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Compositions and methods for promoting wound healing and tissue repair

a tissue repair and composition technology, applied in the field of tissue repair, can solve the problems of reducing the chances of mortality and morbidity, impaired wound healing, and significant health care problems, and achieve the effects of stimulating wound repair, accelerating wound healing, and accelerating wound healing

Inactive Publication Date: 2007-05-17
UNITED STATES OF AMERICA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is based on the discovery that a protein called thymosin β4 (Tβ4) can accelerate wound healing and promote tissue repair. This discovery has led to the development of methods for treating wound healing disorders by administering Tβ4 or its variants to patients with wounds. The invention also provides pharmaceutical compositions containing Tβ4 for this purpose. The technical effects of the invention include promoting faster wound healing, stimulating epithelial cell migration, and diagnosing and treating wound healing disorders associated with Tβ4.

Problems solved by technology

Inadequate methods and compositions to effectively heal chronic wounds is a significant health care problem.
Impaired wound healing increases the chances of mortality and morbidity.
This problem is especially prominent in patients with diabetes who develop severe, life threatening wounds on body extremities.
Chronic diabetic foot ulcers often lead to amputations.
These wounds are often the result of poor circulation derived from the diabetic patients' insulin-compromised cells as well as impaired vascularization of the wound bed, reduced infiltration of germ fighting cells and reduced tissue epithelialization.
Although the basic process of tissue repair has been characterized, the individual steps and factors necessary to carry out this complex series of events are not well understood.
Though informative, such a test does not mimic the dynamic in vivo wound healing conditions to the extent that not all factors involved in wound closure are present in the in vitro assay.
Furthermore, such an increase in PDGF-AA is not seen in chronic non-healing wounds.
However, most of these growth and angiogenic factors have side effects.

Method used

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  • Compositions and methods for promoting wound healing and tissue repair
  • Compositions and methods for promoting wound healing and tissue repair
  • Compositions and methods for promoting wound healing and tissue repair

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0096] In vivo wound healing is accelerated by Tβ4

[0097] Tβ4, whether administered topically or intraperitoneal, significantly accelerated wound healing as compared to untreated wounds (FIG. 2 and 3). Full thickness 8 mm punch biopsy wounds were made on the dorsal surface of rats as previously reported (Bhartiya et al., J. Cell. Physiol. 150:312, 1992; Sihhu et al., J. Cell. Physiol. 169:108, 1996) and Tβ4 was given topically at the time of wounding (5 μg in 50 μl) and again after 48 hours. Controls for the topical treatment received identical amounts of saline at the time of wounding and at 48 hours. Additional rats received intraperitoneal injections at the time of wounding (60 μg in 300 μl) and again every other day (e.g., days 0, 2, 4, and 6). Controls for these animals received identical amounts of saline intra-peritoneally on the same injection schedule. On days 4 and 7 post-wounding, measurements were made on the wound size. At days 8 and 9 post-wounding, tissue was collecte...

example 2

[0104] Migration Assays of Keratinocytes

[0105] Primary keratinocytes were prepared from either Balb / c or CD-1 newborn mice as described previously (Dlugosz et al., 1995). Cells were plated in calcium- and magnesium-free Eagle's Minimal Essential Medium (EMEM) containing 8% fetal calf serum treated with 8% Chelex (Bio-Rad Laboratories, Hercules, Calif.), 20 units / ml penicillin-streptomycin, and the calcium concentration was adjusted to 0.25 mM. The following day, cultures were washed with calcium- and magnesium-free phosphate buffered saline, treated briefly with Trypsin (Life Technologies, Gaithersburg, Md.), washed with culture medium and resuspended in EMEM containing 0.05 mM calcium. Cells were used immediately in migration assays.

[0106] Keratinocyte migration assays were carried out in Boyden chamber using 12 μm pore polyester membranes (Poretics, Livermore, Calif.) coated with a 0.1 mg / ml solution of collagen IV in dH20 (Trevigen, Gaithersburg, Md.). Filters were then dried a...

example 3

[0108] Migration Assays of Corneal Epithelial Cells

[0109] Corneal Epithelial Cell migration assays were carried out in Boyden chamber using 12 μm pore polyester membranes (Poretics, Livermore, Calif.) coated with a 0.1 mg / ml solution of collagen IV in dH20 (Trevigen, Gaithersburg, Md.). Filters were then dried at least 1 h. Cells were cultured and resuspended in Eagle's Minimal Essential Medium with 0.05 mM Ca2+. The bottom chamber was loaded with EMEM containing 0.01, 0.1, 10, 100, and 1000 ng / ml of synthetic Tβ4. Conditioned medium from primary dermal fibroblasts and / or keratinocyte growth factor was added to several wells as a positive control. Cells were added to the upper chamber at a concentration of 50,000 cells per well. Chambers were incubated at 35 C / 7% CO2 for 4-5 hours and the filters were then fixed and stained using Diff-Quik (Baxter Healthcare Corporation, McGraw Park, Ill.). The cells that migrated through the filter were quantitated by counting the center of each w...

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Abstract

The present invention relates to methods for promoting tissue repair, angiogenesis and cell migration. The method of the invention utilizes thymosin β4 (Tβ4) peptide to promote tissue repair, angiogenesis and cell migration. The invention further relates to modulating Tβ4 activity in tissues.

Description

CROSS REFERENCE TO RELATED APPLICATIONS [0001] This application claims priority from Provisional Application Serial No. 60 / 094,690, filed Jul. 30, 1998, which is incorporated herein by reference in its entirety and to which application a priority claim is made under 35 U.S.C. §119(e).STATEMENT AS TO FEDERALLY SPONSORED RESEARCH [0002] This invention was made in part with funds from the National Institutes of Health, Intramural Program. The government may have certain rights in this invention.TECHNICAL FIELD OF THE INVENTION [0003] The present invention relates generally to tissue repair and more specifically to methods of wound healing using thymosin β4. BACKGROUND OF THE INVENTION [0004] Inadequate methods and compositions to effectively heal chronic wounds is a significant health care problem. Impaired wound healing increases the chances of mortality and morbidity. This problem is especially prominent in patients with diabetes who develop severe, life threatening wounds on body ex...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/18A61K38/08A61K38/22A61K39/395A61P17/02A61P29/00C07K16/26
CPCA61K38/1841A61K38/08A61K38/2292A61K2300/00Y10S930/18A61P17/02A61P29/00A61P9/00A61P9/10A61K9/0048A61K45/06
Inventor KLEINMAN, HYNDA K.GOLDSTEIN, ALLAN L.MALINDA, KATHERINE M.SOSNE, GABRIEL
Owner UNITED STATES OF AMERICA
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