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Application of Merremia boisiana extract in preparing bactericide

A technology of extract and fenugreek, applied in the field of pesticides, can solve problems such as unsatisfactory herbicide effect, no obvious effect, harm of fenugreek, and achieve the effects of unique bactericidal action mechanism, high bactericidal activity and simple extraction method

Inactive Publication Date: 2008-11-12
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is extremely difficult to prevent and control the harm of Admirallum vine
The effects of artificial control and the use of herbicides are unsatisfactory: as long as the cut off canes are in contact with the ground, adventitious roots and sprouts will grow quickly; spraying with various herbicides such as moricon and methetengcarb, only It can kill some young stems and leaves, but has no obvious effect on other rhizomes

Method used

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  • Application of Merremia boisiana extract in preparing bactericide
  • Application of Merremia boisiana extract in preparing bactericide
  • Application of Merremia boisiana extract in preparing bactericide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The twig plants are collected separately from old vines (including cortex) and green young vines (including leaves), dried in the shade (or dried at 50 degrees Celsius), mechanically crushed, and sieved with 40-60 mesh. The pulverized matter is cold soaked and extracted 3 times with 5 times the amount of organic solvent methanol at room temperature, and the soaking time is 3 days, 2 days, and 1 day respectively, and the filtrates are collected by filtration sequentially, combined for 3 times. Concentrate under reduced pressure at 55°C, remove methanol, and obtain the dry matter of the methanol crude extract of Admiria aurinae, which is stored in cold storage and used for later use.

Embodiment 2

[0028] The methanol crude extract of the vine (cortex) obtained in Test Example 1 was dissolved with 5 times the amount of 80% ethanol, and the gained solution was placed in a separatory funnel, and at room temperature, the same volume of organic solvent petroleum was added. Extract with ether, first release the ethanol solution in the lower layer, and then release the petroleum ether solution in the upper layer. The petroleum ether solution is concentrated under reduced pressure at a temperature lower than 55 degrees Celsius. After the petroleum ether is recovered, the same volume of petroleum ether is added to the ethanol solution for extraction, and the petroleum ether is concentrated and recovered until the color of the petroleum ether extract is until it becomes colorless and transparent. After the petroleum ether solution is concentrated, the petroleum ether extract can be obtained, which is stored in cold storage and used for later use.

[0029]The remaining ethanol so...

Embodiment 3

[0032] Weigh 1 g of the methanol crude extract of Admiria aurinae in Example 1, add water to dissolve it and make it volume up to 100 ml, and add 100 μl of Tween 20 to prepare a 1% aqueous solution of the extract of Auranthus aurantiae in methanol. In the same way, weigh 1 gram of the extract of Thuja chinensis n-butanol in Example 2, add water to dissolve it and make the volume to 100 ml, and add 100 μl Tween 20 to prepare a 1% Tween twig n-butanol extract aqueous solution .

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Abstract

The present invention provides application of Merremia boisiana extract in preparing bactericide. Merremia boisiana extract has broad-spectrum and high bacteriostasis activity on plant pathogenic fungi, food mildew and plant pathogenic bacteria, and high preventing and treating effect on sheath and cullm blight of rice and frosty mildew of cucumber. The extract is safe to crops and easy to biodegrade, and has no residue and no pollution. In addition, it has action mechanism on plant pathogenic fungi, food mildew and plant pathogenic bacteria dissimilar to common bactericides and thus less resistance. The present invention provides one new kind of bactericide while paving one way of controlling Merremia boisiana as one harmful forest plant.

Description

technical field [0001] The invention belongs to the field of pesticides, and in particular relates to the application of the extract of Thuja chinensis in the preparation of fungicides, and at the same time relates to the preparation method of the plant extract of Thuja japonicus. Background technique [0002] As we all know, pesticides are one of the safeguards for plant disease control. Even today, although the use of disease-resistant genes has been rapidly developed under the impetus of molecular biology-related technologies, chemical pesticides still play an indispensable role in plant disease control. Replacement effect. The advent of the fungicide Thiram in 1934 opened the era of organic synthetic fungicides. Since then, the control of plant diseases has become more efficient, and chemically synthesized fungicides have made positive contributions in the control of plant diseases. However, since the 1960s, the public has paid more and more attention to the toxicity o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/00A01P3/00A01N65/08
Inventor 潘汝谦徐汉虹黄桂荣张慧徐大高
Owner SOUTH CHINA AGRI UNIV
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