Polymer Particle

Inactive Publication Date: 2013-08-15
DNA TRACER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent text describes a method for creating stable particles made of a polymer and nucleic acid molecules. The polymer and nucleic acid molecules are connected through strong bonds, making the particles more stable. The surface of the particles is coated with a hydrophilic and neutral group called poly(ethylene glycol) to minimize adsorption processes. The nucleic acid molecule can also be easily released on demand. These steps allow for the XLRP and XL2 to bind together prior to interacting with the polymer and nucleic acid. Overall, this method creates stable particles with nucleic acid molecules that are protected and secure.

Problems solved by technology

However, there are problems with this approach, primarily because of a limit to the number of readily distinguishable tags in existence.
The tracer particles in the prior art are not ideally suited for conducting such studies because the stability of the particle is not sufficient to protect and preserve a nucleic acid molecule in the environment for a sustained period of time.
Therefore, they would not yield accurate results for the movement of a material, wherein the sampling process takes place over a period of weeks or months following the addition of the tracer.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

Reaction of XLP-BMDB

[0096]The cationic cross-linking polymer, XLP, is firstly reduced to provide a free thiol (—SH) group. In a round bottom flask, equipped with a magnetic stirrer and nitrogen inlet, MBA / DMEDA22-SH (reduced XLP) polymer (72.5 mg, 50.26 mmol) was dissolved under an inert atmosphere in distilled water (14.5 ml). A two fold excess of 1,4-bismaleimidyl-2,3-dimhydroxybutane (BMDB) was used (compared to —SH of XLP, 6.10 mg). The BMDB was purchased from Pierce, Thermo Fisher Scientific, Loughborough, UK. The —SH groups were quantified using the Ellman's test (Ellman G. L 1959, Arch Biochem Biophys 82, 70-77). The reaction is shown in FIG. 3.

[0097]The BMDB was dissolved in DMSO (dimethylsulfoxide) or DMF (dimethylformamide) (4.676 ml). The BMDB solution was then added to the polymer solution, the pH was adjusted to 7 and the reaction mixture was allowed to react for 1 h at room temperature. The reaction mixture was ultrafiltered through a 1,000 nominal cut-off using distil...

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Abstract

A particle [1] comprising a nucleic acid molecule [2] and a poiyamicioamine (PAA) polymer [4], wherein both the nucleic acid molecule [2] and the PAA polymer [4] comprise a pendant disulphide, sulphydryl or activated sulphydryl moiety and are capable of cross-linking with each other. The particle further comprises stabilising cross-linkers [6]& [7].

Description

FIELD OF THE INVENTION[0001]The invention relates to a polymer particle comprising a nucleic acid molecule and a polyamidoamine polymer. It also relates to a method of making the particle, and to a method of marking a material with the particle and determining whether a material has been marked. The use of the particle in marking a material, e.g. a liquid such as groundwater, is also an aspect of the invention.BACKGROUND[0002]There are many circumstances in which it is useful to track the movement of a material in the environment, for example, in groundwater studies. Groundwater studies can be used to determine the direction and / or velocity of groundwater movement, as well as potential pollutants that could contaminate, and be carried by, the water.[0003]It is known that groundwater tracers can be naturally occurring, e.g. heat carried by a stream of geothermal water, or minerals that have been leached from the surrounding environment. Alternatively, tracers can be introduced, e.g. ...

Claims

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Application Information

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IPC IPC(8): C08G69/48
CPCC08G73/028C08L79/02C08J3/12Y10T428/2982C08J2379/02C08G69/48C08J3/24C08J3/246
Inventor GARNETT, MARTIN
Owner DNA TRACER TECH
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