Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nanosecond pulsed electric field parameters for destroying tumors with a single treatment

a pulsed electric field and tumor technology, applied in the field of nanosecond pulsed electric field treatment methods and devices, can solve the problem that the field may not pass into the tumor effectively

Active Publication Date: 2013-08-20
PULSE BIOSCI INC
View PDF1 Cites 31 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent text discusses a method of treating tumors using a specialized device that delivers nanosecond pulsed electric fields (nsPEF) to the tissue. The device has a cavity that draws the tissue into it and positions the electrodes to treat the tumor. The treatment involves applying a specific number of pulses at a certain frequency to deliver electrotherapy. By controlling these parameters, such as the number of pulses, frequency, and pulse duration, the method can effectively treat tumors and trigger their apoptosis (a type of cell death) without causing damage to surrounding tissues. The therapy may be applied with a minimum number of pulses, such as 600 or more, depending on the size of the tumor. The method may also involve increasing the applied amplitude and duration of the pulses for optimal results. Overall, the patent text provides a technical solution for delivering nsPEF therapy to tumors and provides guidance on the optimal treatment parameters for triggering tumor apoptosis.

Problems solved by technology

When nsPEF is applied to a tissue region such as a tumor, if a large resistance between the electrode and the tumor restricts current flow (such as the presence of the stratum corneum in skin), the field may not pass into the tumor effectively.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nanosecond pulsed electric field parameters for destroying tumors with a single treatment
  • Nanosecond pulsed electric field parameters for destroying tumors with a single treatment
  • Nanosecond pulsed electric field parameters for destroying tumors with a single treatment

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0071]In optimizing the device, multiple experiments have shown that tumors, such as melanoma tumors, may be eliminated utilizing nsPEF when exposed to 100 ns long pulses having a 15 ns rise time where the minimum number of pulses range from, e.g., 1500 to 2000 pulses, as illustrated in the chart of FIG. 10 which shows the Optimum Pulse Number where a majority of tumors were successfully treated after a single treatment when pulsed with at least 1500 to 2000 pulses. Accordingly, as shown in the graph of FIG. 11, the Percent Efficacy after one treatment is shown to increase from 1500 pulses and higher.

[0072]As also indicated in the chart of FIG. 12 which illustrates Optimum Amplitude of the pulses, the number of tumors successfully treated after a single treatment begins to rise at higher amplitudes, e.g., from 25 kV / cm. Thus, the minimum pulse amplitude observed is 30 kV / cm in this example while the optimum pulse amplitude is 40 kV / cm or greater in this example for effectively treat...

example 2

[0074]In this particular example, Murine B16-F10 melanoma cells transfected with enhanced green fluorescent protein (eGFP) were obtained and stored in liquid nitrogen until use. These cells were cultured and injected into 4-6 week old female Nu / Nu mice (immunodeficient, hairless, albino) using standard procedures at four injection sites each. Tumors were detected visually by the bulges they produced and by GFP detection under fluorescent microscopy.

[0075]Various suction electrode assemblies, shown in FIG. 13, were used where electrode assemblies 110, 112, and 114 each had a recessed cavity with an inner diameter of about 4 mm and a depth of about 2 mm while the electrode assembly 116 utilized an array of needles positioned within the recessed cavity where a distance between the center needle and each of the outer needles was about 2 mm. In each of the assemblies, one or more electrodes 118 were used to discharge the energy into the treated tissue while the remaining electrodes funct...

example 3

[0082]Typical melanoma responses to nsPEF therapy in the 10-25 kV / cm range were recorded where four melanomas on one mouse were treated with either 10, 15, 20 or 25 kV / cm nsPEF (2000 pulses, 100 ns, 7 Hz). The GFP fluorescence at each respective pulse amplitude over a period of 0, 1, 6, and 8 days were recorded, as shown in FIG. 21, as were the trans-illumination, as shown in FIG. 22, and reflected light images, as shown in FIG. 23. The temperature increase inside a tumor over a period of several minutes during nsPEF application were also recorded, as shown in FIG. 24.

[0083]A pulse amplitude of 30 kV / cm with 100 ns long pulses were applied beginning at the indicated frequency 120 with frequency of 1 Hz and 5 Hz. Pulsing was stopped at the indicated frequency 122 for 5 Hz and at 124 for 1 Hz. The 1 Hz pulse application increased tumor temperature by 2° C. while the 5 Hz pulse application increased the temperature by 7° C.

[0084]The appearance of nsPEF-treated skin on the indicated day...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Nanosecond pulsed electric field (nsPEF) parameters for destroying tumors with a single treatment are described. A nsPEF generator may be used with an electrode assembly to apply the pulses to one or more tumors where the parameters for the nsPEF are optimized for treating such tumors.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of priority to U.S. Prov. 61 / 186,798 filed Jun. 12, 2009, which is incorporated herein by reference in its entirety.FIELD OF THE INVENTION[0002]The present invention relates to methods and apparatus for treating tumors utilizing nanosecond pulsed electric fields (nsPEF). More particularly, the present invention relates to methods and apparatus for destroying tumors with nanosecond pulsed electric fields (nsPEF) which are optimized for treating such tumors.BACKGROUND OF THE INVENTION[0003]As described in U.S. Pat. No. 6,326,177 (Schoenbach et al.), which is incorporated herein by reference in its entirety, a method for intracellular electro-manipulation is described. The method includes applying at least one ultrashort electric field pulse to target cells where the ultrashort electric field pulse has sufficient amplitude and duration to modify subcellular structures in the target cells and does not excee...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(United States)
IPC IPC(8): A61B18/18
CPCA61B18/14A61B18/1206A61B18/1477A61B2018/00291A61B2018/00452A61B2018/00577A61B2018/00726A61B2018/126A61B2018/1425A61B2018/143A61B2018/1467
Inventor NUCCITELLI, RICHARD LEENUCCITELLI, PAMELASHEIKH, SALEHTRAN, KEVINATHOS, BRIANKREIS, MARK
Owner PULSE BIOSCI INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com