Selenium-rich and chromium-rich Saccharomyces cerevisiae, selenium-rich and chromium-rich yeast product and their production process

A technology of Saccharomyces cerevisiae and chromium yeast, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as high production costs and complex processes

Active Publication Date: 2009-01-14
ANGEL NUTRITECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pure selenium-enriched yeast and chromium-enriched yeast need to be produced separately, the process is more complicated, and correspondingly higher production costs are required

Method used

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  • Selenium-rich and chromium-rich Saccharomyces cerevisiae, selenium-rich and chromium-rich yeast product and their production process
  • Selenium-rich and chromium-rich Saccharomyces cerevisiae, selenium-rich and chromium-rich yeast product and their production process
  • Selenium-rich and chromium-rich Saccharomyces cerevisiae, selenium-rich and chromium-rich yeast product and their production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Obtaining yeast strains

[0015] The selenium-enriched chromium Saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen Z1.1) used in the present invention has been biologically preserved in the China Center for Type Culture Collection (CCTCC, Wuhan University, zip code 430072) on October 25, 2005, and the deposit number is: CCTCC M 205123. It is composed of selenium-enriched yeast strain S205 (see the patent application titled "a selenium-enriched Saccharomyces cerevisiae, selenium-enriched yeast product and its production method" filed by the applicant at the same time as the present application) and chromium-enriched yeast strain C460 ( Please refer to the patent application titled "A Chromium-enriched Saccharomyces cerevisiae, Chromium-enriched Yeast Product and Its Production Method" filed at the same time as this application), which was selected and bred by protoplast fusion technology. The specific implementation steps are as follows:

[0016] 1) S...

Embodiment 2

[0021] Example 2: Production of selenium-enriched chromium yeast product

[0022] The production process of selenium-enriched chromium yeast: slant culture → primary liquid seed culture → secondary liquid seed culture → fermentor seed culture → fermenter culture → separation → drying → packaging. The specific process is as follows:

[0023] 1) Slant culture: inoculate selenium-enriched chromium yeast strains to a sugar content of 5% by weight, selenium (selenite) concentration of 1 μg / ml, chromium (chromium trichloride) 1 μg / ml, pH The wort solids slant with a value of 4 was incubated at 26°C for 48 hours.

[0024] 2) First-level liquid seed culture: inoculate 1 to 6 rings of yeast strains on the inclined plane to 500 ml of sugar content of 5% by weight, selenium (selenite) concentration of 1 μg / ml, chromium (trichloride) Chromium) 1 μg / ml, pH 4 special culture flask for wort, cultured at 26°C for 48 hours.

[0025] 3) Secondary liquid seed culture; the primary liquid seed ...

Embodiment 3

[0031] Example 3: Production of selenium-enriched chromium yeast product

[0032] The production process of selenium-enriched chromium yeast is with embodiment 2, and concrete technology is as follows:

[0033] 1) Slant strain culture: Inoculate selenium-enriched chromium yeast strains to 25% sugar, 1000 μg / ml selenium (selenate) concentration, 1000 μg / ml chromium (chromium acetate), and pH 6.5. The wort solid slant was incubated at 36°C for 18 hours.

[0034] 2) First-level liquid seed culture: inoculate 1 to 6 rings of yeast strains on the inclined plane to 500 ml of sugar content of 25% by weight, selenium (selenate) concentration of 1000 μg / ml, and chromium (chromium acetate) 1000μg / ml, pH 6.5 special culture bottle for wort, cultured at 36 ℃ for 18 hours.

[0035] 3) secondary liquid seed culture; inoculate the first-level liquid seed culture liquid to containing 1000 liters of sugar (molasses) in an amount of 25% by weight, selenium (selenate) concentration of 1000 μg / ...

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Abstract

The present invention relates to one kind of selenium-rich and chromium-rich Saccharomyces cerevisiae, selenium-rich and chromium-rich Saccharomyces cerevisiae product and their production process. Of the selenium-rich and chromium-rich Saccharomyces cerevisiae product, each kilogram contains selenium in 100-4000 mg and chromium in 100-4000 mg. The selenium-rich and chromium-rich Saccharomyces cerevisiae may be used as selenium and chromium replenisher added into various food products or prepared into alone preparation. The selenium-irch and chromium-rich Saccharomyces cerevisiae product has lower toxicity than inorganic selenium and chromium product and high safety, and may be used in replenishing both selenium and chromium.

Description

technical field [0001] The invention relates to a selenium-enriched and chromium-enriched Saccharomyces cerevisiae, a selenium-enriched and chromium-enriched yeast product obtained from the selenium-enriched and chromium Saccharomyces cerevisiae, and a production method thereof. Background technique [0002] Selenium and chromium are essential trace elements for the human body, which have important physiological functions for the human body, especially in maintaining blood sugar, blood lipids and blood pressure in the body and anti-cancer. Inorganic selenium and chromium are more toxic to human body, and yeast can absorb and utilize inorganic selenium and chromium in the medium during growth, and convert inorganic selenium and chromium into components in yeast cells. Converting inorganic selenium and chromium into yeast selenium and yeast chromium can greatly reduce toxicity. There are many studies producing selenium and chromium separately. However, the simple selenium-en...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12R1/865
Inventor 俞学锋李知洪余明华姚娟张彦匡金宝朱娅敏马灿许武桥夏长虹
Owner ANGEL NUTRITECH CO LTD
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