Assemble preparation method of DNA guided nano granule tree-shape wire
A nanoparticle and nanometal particle technology, which is applied in the field of chemical self-assembly method to manufacture functional devices, can solve the problems of difficult precise control of DNA skeleton structure, difficult regulation of assembly, difficult regulation, etc.
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Embodiment 1
[0017] A method for preparing a DNA-guided nanoparticle-assembled dendritic wire, comprising the following steps: (1) placing an aqueous solution of gold particles with an average particle diameter of 10 nanometers and a concentration of 200 nM with a volume ratio of 400:1 in a concentration of 50μM of the 5' end sulfhydryl modified deoxyribonucleic acid sequence SH-(CH 2 ) 6 -T 8 solution (T is thymidine), stirred for 4 hours, so that the surface of gold nanoparticles formed a DNA modification layer by interacting with sulfhydryl groups; (2) adding dimethyl sulfoxide to make dimethyl sulfoxide in the solution The concentration is 1.5mM, adding tris-HCl buffer solution to make the pH value 6; and adding K + and Mg 2+ so that the solution contains K + The concentration is 50mM, Mg 2+ The concentration is 0.8mM. (3) Add another DNA sequence A 8 G 10 (A is adenine deoxynucleotide, G is guanine deoxynucleotide), the concentration of another DNA sequence in the solution is ...
Embodiment 2
[0019] A method for preparing a DNA-guided nanoparticle-assembled dendritic wire, comprising the following steps: (1) placing an aqueous solution of gold particles with an average particle diameter of 10 nanometers with a volume ratio of 100:1 and a concentration of 5 nM in a concentration of 0.3mM 5' end sulfhydryl modified deoxyribonucleic acid sequence SH-(CH 2 ) 4 -T 20 In the solution, stir for 6 hours, make the surface of the gold nanoparticles form a DNA modification layer by interacting with mercapto groups; (2) add trimethoxymercaptopropylsilane, so that the concentration of trimethoxymercaptopropylsilane in the solution is 3.5mM, Add tris-sodium borate buffer solution to make the pH value 8; and add Na + and Mg 2+ so that the solution contains Na + The concentration is 30mM, Mg 2+ The concentration was 1.0 mM. (3) Add another DNA sequence A 6 G 13 The concentration of the other DNA sequence in the solution is 0.1 mM, the temperature is raised to 90° C., natur...
Embodiment 3
[0021] A method for preparing a DNA-guided nanoparticle-assembled dendritic wire, consisting of the following steps: (1) placing an aqueous solution of silver particles with an average particle diameter of 10 nanometers at a concentration of 5 nM with a volume ratio of 600:1 0.1mM 5' end sulfhydryl modified deoxyribonucleic acid sequence SH-(CH 2 ) 10 -T 25 In the solution, stir for 2 hours, make the surface of nano-silver particles form a DNA modification layer by reacting with mercapto groups; (2) add trichloroethyl phosphate, make the concentration of trichloroethyl phosphate in the solution be 1.5mM, add trichloroethyl phosphate hydroxymethylaminomethane-HCl buffer solution to make the pH value 6; and add K + and Mg 2+ so that the solution contains K + The concentration is 70mM, Mg 2+ The concentration is 0.5 mM. (3) Add another DNA sequence A 10 G 8 , the concentration of the other DNA sequence in the solution is 30 μM, the temperature is raised to 95° C., natural...
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