Method for preparing chloromycetin artificial antigen
A technology of artificial antigen and chloramphenicol, which is applied in the field of preparation of chloramphenicol artificial antigen, can solve the problems of difficult antibiotic residue, high price, cumbersome operation, etc., and achieve the effect of low cost, simple operation and rapid detection
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Embodiment 1
[0012] A preparation method of chloramphenicol artificial antigen is characterized in that the following steps are adopted:
[0013] A) Weigh chloramphenicol and succinic anhydride at a weight ratio of 1:0.3 and add them to anhydrous pyridine successively. Medium water bath at 60°C for 24h (a large amount of preparation requires a water bath at 60°C for 24h in a reflux device) to generate carboxyl-containing chloramphenicol derivatives;
[0014] B) Place the above-mentioned chloramphenicol derivatives in a centrifuge tube, add 5 times the amount of ice-cold 5N hydrochloric acid of the original reaction volume of chloramphenicol, and rotate the centrifuge tube at a speed of 1000 rpm for 10 minutes to separate out carboxyl-containing chloramphenicol For the red product, centrifuge and wash the precipitate twice with 2 times the volume of 5N hydrochloric acid ice-cold hydrochloric acid solution to obtain a purple-red precipitate;
[0015] C) adding concentration to the precipita...
Embodiment 2
[0018] A preparation method of chloramphenicol artificial antigen is characterized in that the following steps are adopted:
[0019] A) Weigh chloramphenicol and succinic anhydride respectively in a weight ratio of 1:0.5, and add them to anhydrous pyridine successively. Medium water bath at 60°C for 6h (a large amount of preparation requires a water bath at 60°C for 6h in a reflux device) to generate carboxyl-containing chloramphenicol derivatives;
[0020] B) Place the above-mentioned chloramphenicol derivatives in a centrifuge tube, add 10 times the amount of ice-cold 1N hydrochloric acid of the original reaction volume of chloramphenicol, and rotate the centrifuge tube at a speed of 1000 rpm for 10 minutes to separate out carboxyl-containing chloramphenicol For the red product, centrifuge and wash the precipitate 3 times with 10 times the volume of 1N hydrochloric acid ice-cold hydrochloric acid solution to obtain a purple-red precipitate;
[0021] C) adding a concentratio...
Embodiment 3
[0024] A preparation method of chloramphenicol artificial antigen is characterized in that the following steps are adopted:
[0025] A) Weigh chloramphenicol and succinic anhydride at a weight ratio of 1:0.4 and add them to anhydrous pyridine successively. Medium water bath at 60°C for 15h (a large amount of preparation requires a water bath at 60°C for 15h in a reflux device) to generate carboxyl-containing chloramphenicol derivatives;
[0026] B) Place the above-mentioned chloramphenicol derivatives in a centrifuge tube, add 7 times the amount of ice-cold 3N hydrochloric acid of the original reaction volume of chloramphenicol, and rotate the centrifuge tube at a speed of 1000 rpm for 10 minutes to separate out carboxyl-containing chloramphenicol For the red product, centrifuge and wash the precipitate 3 times with 7 times the volume of 3N hydrochloric acid ice-cold hydrochloric acid solution to obtain a purple-red precipitate;
[0027] C) adding a concentration of 55% ethan...
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