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Effective liquid phase chromatography for measuring alkali content of dita leaves

A technology of high performance liquid chromatography and saffron leaf alkali, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as defects, difficult quality analysis of Taiwan leaf medicinal materials and their preparations, etc.

Active Publication Date: 2007-06-06
YUNNAN PHYTOPHARML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] After the inventor's systematic research, the results show that the main technical conditions determined in the above patent application have serious defects, and it is difficult to actually apply it to the quality analysis of the medicinal material and its preparations.

Method used

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  • Effective liquid phase chromatography for measuring alkali content of dita leaves
  • Effective liquid phase chromatography for measuring alkali content of dita leaves
  • Effective liquid phase chromatography for measuring alkali content of dita leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Embodiment 1: Determination of the content of phylloxine in the leaves of Dengtai

[0103] Take the leaves of the lampstand, crush them, pass through a 60-mesh sieve, weigh 2g precisely, put them in a 100ml round bottom flask, add 50ml of methanol accurately, weigh, extract in a water bath under reflux for 1 hour, let it cool, make up the lost weight with methanol, and filter , that is, the test solution. Take an appropriate amount of japonica, accurately weighed, and dissolve with HPLC mobile phase to make a reference solution containing 50 μg per 1 ml.

[0104] According to the method (Table 6-Experiment 8) determined in the above-mentioned research, accurately draw the reference substance solution and the test solution, inject them into a liquid chromatograph, measure, and get final product. See Table 17 for the results of determination of alkaloid content in the leaves of Dracaena japonica from different places of origin.

[0105] Sample lot number

Embodiment 2

[0106] Embodiment 2: Determination of the content of phylloxine in the root of the lampstand tree

[0107] The method is the same as that in Example 1, and the medicinal part to be measured is the root of D. chinensis. The results of the determination of the content of alkaloids in the roots of D. chinensis from different places are shown in Table 18.

[0108] Sample lot number

Embodiment 3

[0109] Embodiment 3: Determination of the content of Dracaena foliage in the lampstand branch

[0110] The method is the same as that in Example 1, and the medicinal part to be measured is the branch of the lampstand tree, and the results of the determination of the content of alkaloids in the stems of the lampstand tree from different origins are shown in Table 19.

[0111] Sample lot number

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Abstract

The HPLC measures the picrinine content. The condition of HPLC is: the bulking agent of the chromatographic column is the carbon 18 alkyl bonding with the silica gel; the mobile phase is methanol or the acetonitrile mixed with the triethylamine or the diethylamine and the detection wave is 287nm. The invention can be used to detect the picrinine content not only in the Cornus controversa Hemsl (root, stem, skin and leaf), pharmaceutics (particle, sheet, capsule, soft extracts and peroral liquid) but also in other material. The method is quick, accurate and special. The all components in Cornus controversa Hemsl and pharmaceutics can flow out the chromatographic column within 40min.

Description

technical field [0001] The invention relates to a chromatographic analysis method, in particular to a high-performance liquid chromatography (HPLC) method for determining the content of picrinine, and belongs to the technical field of drug analysis. Background technique [0002] Alstonia Scholaris (L.) R.Bro., also known as duck foot tree, sugar gum tree, etc., is a plant of the genus Apocynaceae. It is a tall and long green tree. It is born in hills and mountains below 850 meters above sea level. Sparse forest. Domestically distributed in Yunnan, Guangxi, Fujian, etc., abroad in India, Sri Lanka, Myanmar, Thailand, Vietnam, Philippines, etc., rich in resources. [0003] In Yunnan Province, the lampstand tree is a natural medicine used by many ethnic groups, and its roots, stems, bark and leaves can be used as medicine. The Han people call it "Lifei San", which is an effective drug for treating respiratory diseases; the Dai people call it "Maidanbie", which is mainly used ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/26
Inventor 郭文孙赟朱丽萍龚云麒
Owner YUNNAN PHYTOPHARML
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