Cosmetic
a technology of cosmetics and ingredients, applied in the field of cosmetics, can solve the problems of chemical improvement of skin quality, lack of long-term effect of cosmetic formulations, and technical challenges, and achieve the effect of promoting skin rejuvenation
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example 1
[0026]A cosmetic cream is prepared after formulating the three phases. Phase A is heated to 75-80° C. with sufficient mixing to form a homogenous mixture. Phase B is premixed in a separate vessel at ambient temperature and added to Phase A at 40° C. or lower temperature. Phase C is added to the now combined A and B Phases mixture in order with sufficient mixing until a homogenous cream is formed. A homogeneous and storage stable cream results.
example 2
[0027]A cosmetic cream in which the fish spawn protein isolate and a natural product extract are components of Phase A is provided instead of the fish spawn protein isolate and the natural product extract present in a separate Phase C per Example 1. With Phase A being formed at ambient temperature to avoid protein denaturation, a like cream is obtained.
example 3
[0029]Fresh, unfertilized salmon (Salmo salar) eggs harvested from females in reproductive phase (late fall) are kept on ice, and the extract preferably made immediately. It is possible to freeze dry eggs in a cryoprotectant (e.g., 1.5 M 1,2-propanediol and 0.2 M sucrose) without disrupting the egg membrane. Freezing should be gradual (−1° C. / min) to −80° C. Eggs should be thawed and kept on ice throughout the extract preparation procedure.
[0030]Eggs are washed twice in HBSS or seawater with protease inhibitors (10 μg / ml). The washing solution is removed and the eggs are lysed and homogenized in a pre-chilled Dounce glass-glass homogenator. The lysate is transferred to Beckman Ultra Clear polymer centrifuge tubes (5 ml) while avoiding transfer of egg shells, and centrifuged for 15 min at 15,000 g at 4° C. in a Beckman ultracentrifuge using SW55T1 rotor. Three fractions are thereby obtained; lipid top fraction, cytoplasmic middle fraction, and a bottom fraction containing eggshells a...
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