Rapid acting lactobacillus strains and their use to improve aerobic stability of silage
a technology of lactobacillus and lactobacillus, which is applied in the direction of microorganisms, biochemistry apparatus and processes, and bacteria based processes, can solve the problems of reducing the nutritional value of silage, affecting the aerobic stability of ensiled forage, and affecting the aerobic stability of silage, so as to improve the aerobic stability of ensiled forage, improve the aerobic stability, and facilitate the effect of aerobic exposur
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example 1
ing Lactobacillus Strains for Improving Silage Aerobic Stability in Corn
[0066]Studies were performed to develop a microbial inoculant comprising Lactobacillus species that can increase the fermentation and stabilization of whole plant corn silage to allow for early opening (aerobic exposure) at less than thirty (30) days post-ensiling.
Strain Selection:
[0067]Heterofermentative lactic acid bacterial cultures (252 isolates), taken from Pioneer Hi-Bred's microbial culture collection, were grown in De Man Rogosa Sharpe broth (MRS broth; Difco™Lactobacilli MRS; Becton Dickinson and Company, Sparks, Md. 21152 USA), prepared as described by the manufacturer, for 24 to 48 hours. An aliquot of cell suspension was transferred to an extract of whole plant corn forage broth (1:10 mixture of dried ground corn forage in water, autoclaved, 0.2-micron filter sterilized then 0.5% glucose added) and grown for 40 hours at 37 C.
[0068]After an initial screening process, five isolates were selected for fi...
example 2
ing Lactobacillus Strains for Improving Silage Aerobic Stability in Grasses
Strain Selection:
[0088]Heterofermentative lactic acid bacterial cultures (252 isolates), taken from Pioneer Hi-Bred's microbial culture collection, were grown in De Man Rogosa Sharpe broth (MRS broth; Difco™Lactobacilli MRS; Becton Dickinson and Company, Sparks, Md. 21152 USA), prepared as described by the manufacturer, for 24 to 48 hours. An aliquot of cell suspension was transferred to an extract of whole plant corn forage broth (1:10 mixture of dried ground corn forage in water, autoclaved, 0.2 micron filter sterilized then 0.5% glucose added) and grown for 40 hours at 37 C.
[0089]Strains were discovered and identified from corn or the feces of corn fed sheep samples taken in the United States. In 2012, three of the isolates were tested in European rye grass, and in 2013 only two isolates were tested as single strains and in combination with current commercial homofermentative strains LP286 and LP329.
Experi...
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