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Evaluation method and screening method for substance having action of activating/suppressing innate immunity, agent and food product for activating/suppressing innate immune mechanism and method for producing the same

a screening method and substance technology, applied in the direction of dsdna viruses, immunological disorders, instruments, etc., can solve the problems of destroying infectious sources, difficult to inject a certain amount of test liquid into body fluids or intestines, and short body length

Active Publication Date: 2012-11-20
IMAGINE GLOBAL CARE CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This method allows for simple and efficient screening of substances that activate or suppress the innate immune mechanism, excluding pharmacokinetic issues and false positives, and has been shown to detect active compounds in natural products like turmeric with therapeutic potential.

Problems solved by technology

NO rapidly reacts with oxygen and superoxide, and various chemicals produced during its process destroy the infectious source.
However, in such a conventional method, equipments for culturing the cells are required, and there has been a problem that a substance that has problems for pharamaco-kinetics in an individual when administered to the individual is also detected.
Also, lipopolysaccharide (LPS) derived from bacteria and contaminated in the material to be tested reacts at low concentration, thus many materials to be tested exhibit false positive, and this has been an obstacle in the search of a novel substance.
In recent years, the method of screening the substance that acts upon the innate immune mechanism utilizing transgenic Drosophila has been proposed (e.g., see Patent Document 1), but a body length is very short, and thus it is difficult to inject a certain amount of a test liquid into a body fluid or an intestine, which is possible in silkworm larvae.
It is also difficult to isolate an organ to subject to a pharmacological experiment, which is also possible in the silkworm larvae.
Further, in this method, there is also a problem that only a mechanism to which a particular antibacterial peptide is related can be detected because a reporter gene is introduced downstream of an antibacterial peptide gene.

Method used

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  • Evaluation method and screening method for substance having action of activating/suppressing innate immunity, agent and food product for activating/suppressing innate immune mechanism and method for producing the same
  • Evaluation method and screening method for substance having action of activating/suppressing innate immunity, agent and food product for activating/suppressing innate immune mechanism and method for producing the same
  • Evaluation method and screening method for substance having action of activating/suppressing innate immunity, agent and food product for activating/suppressing innate immune mechanism and method for producing the same

Examples

Experimental program
Comparison scheme
Effect test

example 1

Muscular Contraction of Silkworm Larva by Peptideglycan or β-Glucan

[0153]It has been already known that peptideglycan derived from bacteria and β-glucan derived from mushrooms have the action of activating the innate immune system (innate immune mechanism) (e.g., see Adachi & Ohno, “Recognition of fungal polysaccharide by immune system and its activation effect”, Jpn. J. Med. Mycol., 47, 185-194, 2006 and Akira, S., Uematsu, S., and Takeuchi O., Pathogen recognition and innate immunity., Cell, 124, 783-801, 2006). In this Example, the effect of these innate immunity activation substances on the muscular contraction in the silkworm larva was examined.

[0154]Peptideglycan derived from Staphylococcus aureus (Sigma) or β-glucan derived from the mushroom (an extract from Agaricus mushroom with hot water was used in this Example) was suspended in 0.9% NaCl, then 0.05 mL thereof was injected in a body cavity of an isolated head muscle specimen of the silkworm larva (see Sekimizu et al., J. ...

example 2

Muscular Contraction by Silkworm (Bombyx mori) Paralytic Peptide BmPP in Silkworm Larvae

[0161]It has been known that when a body fluid is removed from a silkworm larva and the body fluid is further injected into the silkworm larva again, the silkworm larva is paralyzed. This paralytic substance is a peptide referred to as BmPP composed of 24 amino acid residues (see Ha et al., Peptides, 20, 561-568, 1999). In this Example, a synthetic product of this peptide was injected into the isolated head muscle specimen of the silkworm larvae, and its reaction was examined.

[0162]BmPP (provided by Dr. Manabu Kamimura, National Institute Agrobiological Sciences) at various concentrations were dissolved in 0.9% NaCl, 0.05 mL thereof was injected in the body cavity of the isolated head muscle specimen of the silkworm larva, the body length was measured after 6 minutes, and the C value was calculated in the same manner as in Example 1.

[0163]Also, 0.05 mL of the solution in which L-glutamic acid (40...

example 3

Inhibition of Peptideglycan-Induced Muscular Contraction by Anti-BmPP Antibody

[0167]The muscular contraction induced by BmPP is inhibited by the anti-BmPP antibody. It was examined whether the muscular contraction induced by peptideglycan was inhibited by the anti-BmPP antibody or not.

[0168]After injecting 0.05 mL of rabbit antiserum against BmPP into the body cavity of the isolated head muscle specimen of the silkworm larva, immediately 0.05 mL of 0.4 μg / mL of the BmPP solution or the peptideglycan (in this Example, autoclaved Staphylococcus aureus was used) suspension was injected, and the body length when the maximum contraction was exhibited (after about 10 minutes) was measured and the C value was calculated in the same manner as in Example 1. As the control experiment, 0.9% NaCl in place of the rabbit antiserum against BmPP was injected.

[0169]The results are shown in FIG. 5.

[0170]It was found that the muscular contraction induced by peptideglycan in the silkworm larva was inhi...

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Abstract

It is intended to provide an evaluation method and a screening method capable of eliminating a substance that disturbs in vivo kinetics in an individual and capable of simply and easily searching a substance having an action of activating / suppressing an innate immune mechanism without being affected by LPS derived from bacteria, which can be contaminated during the search, as well as a drug and a food for activating / suppressing the innate immune mechanism, and methods of producing the same. The present invention provides a method of evaluating or screening the substance having the action of activating / suppressing the innate immune mechanism using a muscular contraction of an organism having the innate immune mechanism as an indicator, and methods of producing the drug and the food for activating / suppressing the innate immune mechanism. Also, an innate immunity activator and the food having the action of activating the innate immune mechanism containing the substance having the action of contracting the muscle of the organism having the innate immune mechanism, and an innate immunity suppressor and the food having the action of suppressing the innate immunity containing the substance having the action of suppressing the contraction of the muscle of the organism having the innate immune mechanism are provided.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a National Stage of PCT / JP08 / 057,105 filed Apr. 10, 2008 and claims the benefit of JP 2007-102918 filed Apr. 10, 2007.TECHNICAL FIELD[0002]The present invention relates to an evaluation method and a screening method for a substance having an action of activating / suppressing an innate immune mechanism, and a drug and a food for activating / suppressing the innate immune mechanism and a method of producing the same.BACKGROUND ART[0003]Higher vertebrate animals such as human beings have two types of immune mechanisms (immune system), i.e., an innate immunity and an acquired immunity, and are protected from infection by allowing both the immune mechanisms to act appropriately. Meanwhile, many remaining organisms such as insects do not have the acquired immune mechanism, and are protected from the infection by the innate immune mechanism alone.[0004]The innate immune mechanism is a defense mechanism against infection that the...

Claims

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Application Information

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Patent Type & Authority Patents(United States)
IPC IPC(8): A61K35/12A61K35/34A61K49/00C12Q1/02A23L33/10A23L33/105A61K36/18A61K36/23A61K36/28A61K36/31A61K36/42A61K36/81A61K36/896A61K36/8962A61K45/00A61K125/00A61P37/04G01N33/15G01N33/50
CPCA23L1/29A23L1/30A61K35/74A61K36/18A61K38/17C12N15/8509G01N33/5082A01K2267/0387C12N2710/14043A23L33/00A23L33/10A61P37/02A61P37/04
Inventor SEKIMIZU, KAZUHISAHAMAMOTO, HIROSHI
Owner IMAGINE GLOBAL CARE CORP
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