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T Cell Apoptosis Inducer

Inactive Publication Date: 2008-07-10
CALPIS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0009]The inducer of T cell apoptosis according to the present invention is safe even if it is orally taken for a long period of time, because of long experience of usage of lactic acid bacteria as starter cultures for fermentation foods such as yoghurt, pickles and the kind of stuff. In addition, it is expected that the inducer of the invention induces apoptosis of excess T cells to prevent and / or cure an inflammatory disease, such as an organ-specific autoimmune disease or an allergy caused by excess T cells.

Problems solved by technology

The imbalance in the Th1 / Th2 ratio may cause various immune diseases.

Method used

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Examples

Experimental program
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Effect test

example 1

Preparation of Lactic Acid Bacteria

[0034]Lactic acid bacteria belonging to the genus Lactobacillus and lactic acid bacteria belonging to the genus Bifidobacterium were cultured at 37° C. for 18 hours in MRS medium and GAM medium, respectively. After the culture, the lactic acid bacterial cells were collected by centrifugation, washed and lyophilized. The dried bacterial cells were suspended in a PBS solution, treated by heating at 100° C. for 10 minutes and used in the following experiments.

example 2

Induction of Apoptosis of Naive T Cells

[0035]Preparation of cells: The spleen was excised from a DO11.10 TCR-transgenic mouse bearing αβ-T cell receptor (TCR) gene derived from a T cell clone DO11.10 that I-Ad restrictively recognizes a region between the 323rd and 339th residues of ovalbumin (hereinafter referred to as OVA), and a single cell suspension was prepared. CD4 microbeads (Militenyi Biotec) suspended in MACS buffer (PBS containing 0.5% bovine serum albumin and 2 mM EDTA) were reacted with the cell suspension at 4° C. for 15 minutes. After the cells were washed, positive selection was collected using a magnetic separation column (Militenyi Biotec) to prepare CD4-positive T cells. The spleen was excised from a BALB / c mouse and a single cell suspension was prepared. Thy1.2 microbeads (Militenyi Biotec) suspended in MACS buffer were reacted with the cell suspension at 4° C. for 15 minutes. After the cells were washed, the negatively selected cell fraction was collected using ...

example 3

T Cell Apoptosis in Dose-Dependent Manner

[0039]The preparation of cells and the detection of apoptosis were carried out in the same manner as in Example 2. The cells were cultured in accordance with Example 2, except that Lactobacillus acidophilus L-92 was used at concentrations of 0.1, 1.0 and 10 μg / ml as heat-killed lactic acid bacterial preparations.

[0040]The test results are shown in FIG. 2. It was observed that Lactobacillus acidophilus L-92 induces T cell apoptosis in a dose-dependent manner.

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Abstract

An inducer of T cell apoptosis and a health food for inducing T cell apoptosis comprising as an active ingredient of a lactic acid bacterium belonging to the genus Lactobacillus or the genus Bifidobacterium are disclosed. Particularly preferred lactic acid bacteria belonging to the genus Lactobacillus or the genus Bifidobacterium are Lactobacillus acidophilus L-92 (FERM BP-4981), Lactobacillus amylovorus CP1750 (FERM BP-10532), Bifidobacterium catenulatum CP2829 (FERM BP-10533) and Bifidobacterium longum CP760 (FERM BP-10531). The inducer of T cell apoptosis of the invention is useful in the prevention and cure of a disease such as an organ-specific autoimmune disease or type I allergy.

Description

TECHNICAL FIELD[0001]The present invention relates to an inducer of apoptosis of T cells, which comprises a lactic acid bacterial cell as an active component.BACKGROUND ART[0002]The mammalian immune system protects the host by recognizing bacteria, viruses and kinds of stuff as antigens and eliminating them through the action of the system. The immune system is roughly classified into cellular immunity and humoral immunity, and the both immune systems affect each other to optimize the immune reactions for host defense. T cells participate to play an important role in the modulation of the function of the immune system.[0003]T cells are broadly classified into Th1 and Th2 cells. Th1 cells enhance cellular immunity and Th2 cells enforce humoral immunity. The imbalance in the Th1 / Th2 ratio may cause various immune diseases. It is considered that when Th1 cells are dominant, an organ-specific autoimmune disease occurs and when Th2 cells are dominant, a type I allergy or related diseases...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/10A23L33/135A61K35/74A61K35/745A61K35/747A61P1/04A61P3/10A61P5/14A61P17/00A61P19/02A61P25/00A61P29/00A61P37/02A61P37/08A61P43/00
CPCA23L1/3014A23Y2220/05A23Y2300/55C12R1/23A61K35/747C12N1/20C12R1/225A61K35/745A23L33/135A61P1/04A61P17/00A61P17/02A61P19/02A61P25/00A61P29/00A61P3/10A61P37/02A61P37/08A61P43/00A61P5/14C12R2001/23C12N1/205C12R2001/225A23V2400/115A23V2400/533
Inventor FUJIWARA, SHIGERUKANZATO, HIROKIHACHIMURA, SATOSHI
Owner CALPIS
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