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Lignin synthesis related protein and its coding gene and uses

A technology of related proteins and coding genes, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of isolation and identification of genes without lignin, and achieve the effect of improving value

Active Publication Date: 2007-05-23
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rice is the food crop with the largest planting area in my country, and its straw can be used for pulping and feed, but so far no lignin-related genes have been isolated and identified in rice

Method used

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  • Lignin synthesis related protein and its coding gene and uses
  • Lignin synthesis related protein and its coding gene and uses
  • Lignin synthesis related protein and its coding gene and uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. Obtainment of lignin synthesis-related protein GH2 and its coding gene

[0041] 1. Phenotype analysis of rice golden glume mutant gh2

[0042] The rice golden glume mutant gh2 is a near allelic line of ZF802. Compared with the control ZF802, the phenotype of golden glumes is brown-red on the leaf sheaths and stems from the jointing stage, and the glumes are also brown-red at the heading stage, and the brown-red color gradually changes as the rice matures. Pale, turning brownish-yellow at maturity. The mutant phenotype does not affect the normal growth and development of rice under normal cultivation conditions (Fig. 1). In Figure 1, a is the ear of the control ZF802, green; b is the ear of the golden glume mutant gh-2, reddish brown; c is the stem of the control ZF802, green; d is the stem of the golden glume mutant gh2, reddish brown; e, the seeds of the control ZF802 are light yellow; f, the seeds of the golden glume mutant gh2 are brown.

[0043] 2. An...

Embodiment 2

[0069] Embodiment 2, Complementation experiment of the gh2 phenotype of the golden glume mutant

[0070] 1. Construction of the expression vector pCGH containing the genomic gene coding sequence of GH2 whose 3465th deoxynucleotide at the 5' end of sequence 3 is G

[0071] BAC OJ1145_F01 (purchased from the Institute of Genetics and Development, Chinese Academy of Sciences) was digested with BamH I and Hind III to obtain 2,397 bases upstream of the start codon ATG and 2460 bases after the stop codon TGA of GH2 The DNA fragment of the full-length sequence was cloned into pCAMBIA1300 (CAMBIA, Canberra, Australia) between the BamH I and Hind III recognition sites, and the GH2 containing the 3465th deoxynucleotide at the 5' end of sequence 3 was constructed to be G. The coding sequence of the genomic gene (3585bp in total) was expressed in the vector pCGH (Fig. 4).

[0072] 2. Construction of the expression vector pCGHS containing the 3465th position from the 5' end of sequence 3 ...

Embodiment 3

[0081] Embodiment 3, classic hybridization technique utilizes GH2 gene

[0082] The male parent of the golden glume mutant gh2 was crossed with the recipient plant ZF802 as the female parent, and then the recipient plant ZF802 was repeatedly backcrossed 10 times to transfer the phenotype of the golden glume mutant gh2 to the recipient plant, and the GH2 The genetically modified plant has the phenotype of the golden glia mutant gh2 as shown in FIG. 1 .

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Abstract

The invention discloses a lignin-related protein as well as its gene encoding and application. The lignin-associated protein has one of the following amino acid residues sequences: (1) SEQ ID No. : 2 in the sequence table, and (2) lignin-related protein after SEQ ID No. : 2 one or several amino acid residues replaced and / or deleted and / or added in the sequence table. The lignin-related protein gene can regulate the content and composition of rice lignin without influences on plant growth and development, and improve the values as feed and industrial raw material of paper. In addition, the lignin-related protein gene also has important theoretical value in revealing the mechanism of plant lignin biosynthesis.

Description

technical field [0001] The invention relates to a lignin synthesis-related protein, its coding gene and application in the field of plant genetic engineering. Background technique [0002] Lignin is a kind of polyphenolic biopolymer whose content in plant cell wall is second only to cellulose. Lignin can improve the strength of cell wall by cross-linking with carbohydrates such as cellulose hemicellulose, and improve the resistance of plants to diseases and insect pests. Lignin participates in the formation of conduits and sieves, so that water and solutes can be transported through the vascular system (MalcolmM. Campbell and Ronald R. Sederoff. Variation in lignin content and composition. Plant physiol, 1996, 110: 3-13). Lignin is mainly derived from complex racemic aromatic heteropolymers of three hydroxycinnamyl alcohol monomers—p-coumaryl M1H, coniferyl M1G, and sinapyl M1S (Wout Boerjan, John Ralph, and Marie Baucher. Lignin biosynthesis. Annu. Rev. Plant Biol. 2003. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N15/82A01H4/00
Inventor 程祝宽钱前张可伟李明
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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