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Sequence-specific inhibition of small RNA function

a function and sequence-specific technology, applied in the field of sequence-specific inhibition of small rna function, can solve the problems of unfavorable understanding of the mechanisms by which these processes occur

Active Publication Date: 2005-10-13
MASSACHUSETTS UNIV OF
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0006] The present invention is based, at least in part, on the discovery that RISC inactivators are potent and irreversible inhibitors of small RNA-directed RNA silencing in vivo and in vitro. In particular, the invention is based, at least in part, on the discovery that 2′-O-methyl oligonucleotides are potent and irreversible inhibitors of small RNA-directed RNA silencing in vivo and in vitro. Accordingly, the present invention relates to methods of modulating (e.g., inhibiting) RNA silencing, in particular, microRNA (miRNA)-mediated and/or siRNA-mediated RNA silencing. The RNA silencing-inhibitory agents of the invention are suitable for use in modulating

Problems solved by technology

Despite the widespread use of RNAi to ‘knock down’ gene function and the increasing body of evidence supporting a role for miRNAs in RNA silencing, the mechanisms by which these processes occur are not yet fully understood.

Method used

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Examples

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Effect test

example 1

Inhibition of RNAi by 2′-O-methyl Oligonucleotides

[0325] Although RNAi has proved a straightforward and cost-effective method to assess the function of protein-coding mRNAs (Fire et al., 1998; Caplen et al., 2000; Caplen et al., 2001; Carthew, 2001; Elbashir et al., 2001b) and even some non-coding RNAs (Liang et al., 2003), no comparable method allows the sequence-specific inactivation of the siRNA or miRNA components of the RISC. The invention features such inhibitors. Preferred inhibitors of RISC function are nucleic acid-based molecules that are recognized by the RISC by nucleotide complementarity, but are refractory to RISC-directed endonucleolytic cleavage or translational control. Such molecules are designed such that they are capable of titrating out RISC complexes containing a complementary siRNA or miRNA, but have little or no effect on the function of RISC complexes containing guide RNAs unrelated in sequence. Such RISC inhibitors can further be designed such that they ar...

example 2

Inhibition of RNAi in Cultured Human Cells

[0331] The data presented in Example 1 showed that 2′-O-methyl oligonucleotides were stoichiometric, irreversible, sequence-specific inhibitors of siRNA function in RNAi reactions using Drosophila embryo lysate. To address the question of whether 2′-O-methyl oligonucleotides could block siRNA function in vivo, sequential transfection experiments were performed using 1, 5, 10 or 25 nM siRNA duplex. siRNA was transfected on the first day, then reporter and control plasmids cotransfected together with various amounts of 2′-O-methyl oligonucleotide on the second day. Silencing of Pp-luc, relative to the Rr-luc control was measured on the third day. For each siRNA concentration, the concentration of 2′-O-methyl oligonucleotide required for half-maximal inhibition of RNAi was determined (FIG. 4A-D). Increasing amounts of the 2′-O-methyl oligonucleotide gradually extinguished the ability of the siRNA to silence Pp-Luc in all four experiments. The ...

example 3

Inhibition of miRNA Function In Vitro and In Vivo

[0332] In animal cells, miRNAs are thought predominantly to function as translational regulators. Nonetheless, a growing body of evidence suggests that they function through a similar, if not identical, RISC as siRNAs (Hutvágner and Zamore, 2002; Zeng et al., 2002; Doench et al., 2003; Khvorova et al., 2003; Schwarz et al., 2003; Zeng et al., 2003b). Because 2′-O-methyl oligonucleotides blocked siRNA function in vitro and in cultured human cells, it was asked if these oligonucleotides might likewise disrupt the function of a specific miRNA in vitro and;in vivo. An ideal candidate for such an miRNA is let- 7. Classical genetic mutations in C. elegans let-7 produce well characterized, readily scored phenotypes. Furthermore, human HeLa cells express multiple let-7 family members (Rfam Accession numbers MI0000060-MI0000068, MI0000433 and MI0000434), and endogenous let-7 is present naturally in RISC (Hutvágner and Zamore, 2002; Zeng and C...

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Abstract

The present invention relates to the discovery of a method for inhibiting RNA silencing in a target sequence-specific manner. RNA silencing requires a set of conserved cellular factors to suppress expression of gene-encoded polypeptide. The invention provides compositions for sequence-specific inactivation of the RISC component of the RNA silencing pathway, and methods of use thereof. The RISC inactivators of the present invention enable a variety of methods for identifying and characterizing miRNAs and siRNAs, RISC-associated factors, and agents capable of modulating RNA silencing. Therapeutic methods and compositions incorporating RISC inactivators and therapeutic agents identified through use of RISC inactivators are also featured.

Description

RELATED APPLICATIONS [0001] This application claims the benefit of U.S. Ser. No. 60 / 543,796, entitled “Sequence-Specific Inhibition of Small RNA Function,” filed on Feb. 10, 2004, and U.S. Ser. No. 60 / 525,474, entitled “Sequence-Specific Inhibition of Small RNA Function,” filed on Nov. 26, 2003. The entire contents of these applications are hereby incorporated herein by reference.STATEMENT AS TO FEDERALLY SPONSORED RESEARCH [0002] Funding for the work described herein was at least in part provided by the federal government (N.I.H. grants GM62862-01 and GM65236-01, and GM58800).BACKGROUND OF THE INVENTION [0003] The endoribonuclease Dicer produces two types of small regulatory RNAs that regulate gene expression: small interfering RNAs (siRNAs) and microRNAs (miRNAs) (Bernstein et al., 2001; Grishok et al., 2001; Hutvágner et al., 2001; Ketting et al., 2001; Knight and Bass, 2001). In animals, siRNAs direct target mRNA cleavage (Elbashir et al., 2001c; Elbashir et al., 2001d), whereas...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/85C12QC12Q1/68
CPCC12N15/111C12N2310/11C12N2310/321C12N15/113C12N2320/50C12N2310/3521A61P21/00A61P35/00A61P3/10A61K31/7115C12N15/11A61K31/7105A61K31/712C12N2310/141C12N2310/113C12Q1/6897
Inventor HUTVAGNER, GYORGYZAMORE, PHILLIP
Owner MASSACHUSETTS UNIV OF
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