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Vaccines and Methods for Using the Same

a technology of vaccines and methods, applied in the field of improved vaccines, can solve the problems of killing or inactivating vaccines, sub-unit vaccines containing only proteins, and inducing poor cellular immune responses

Inactive Publication Date: 2008-11-06
THE TRUSTEES OF THE UNIV OF PENNSYLVANIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0026]The present invention further relates to recombinant vaccines comprising a nucleotide sequence that encodes an immunogen operably linked to regulatory elements, a nucleotide sequences that encode one or more chemokines selected from the group consisting of: CTACK, TECK, MEC and functional fragments thereof, and to methods of inducing an immune response, including methods of inducing a mucosal immune response, in an individual against an immunogen comprising administering such a recombinant vaccine to an individual.
[0027]The present invention further relates to a live attenuated pathogen comprising a nucleotide sequence that encodes one or more chemokines selected from the group consisting of: CTACK, TECK, MEC and functional fragments thereof, and to methods of inducing an immune response, including methods of inducing a mucosal immune response, in an individual against a pathogen comprising administering the live attenuated pathogen to an individual.
[0028]The present invention further relates to methods of inducing an immune response in an individual against an immunogen comprising administering to said individual one or more of CTACK protein, TECK protein, MEC protein and functional fragments thereof in combination with an isolated nucleic acid molecule that encodes an immunogen; and/or a recombinant vaccine that encodes an immunogen and/or a subunit vaccine that comprises an immunogen and/or a live attenuated vac

Problems solved by technology

On the other hand, killed or inactivated vaccines, and sub-unit vaccines which comprise only proteins do not induce good cellular immune responses although they do induce a humoral response.

Method used

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  • Vaccines and Methods for Using the Same
  • Vaccines and Methods for Using the Same
  • Vaccines and Methods for Using the Same

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example

[0121]Plasmids were constructed using the pVax1 (Invitrogen) backbone, inserting coding sequences for murine CTACK, murine TECK or murine MEC into the multiple cloning region of pVax1, placing the coding sequences under the regulatory control of the cytomegalovirus promoter and bovine growth hormone polyadenylation signal. (FIG. 2) Inserts were confirmed by restriction digest conformation (FIG. 2). RD cells were transfected with vector or construct (pCTACK or pTECK) to confirm that the coding sequences would be expressed. Testing was not done to confirm pMEC expression as antibodies against murine MEC were not available. The data shown in FIG. 2 confirms that CTACK and TECK was expressed.

[0122]Balb / C mice (N=4 per group) were immunized with vector only, plasmid pHIV-1 gag only (pVax-1 with HIV-1 gag inserted at multiple cloning site), plasmid pHIV-1 gag+pCTACK, plasmid pHIV-1 gag+pTECK and plasmid pHIV-1 gag+pMEC on days 0 and 14. Mice were sacrificed at day 21 and spleens were remo...

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Abstract

Compositions comprising one or more isolated nucleic acid molecules that encode an immunogen in combination with one or more of CTACK protein, TECK protein, MEC protein and functional fragments thereof and / or an isolated nucleic acid molecule that encodes an protein selected from the group consisting of: CTACK, TECK, MEC and functional fragments thereof are disclosed. Methods of inducing an immune response, including methods of inducing mucosal immune responses, in an individual against an immunogen, using such compositions are disclosed.

Description

FIELD OF THE INVENTION[0001]The present invention relates to improved vaccines, improved methods for inducing immune responses, including mucosal immune responses, and for prophylactically and / or therapeutically immunizing individuals against immunogens.BACKGROUND OF THE INVENTION[0002]Infectious agents commonly enter the host across a mucosal tissue such as the oral mucosa and other mucosa of the alimentary canal, the respiratory tract including olfactory and conjunctival mucosa, the mammary glands, and the genitourinary tract. The mucosal immune system provides a secretory immunoglobulin response to prevent infectious agents at these points of entry.[0003]The secretory immune response includes clonal proliferation of antigen-specific B cells and progressive isotype switching by the B cell progeny to all subclasses of IgG- and IgA-secreting cells. Antigens such as microorganisms, proteins, polysaccharides, etc., that are encountered at a mucosal site can elicit local production of ...

Claims

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Application Information

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IPC IPC(8): A61K39/00C07H21/04A61K39/285A61K39/21
CPCA61K39/21A61K39/39A61K2039/53A61K2039/541A61K2039/55516C07K14/005C12N2740/16222C12N2740/16234A61K39/12A61P37/02Y02A50/30
Inventor WEINER, DAVID BKUTZLER, MICHELE
Owner THE TRUSTEES OF THE UNIV OF PENNSYLVANIA
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