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Rapid test paper bar for testing colloidal gold of 01 group cholera vibrio

A technology for detecting Vibrio cholerae and test strips, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of high technical requirements, expensive equipment, and failure to meet the requirements of diarrhoea and must be tested, and achieves clear, easy-to-recognize and timely results. The effect of controlling the epidemic and saving manpower and material resources

Inactive Publication Date: 2007-05-16
BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the traditional detection method of Vibrio cholerae O1 group is direct isolation and culture, isolation and culture after enrichment, etc. The operation is complicated and requires professional and technical personnel to carry out in a professional laboratory. It takes 16-24 hours and cannot handle a large number of suspicious specimens. Therefore, For the detection of Vibrio cholerae O1 group, it is far from meeting the requirements of every diarrhea, and at the same time, the detection time cannot meet the purpose of rapid screening.
Such as PCR, latex agglutination test and monoclonal antibody diazo-based latex test (DPRC), although they can diagnose Vibrio cholerae within a few hours, with high specificity and sensitivity, but at the same time they have various Different kinds of defects, such as: high technical requirements, relatively expensive equipment, etc.

Method used

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  • Rapid test paper bar for testing colloidal gold of 01 group cholera vibrio

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 O1 group Vibrio cholerae colloidal gold rapid detection test strip (see Figure 1)

[0030] The reaction support is 6.5cm×0.4cm PCV plate; the absorbent pad is 2cm×0.4cm oil filter paper; 1.8cm×0.4cm nitrocellulose membrane is coated with anti-mouse IgG and monoclonal antibody against O1 Vibrio cholerae A antigen in sequence Or polyclonal antibody, containing 0.4cm×0.4cm colloidal gold-labeled monoclonal antibody glass fiber membrane of O1 group Vibrio cholerae A antigen; the protective film of gold-labeled antibody is a 2.7cm×0.4cm polyester film; that is, O1 Vibrio cholerae colloidal gold rapid detection test strip.

Embodiment 2

[0031] Embodiment 2: O1 group Vibrio cholerae colloidal gold rapid detection test strip (see Figure 1)

[0032] The reaction support is 6.5cm×0.4cm PCV plate; the absorbent pad is 2cm×0.4cm oil filter paper; 1.8cm×0.4cm nitrocellulose membrane is coated with anti-mouse IgG and monoclonal antibody against O1 Vibrio cholerae A antigen in sequence Or polyclonal antibody, containing 0.4cm×0.4cm colloidal gold-labeled monoclonal antibody glass fiber membrane of O1 group Vibrio cholerae A antigen; gold-labeled antibody protective film is 2.7cm×0.4cm glass fiber; that is, O1 group is formed Vibrio cholerae colloidal gold rapid test strips.

Embodiment 3

[0033] Example 3 O1 Vibrio cholerae colloidal gold rapid detection test strip (see Figure 1)

[0034] The reaction support is 6.5cm×0.4cm PCV plate; the absorbent pad is 2cm×0.4cm oil filter paper; 1.8cm×0.4cm nitrocellulose membrane is coated with anti-mouse IgG and monoclonal antibody against O1 Vibrio cholerae A antigen in sequence Or polyclonal antibody, monoclonal antibody glass fiber membrane containing 0.4cm×0.4cm colloidal gold-labeled Vibrio cholerae A antigen of group O1; the protective film of gold-labeled antibody is filter paper fiber of 2.7cm×0.4cm; that is, O1 group is formed Vibrio cholerae colloidal gold rapid test strips.

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Abstract

This invention provides one rapid test bar for 01 cholera vibrio antigen, which covers 01 cholera vibrio An antigen single clone antibody or multiple clone antibody and quality IgG on NC film and combines glue gold label single clone antigen and applies film analysis double antibody clamper and tests the cholera vibrio antigen.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a test strip for O1 group Vibrio cholerae antigen detection and an application thereof. Background technique [0002] Cholera is an acute infectious disease caused by Vibrio cholerae infecting organisms, and its typical feature is massive loss of body fluid and electrolytes caused by severe diarrhea. Vibrio cholerae can be divided into more than 200 serogroups according to the specificity of its bacterial antigens, of which the most common and most infectious is the O1 group. [0003] At present, the traditional detection method of Vibrio cholerae O1 group is direct isolation and culture, isolation and culture after enrichment, etc. The operation is complicated and requires professional and technical personnel to carry out in a professional laboratory. It takes 16-24 hours and cannot handle a large number of suspicious specimens. Therefore, For the detection of Vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/558G01N33/544
CPCY02A50/30
Inventor 刘明
Owner BEIJING ZHUANGDI HAOHE BIOMEDICINE SCI & TECH
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