Tissue staining protocol and composition

Abstract

An enhanced and particularly quick histological staining method is expressed in which a frozen tissue or deparafffinized tissue sample is briefly immersed in an eosin solution, used as a first dye, and then in a hematoxylin solution, used as a second die. The slide is rinsed and air-dried to make ready for assessment.

Description

FIELD OF INVENTION [0001] The present invention generally relates to a novel combination for staining histological slides of cells, tissues, and organs, and more particularly, to a method of staining comprising of a combination where eosin is applied as first dye and hematoxylin is applied as a second dye. More particularly for a combination for staining that will reveal new chemical components and structures in cells, tissue, and organ samples not seen with a conventional hematoxylin and eosin stain. BACKGROUND OF THE INVENTION [0002] In order for the scientific community to gain rapid increase in the information of diseases, precise diagnosis is needed to maximize efficiency of treatments. Histology and cytology have been two popular diagnostic techniques that scientists utilize to gain insight into the structures and mechanism of cells and tissues. Both techniques involve the staining of tissue cells to distinguish different parts of the cells. However, these techniques, as prese...

AI Technical Summary

Benefits of technology

[0013] In one phase, the present invention supplies a new solution that is effective in staining histological slides of cells, tissues, and organs. The solution comprises of a combination, of eosin and hematoxylin, where eosin is applied to a specimen first, and hematoxylin is applied as a second dye upon the specimen. This combination employed by the present invention, will bring forth new information through histological slides in teaching or studying, research, and diagnostic fields.

[0014] In a second phase, the present invention relates to two histological staining methods. In the methods provided by the present invention, the combination of eosin and hematoxylin is applied to a specimen to provide a stained cell or tissue sample. The present invention also supplies a method containing the procedure of staining the specimen with the combination solution noted previously to provide a stained specimen. The stained specimen can then as a result be further easily examined. One of the methods employed will rapidly produce a specimen sample for quick diagnostic review. A second method employed by the present invention will provide a permanent sample for future review.

Problems solved by technology

However, these techniques, as presently known, are not with out fault as they are often time consuming and burdensome.

While cytology is cheaper and quicker to perform and get diagnostic results than histology methods, the samples produced provide less information and are often easily perishable.

While the protocol is fast, the results are not permanent.

However, this permanent protocol is extremely long and burdensome.

While conventional histology entails protocols to provide an easy to read tissue sample, the process is costly, as materials such as formaldehyde prove to be expensive, and time consuming, results are produced within a long time period.

U.S. Pat. No. 6,459,805 issued to C. Patrick Reynolds on Oct. 1, 2003 shows fluorescence digital imaging microscopy system, yet unlike the present information requires expensive computer microscope technology.