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Method for separating and purifying natural product using three-phase counter current chromatograph

A technology of countercurrent chromatography and natural products, which is applied in the field of separation and purification of natural products using three-phase countercurrent chromatography, which can solve the problems of low overall resolution and inability to realize compounds, and achieve the effect of solving the problem of low overall resolution

Active Publication Date: 2008-11-19
四川广铭生物制品有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The purpose of the present invention is to overcome the low overall resolution when using countercurrent chromatography to separate natural products, and when using column chromatography to separate natural products, due to the limitation of selectivity, some structurally similar compounds cannot be realized by one-step method, especially It is a defect in the effective separation of highly hydrophilic compounds, thereby providing a method for the separation and purification of natural products using three-phase countercurrent chromatography that combines the solid separation medium of the countercurrent chromatography method and the column chromatography method - microspheres.

Method used

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  • Method for separating and purifying natural product using three-phase counter current chromatograph

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Embodiment 1

[0037] Embodiment 1, separation and purification of Scutellaria baicalensis water extract

[0038] Use agarose matrix gel Superose 12 and n-hexane-ethyl acetate-methanol-acetic acid-water (volume ratio is 1-6-1.5-1.5-6) ​​system to constitute the three phases of the three-phase countercurrent chromatography system of the present invention, to Separating and purifying the water extract of Scutellaria baicalensis, the specific steps are:

[0039] 1) According to the volume ratio of 1:6:1.5:1.5:6, mix n-hexane, ethyl acetate, methanol, acetic acid and water, and balance in a separatory funnel for 2 hours, the mixed solution is divided into upper and lower phases, and n-hexane Alkane-ethyl acetate-methanol-acetic acid-water solvent system; the upper and lower phases are separated;

[0040] 2) Use Superose 12 gel (GE Healthcare, average particle diameter 10 μm) as the solid stationary phase; the lower phase in the solvent system in step 1) whose polarity is similar to the solid st...

Embodiment 2

[0045] Embodiment 2, the separation and purification of Danshen fat-soluble compound

[0046] Use reverse-phase bonded phase silica gel C18 and n-hexane-ethanol-water (volume ratio is 10-5.5-4.5) system to constitute the three phases of the three-phase countercurrent chromatography system of the present invention to separate and purify the fat-soluble compounds of Salvia miltiorrhiza, and the specific steps are as follows: :

[0047] 1) According to the volume ratio of 10-5.5-4.5, mix n-hexane, ethanol and water, and balance in a separatory funnel for 2 hours, the mixed solution is divided into upper and lower phases, and obtain n-hexane-ethanol-water solvent system; phase separation;

[0048] 2) Use reverse-phase bonded phase silica gel C18 (carbon 18) (GE Healthcare, particle average diameter 5 μm) as the solid stationary phase; use the upper phase in the solvent system in step 1) whose polarity is similar to that of the solid stationary phase as the liquid stationary phase...

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Abstract

The present invention relates to a method for separating and purifying natural product by using three-phase counter-current chromatography. Sad method is characterized by that in the immobile phase of conventional counter-current chromatography a solid separation medium-microsphere can be added so as to form a three-phase separation system including liquid immobile phase, solid immobile phase and liquid mobile phase, so that the counter-current chromatography and column chromatography can be combined into one body, therefore the separation and purification efficiency of natural product can be greatly raised.

Description

technical field [0001] The invention relates to a method for separating and purifying natural products using three-phase countercurrent chromatography. Background technique [0002] In recent years, research on active ingredients in natural products (including traditional Chinese medicine) - natural plants, animals and minerals has become a hot spot in pharmaceuticals, pharmacology and clinical applications. 25% of the world's prescription drugs come from natural plants, of which 121 are active The compound can be used as a routine drug, which has attracted the attention of countries all over the world. [0003] my country has a vast territory and is one of the countries with the richest plant resources in the world. There are more than 30,000 species of higher plants alone, including more than 11,000 species of medicinal plants, with a history of thousands of years of clinical application. However, the coverage rate of Chinese traditional medicine in the international mark...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/08
Inventor 顾铭杨克里斯特杨森欧阳藩
Owner 四川广铭生物制品有限公司
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