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Multifunctional molecular complexes for gene transfer to cells

Inactive Publication Date: 2005-10-27
WYETH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0035] The present invention also relates to methods of treating an individual suffering from an autoimmune disease comprising the steps of contacting cells of said individual with a multifunctional molecular complex which includes a nucleic acid composition, as detailed further above, thereby administering to cells of said individual,

Problems solved by technology

However, DNA by itself is hydrophilic, and the hydrophobic character of the cellular membrane poses a significant barrier to the transfer of DNA across it.

Method used

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  • Multifunctional molecular complexes for gene transfer to cells
  • Multifunctional molecular complexes for gene transfer to cells
  • Multifunctional molecular complexes for gene transfer to cells

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of N4-5′-Aminopentylspermidine Hydrochloride 8

N4-(4-cyanobutyl)-N1,N8-Bis(tert-butyloxycarbonyl)-S-permidine (6)

[0252] A solution of N1,N8-Bis(tert-butyloxycarbonyl) spermidine (2.92 g, 8.45 mmol, 1.0 eq) [S.Nagarajan and B. Ganem, J. Org. Chem., 50, 5735-37 (1985)] in acetonitrile (125 mL) was treated with N,N-diisopropylethylamine (3.534 mL, 20.0 mmol, 2.4 eq), potassium iodide (2.81 g, 16.90 mmol, 2.0 eq), and 5-chlorovaleronitrile (1.902 mL, 16.90 mmol, 2.0 eq). The resulting homogeneous solution was heated to reflux for 2 hours. The mixture was treated with additional N,N-diisopropylethylamine (1.767 mL; 1.2 eq), potassium iodide (1.41 g, 1.0 eq) and 5-chlorovaleronitrile (0.951 mL, 1.0 eq), and refluxed an additional 18 hours. Thin layer chromatography (TLC) indicated no remaining starting material. The acetonitrile was removed under vacuum, and the residue taken up in chloroform (250 mL). This solution was washed with water (200 mL), dried (Na2SO4), and stripped...

example 2

Preparation of N4-(5-(P-3′-propionyl galactosyl-β1″-4′-thioglucoside) aminopentyl)speridine 9

S-(Succinimidyl-β-3′-propionyl)hepta-O-acetyl galactosyl-β1′4-thioglucoside (10)

[0255] A solution of S-β-3-propionyl hepta-O-acetyl galactosyl-β1-4-thioglucoside [M. Elofsson, S. Roy, B. Walse and J. Kihlberg, Carb. Res., 246, 89-103 (1993)] (4.60 g, 6.35 mmol) in 1:1 isopropanol / chloroform (100 mL) was treated with N-hydroxysuccinimide (0.73 g, 6.35 mmol) and N,N′-dicyclohexylcarbodiimide (1.31 g, 6.35 mmol). After stirring at room temperature for 19 hours, the mixture was cooled to 40 for 1 h and filtered. The solvent was removed from the filtrate under vacuum to give a white solid that was recrystallized from 2-propanol (3.43 g). The isolated product was 92% pure by high performance liquid chromatography (HPLC). 1H NMR (CDCL3): δ 2.0-2.16 (7s, 21.0H), 2.85 (s, 3.8H), 2.85-3.1 (m, 4.2H), 3.65 (m, 0.7H), 3.78 (t, 1.0H), 3.88 (t, 1.0H), 4.11 (m, 4.0H), 4.54 (m, 2.8H), 4.97 (m, 1.8H), 5.11 ...

example 3

Preparation of N4-(5-[N2,N6-bis(P-3′-propionyl galactosyl-p 1-4-thioglucoside)lysyl-N6(β-3′-propionyl galalactosyl-β1-4-thioglucoside)lysyl]-amino)pentylspermidine Acetate Salt 18

[0259] For comparative purposes, the CAS style name of the compound 18 set out above is as follows: 4-[(N[2[N2,N6-bis(3-[4-O(β-D-galactopyranosyl)-β-D-glucopyranosylthio]propionyl)lysyl]N6-(3-[4-O-(β-D-galactopyranosyl)-β-D-glucopyranosylthio]-propionyl)lysinamido)pentyl]-1,8-diamino-4-azaoctane, Acetate Salt.

N4-(5-[N2,N6-bis(β-3′-propionyl hepta-O-acetyl galactosyl-β1-4-thioglucoside)lysyl N6-(β-3′-propionyl hepta-O-acetyl galactosyl-β1-4-thioglucoside) lysine (14)

[0260] To a solution of lysyl-lysine (0.25 g, 0.64 mmol) in 1:1 water / acetonitrile (100 mLs) was added N,N-diisopropylethylamine (0.336 mls, 1.95 mmol, 3.0 eq) and compound 10 (1.859, 2.25 mmol) and stirred at room temperature for a few minutes until homogeneity was achieved. The pH was closely monitored at regular intervals, and N,N-diisoprop...

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Abstract

A multifunctional molecular complex for the transfer of a nucleic acid composition to a target cell is provided. The complex is comprised of A) said nucleic acid composition and B) a transfer moiety comprising 1) one or more cationic polyamines bound to said nucleic acid compositions, 2) one or more endosome membrane disrupting components attached to at least one nitrogen of the polyamine and 3) one or more receptor specific binding components.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a continuation of U.S. patent application Ser. No. 10 / 010,114, filed Nov. 13, 2001, which is a divisional of U.S. patent application Ser. No. 09 / 425,597, filed Oct. 22, 1999, now U.S. Pat. No. 6,379,965, issued Apr. 30, 2002, which is a divisional of U.S. patent application Ser. No. 08 / 809,397, filed Mar. 21, 1997, now U.S. Pat. No. 6,127,170, issued Oct. 3, 2000, which is a 35 USC §371 of PCT / US95 / 12502, filed Sep. 28, 1995, which claims the benefit of the priority of U.S. patent application Ser. No. 08 / 314,060, filed Sep. 28, 1994, now U.S. Pat. No. 5,837,533, issued Nov. 17, 1998.BACKGROUND OF THE INVENTION [0002] The present invention is in the field of methods for the transfer of genetic information, e.g., foreign DNA, into target cells, especially eukaryotic cells. In particular, the present invention relates to nonviral gene carriers comprising multifunctional molecular conjugates which include, inter alia, li...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K48/00C12N15/09C07C211/14C07C233/35C07C271/22C07K7/08C07K14/005C07K14/11C12N15/64C12N15/86C12N15/87C12N15/88
CPCA61K47/48046A61K47/48123A61K48/00C12N2760/16022C12N15/64C12N15/87C07K14/005A61K47/543A61K47/554
Inventor BOUTIN, RAYMOND
Owner WYETH
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