System Using Laser-Scatter Measurement Instrument For Organism Identification And Related Network

Abstract

An optical measurement instrument is an integrated instrument that includes an optical cavity with a light source, a sample cuvette, and an optical sensor. The instrument can be used for taking measurements of organism concentration in one or more samples. Preferably, the instrument holds multiple, individually-loaded, independent fluid samples and determines bacteria concentration via a forward-scattering signal. The instrument can incorporate onboard incubation to promote bacterial growth in the samples such that, once a certain bacterial concentration is achieved, the higher concentration sample can be used with a mass spectrometer to identify the type of bacteria. The instrument and mass spectrometer can be a part of a network for medical diagnostic testing data where data is stored in a manner that is inherently untainted by patient identifiable information.

Description

RELATED APPLICATIONS[0001]The present application claims priority to (i) U.S. Provisional Application Ser. No. 62 / 107,931, filed Jan. 26, 2015, titled “Multi-Sample Laser-Scatter Measurement Instrument With Incubation Feature” (ii) U.S. Provisional Application Ser. No. 62 / 100,800, filed Jan. 7, 2015, titled “System And Method For Detecting and Identifying Bacteria Type in a Fluid Sample,” (iii) U.S. Provisional Application Ser. No. 62 / 151,065, filed Apr. 22, 2015, titled “Networked Biological Data Collection System For Use With Laser-Scatter Measurement Instruments,” and (iv) U.S. application Ser. No. 14 / 562,304 titled “Cuvette Assembly Having Chambers for Containing Samples to be Evaluated through Optical Measurement,” filed on Dec. 5, 2014, each of which is herein incorporated by reference in entirety.COPYRIGHT[0002]A portion of the disclosure of this patent document may contain material which is subject to copyright protection. The copyright owner has no objection to the facsimil...

AI Technical Summary

Benefits of technology

[0020]In another aspect, the present invention is a method of identifying bacteria in a fluid sample, comprising (i) placing the fluid sample in a cuvette having a first window for receiving an input beam and a second window for transmitting a forward-scatter signal indicative of the presence or absence of the bacteria in the fluid sample, (ii) incubating the fluid sample in the cuvette within an optical-measuring instrument that provides the input beam, (iii) passing the input beam through the fluid sample while the cuvette is in the optical-measuring instrument, (iv) analyzing the forward-scatter signal from the fluid sample, (v) in response to the forward-scatter signal indicating the presence of bacteria in the fluid sample, continuing to incubat

Problems solved by technology

Optical laser scattering is one of the most sensitive methods, but its implementation can be very challenging, especially when analyzing biological samples in which suspended particles are relatively transparent in the medium.

In a common analytical method, culturing of the

Images