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Cleaning composition and apparatus for removing biofilm and debris from lines and tubing and method therefor

a technology of biofilm and debris removal and cleaning composition, which is applied in the direction of cleaning using liquids, detergent compounding agents, dental surgery, etc., can solve the problems of human infection potential, high danger of infection, and difficult to remove biofilms from tubing

Inactive Publication Date: 2005-02-10
NOVAFLUX INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0010] In accordance with the present invention, a combination of pressurized gas and a suitable aqueous cleaning solution is used to create a turbulent environment on or in a tubing having a biofilm or debris on interior or exterior surfaces, that completely removes the biofilm and debris. A suitable aqueous cleaning solution comprises water and a surfactant. An oxidizing agent and a biocidal agent can also be added. Inert particles that can provide a scrubbing action can also be added advantageously to the aqueous cleaning solution. This aqueous cleaning composition is delivered to the tubing to be cleaned by means of compressed gas, thus making a mixed-phase system, that provides a turbulent flow along the walls of the tubing that aids in loosening the biofilm and debris from the surfaces of the tubing, and aids in flushing the loosened materials from the tubing.
[0011] The invention also includes an apparatus for delivering the above mixed-phase cleaning solution to the interior of small bore tubing and lines and combining it with a gas under pressure. The compressed gas-aqueous cleaning solution combination develops turbulent flow inside the tubing that aids in loosening the biofilm from the interior surfaces of tubing so that it can be readily removed by flushing and rinsing with water.

Problems solved by technology

All of these bacteria have the potential to cause infections in humans, and legionella, which is highly resistant to antibiotics, is of particular concern since infection can be fatal.
If the patient has any open wounds in the mouth, the danger of infection is of course much higher.
Biofilms are very difficult to remove from tubing however.
However, these agents do not readily diffuse into the biofilm which strongly adheres to the tubing walls, and thus removal is generally only partial and the biofilm returns quite rapidly.
Such cleaning is expensive, but necessary.

Method used

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  • Cleaning composition and apparatus for removing biofilm and debris from lines and tubing and method therefor
  • Cleaning composition and apparatus for removing biofilm and debris from lines and tubing and method therefor
  • Cleaning composition and apparatus for removing biofilm and debris from lines and tubing and method therefor

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0068] A portion of dental tubing having a biofilm about one year old on its interior surface is shown in FIG. 2. The biofilm completely covers the interior surface of the tubing and consists of about 200,000 colonies / cm of bacteria.

[0069] A three foot section of the tubing was treated in accordance with the invention using a cleaning solution of 3% aqueous hydrogen peroxide containing 5% by weight of inert particles of calcium carbonate 50-100 microns in size, and surfactants including about 2% of an anionic surfactant, sodium dodecyl sulfate, and about 1% of a nonionic surfactant. The cleaning solution was added to the mixing chamber 28 of FIG. 1 and air was added to the mixing chamber from a 1 HP air compressor regulated at 30-60 psi, the air being continuously pulsed. The amount of air and the pulse rate were regulated to optimize mixing of the ingredients and the turbulence generated inside the tubing. The mixture was passed to the dental tubing to permit turbulent cleaning of...

example 2

[0073] A cleaning solution was made by mixing 50 grams of calcium carbonate particles having a particle size of 10-100 microns into 450 ml of a solution containing 3% by weight of hydrogen peroxide solution, 2% by weight of a surfactant, sodium dodecyl sulfate and 1% of a nonionic surfactant.

[0074] The above mixture was introduced into a used dental tubing 3 feet long and 1.8 mm in diameter having a biofilm thereon, as shown in FIG. 4, together with air supplied by a compressor set between 30 and 60 psi. The cleaning composition was flushed through the tubing for about 3 minutes.

[0075] The tubing was then flushed with 200 ml of distilled water.

[0076] The number of bacterial colonies in the tubing decreased from an initial value of 7.15×105 per linear cm of tubing down to zero after cleaning. The CFU / ml of water flowing in the tube was reduced from an initial value of 3.19×106 to zero after cleaning.

[0077] The SEM photograph of FIG. 5 is an overall view of the interior wall of th...

example 3

[0078] 576 Milliliters of 3% by weight of aqueous hydrogen peroxide solution was admixed with 2% by weight of sodium dodecyl sulfate surfactant. The cleaning reagent was introduced into used dental tubing with air supplied by a compressor set at a pressure of 30 to 60 psi. After treatment for three minutes, the tubing was flushed with 200 ml of water.

[0079] The number of bacterial colonies per cm of tubing after culture decreased from an initial value of 1×105 to zero. The CFU / ml of the water decreased from an initial value of 3.01×106 to zero.

[0080]FIG. 6 is a photograph of the interior wall of the tubing prior to treatment. FIG. 7 is a photograph of the interior wall of the tubing after treatment.

[0081] Thus in the presence of hydrogen peroxide, surfactant and air under a preselected pressure, complete cleaning was achieved even in the absence of solid particles.

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Abstract

Biofilm and debris can be removed from the interior and exterior surfaces of small bore tubing by passing an aqueous cleaning solution of water, one or more surfactants and preferably a source of hydrogen peroxide, optionally including small inert solid particles, together with a gas under pressure, to create a turbulent flow within the tubing that loosens the biofilm and debris so that they can be flushed from the tubing. When the exterior surfaces of tubing are to be cleaned, the tubing is inserted in a sleeve fitted with an adaptor that provides a pressure-tight seal between the tubing and the sleeve.

Description

[0001] This invention relates to a mixed-phase cleaning solution and method for removing bacteria with pathogenic potential and other microorganisms, debris, tissue, food particles and the like from lines and tubing, and to an apparatus that delivers the mixed-phase cleaning solution under pressure to the lines and tubing to be cleaned. BACKGROUND OF THE INVENTION [0002] Small bore tubing that delivers ordinary running water, purified or not, to fixtures such as dental units, develop bacterial and fungal growth from the water on their interior surfaces, as is well known. Bacteria present in the water strongly adhere to tubing surfaces and then grow laterally, forming what is known as biofilm. Biofilm is apparent to the touch as a slimy film which has been analyzed and found to be bacterial growth. [0003] Several prior art workers have identified various bacteria included in these biofilms, which include several bacteria having pathogenic potential, such as Flavobacterium, Moraxella,...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61C17/00A61C1/00A61L2/18A61L2/22A61M1/16B01D65/02B01D65/06B08B9/02B08B9/032C11D3/02C11D3/14C11D3/39C11D3/395C11D3/48C11D11/00C23G5/00
CPCA61C1/0076A61M1/1682A61L2/22A61L2202/24A61M1/169B01D65/02B01D65/022B01D2321/168B01D2321/185B08B9/0326B08B9/0327B08B2209/022C11D3/14C11D3/3947C11D3/3956C11D3/48C11D11/0041C23G5/00A61L2/186C11D2111/20
Inventor LABIB, MOHAMED EMAMLAI, CHING-YUE
Owner NOVAFLUX INC
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