used when preparing solid medium). The composition of the complex salt solution is: K 2 HPO 4 1.0g, CaCl 2 0.2g, MgSO 4 ·7H 2 O 0.48g, KH 2 PO 4 1.0g, NaHCO 3 10.0 g, NaCl 2.0 g.

[0026]The above-mentioned impr...

Embodiment 2

[0071] Embodiment 2, the preparation of bifidobacteria live bacteria preparation and its storage time test

[0072] 1. Preparation of live bifidobacteria preparations

[0073] 1. Activation of bifidobacteria

[0074] The strain Bifidobacterium infantis ATCC 15697 (purchased from the American ATCC Culture Collection) was inoculated on the modified TPY medium slant medium. Then put it into an anaerobic incubator, and cultivate it anaerobically for 48-72hrs at a temperature of 37±0.5°C.

[0075] The above-mentioned improved TPY medium is made from the following components: tryptone 12g / L, Peptone 3g / L, Soy Peptone 5g / L, Glucose 5g / L, Maltooligosaccharide 5g / L, Yeast Extract Powder 5g / L, Tween-801.0g / L, L-Cysteine ​​Hydrochloride 0.5g / L, beef liver infusion 200mL / L, tomato juice 100mL / L, mixed salt solution 5mL / L, distilled water 675mL / L, adjust pH to 7.0-7.2, 121 ℃, sterilize for 15-20 minutes.

[0076] Mixed salt solution: K 2 HPO 4 20.0g, MgCl 2 ·6H 2 O 5.0g, ZnSO 4...

Embodiment 3

[0112] Embodiment 3, the preparation of bifidobacteria viable bacteria preparation and its storage time test

[0113] 1. Preparation of live bifidobacteria preparations

[0114] 1. Activation of bifidobacteria

[0115] The strain Bifidobacterium adolescentis CGMCC 1.2190 (purchased from China General Microorganism Culture Collection and Management Center) was inoculated on the modified BS agar slant medium. Then put it into an anaerobic incubator, and cultivate it anaerobically for 48-72hrs at a temperature of 37±0.5°C.

[0116] Components of modified BS medium: tomato juice 200ml, soybean peptone 15g, yeast extract 6g, glucose 10g, Tween 80 1g, tryptone 10g, beef extract 0.3g, soluble starch 0.5g, beef liver extract 5ml, complex salt 4.0 ml of solution, 0.5 g of L-cysteine ​​hydrochloride, 15 g of agar (used when preparing solid medium). The composition of the complex salt solution is: K 2 HPO 4 1.0g, CaCl 2 0.2g, MgSO 4 ·7H 2 O 0.48g, KH 2 PO 4 1.0g, NaHCO 3 1...

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Abstract

The invention discloses a preparation method of bifidobacteria living bacteria preparation and a special protective agent thereof. The protective agent is composed of the following components by weight: 0.01-5.0 parts of glycerin; 0.1-6.0 parts of polyvinylpyrrolidone; 0.1-5.0 parts of polyethylene glycol; 1.0-10.0 parts of skimmed milk powder; 0.5-15.0 parts of trehalose ; 0.1-5.0 parts of sodium glutamate, 0.1-5.0 parts of L-aspartic acid; 0.1-5.0 parts of sodium vitamin C; 0.1-5.0 parts of ornithine hydrochloride. The method of the invention solves the problem that the bifidobacterium products produced by conventional methods are difficult to preserve at room temperature.

Description

technical field [0001] The invention relates to a bifidobacteria viable bacteria preparation and a special protective agent thereof. Background technique [0002] Bifidobacterium (Lactobacillus bifidus) is a class of Gram-positive anaerobic bacteria, which is one of the most abundant and common normal flora in the intestinal tract of healthy people. It was first isolated from the feces of healthy breastfed infants by Dr. Tissier of the Pasteur Institute in France in 1899. After more than 30 years of research, researchers mainly in Japan and various countries including European countries gradually discovered the beneficial effects of bifidobacteria on the human body, and it has become a function of measuring body health and evaluating and improving gastrointestinal flora. One of the symbols of sexual food. Bifidobacterium is the dominant bacteria in the gastrointestinal tract of healthy adults and long-lived elderly. It changes dynamically with age and is closely related to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 苏日佳胡振湘徐乐意王云华王燕清
Owner LIVZON PHARM GRP INC
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