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Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation

a polycyclic sugar surrogate and oligomeric compound technology, applied in the field of modified oligomers, can solve the problems of dsrna activity not being as effective as morpholino oligomer, rnai activity being deleterious,

Inactive Publication Date: 2008-02-14
ALLERSON CHARLES +6
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, substitution with 2′-deoxynucleosides or 2′-OMe-nucleosides throughout the sequence (sense or antisense) was shown to be deleterious to RNAi activity.
The morpholino oligomer did show activity but was not as effective as the dsRNA.

Method used

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  • Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation
  • Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation
  • Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation

Examples

Experimental program
Comparison scheme
Effect test

example 1

Synthesis of Nucleoside Phosphoramidites

[0398] The following compounds, including amidites and their intermediates were prepared as described in U.S. Pat. No. 6,426,220 and published PCT WO 02 / 36743; 5′-O-Dimethoxytrityl-thymidine intermediate for 5-methyl dC amidite, 5′-O-Dimethoxytrityl-2′-deoxy-5-methylcytidine intermediate for 5-methyl-dC amidite, 5′-O-Dimethoxytrityl-2′-deoxy-N4-benzoyl-5-methylcytidine penultimate intermediate for 5-methyl dC amidite, [5′-O-(4,4′-Dimethoxytriphenylmethyl)-2′-deoxy-N4-benzoyl-5-methylcytidin-3′-O-yl]-2-cyanoethyl-N,N-diisopropylphosphoramidite (5-methyl dC amidite), 2′-Fluorodeoxyadenosine, 2′-Fluorodeoxyguanosine, 2′-Fluorouridine, 2′-Fluorodeoxycytidine, 2′-O-(2-Methoxyethyl) modified amidites, 2′-O-(2-methoxyethyl)-5-methyluridine intermediate, 5′-O-DMT-2′-O-(2-methoxyethyl)-5-methyluridine penultimate intermediate, [5′-O-(4,4′-Dimethoxytriphenylmethyl)-2′-O-(2-methoxyethyl)-5-methyluridin-3′-O-yl]-2-cyanoethyl-N,N-diisopropylphosphoramidit...

example 2

Oligonucleotide and Oligonucleoside Synthesis

[0399] Oligonucleotides: Unsubstituted and substituted phosphodiester (P═O) oligonucleotides are synthesized on an automated DNA synthesizer (Applied Biosystems model 394) using standard phosphoramidite chemistry with oxidation by iodine.

[0400] Phosphorothioates (P═S) are synthesized similar to phosphodiester oligonucleotides with the following exceptions: thiation was effected by utilizing a 10% w / v solution of 3,H-1,2-benzodithiole-3-one 1,1-dioxide in acetonitrile for the oxidation of the phosphite linkages. The thiation reaction step time was increased to 180 sec and preceded by the normal capping step. After cleavage from the CPG column and deblocking in concentrated ammonium hydroxide at 55° C. (12-16 hr), the oligonucleotides were recovered by precipitating with >3 volumes of ethanol from a 1 M NH4OAc solution. Phosphinate oligonucleotides are prepared as described in U.S. Pat. No. 5,508,270, herein incorporated by reference.

[04...

example 3

RNA Synthesis

[0411] In general, RNA synthesis chemistry is based on the selective incorporation of various protecting groups at strategic intermediary reactions. Although one of ordinary skill in the art will understand the use of protecting groups in organic synthesis, a useful class of protecting groups includes silyl ethers. In particular bulky silyl ethers are used to protect the 5′-hydroxyl in combination with an acid-labile orthoester protecting group on the 2′-hydroxyl. This set of protecting groups is then used with standard solid-phase synthesis technology. It is important to lastly remove the acid labile orthoester protecting group after all other synthetic steps. Moreover, the early use of the silyl protecting groups during synthesis ensures facile removal when desired, without undesired deprotection of 2′ hydroxyl.

[0412] Following this procedure for the sequential protection of the 5′-hydroxyl in combination with protection of the 2′-hydroxyl by protecting groups that ...

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Abstract

Compositions comprising first and second oligomers are provided wherein at least a portion of the first oligomer is capable of hybridizing with at least a portion of the second oligomer, at least a portion of the first oligomer is complementary to and capable of hybridizing to a selected target nucleic acid, and at least one of the first or second oligomers includes a modification comprising a polycyclic sugar surrogate. Oligomer / protein compositions are also provided comprising an oligomer complementary to and capable of hybridizing to a selected target nucleic acid and at least one protein comprising at least a portion of an RNA-induced silencing complex (RISC), wherein at least one nucleoside of the oligomer has a polycyclic sugar surrogate modification.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] The present application is a continuation of U.S. Ser. No. 10 / 701,285 filed Nov. 4, 2003, which claims benefit to U.S. Provisional Application Ser. No. 60 / 423,760 filed Nov. 5, 2002, which are incorporated herein by reference in its entirety.FIELD OF THE INVENTION [0002] The present invention provides modified oligomers that modulate gene expression via a RNA interference pathway. The oligomers of the invention include one or more modifications thereon resulting in differences in various physical properties and attributes compared to wild type nucleic acids. The modified oligomers are used alone or in compositions to modulate the targeted nucleic acids. In preferred embodiments of the invention, the modifications include replacement of the sugar moiety with a polycyclic sugar surrogate. BACKGROUND OF THE INVENTION [0003] In many species, introduction of double-stranded RNA (dsRNA) induces potent and specific gene silencing. This phenome...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07H21/04C07H21/00C12N15/113C12Q1/68
CPCC07H21/00C07H21/04C12N15/111C12N15/113C12N2320/51C12N2310/321C12N2310/322C12N2310/323C12N2310/14C12N2310/343C12N2310/3521C12N2310/3533
Inventor ALLERSON, CHARLESBHAT, BALKRISHENELDRUP, ANNE B.MANOHARAN, MUTHIAHGRIFFEY, RICHARDBAKER, BRENDA F.SWAYZE, ERIC E.
Owner ALLERSON CHARLES
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