Process for preparing copolyester soybean esterase

Abstract

The invention relates to the preparing method of soybean exterase which can be used in the detection of the vegetable and fruits' pesticide residue. The producing procedure comprises the following steps: choosing the high-quality soybean, pulverizing, extracting with phosphate buffer, differential centrifugation separating, salt precipitating by ammonium, purifying by ion exchange chromatography and freeze-drying. The invention has a simple and steady producing technology. It also costs less than the method of extracting pure acetylcholine exterase products. The purified product's sensitivity is more than 19 times as the sensitivity of the enzyme crude extracts. The LDC of monocrotophos, methamidophos, alkron, durshan, dantox, carbofos and chlorofos has reached 0.03125ppm. The LDC of dursan and methidathion is one thirty-second of their MRL, while the LDC of diethion, alkron and Phosalone is one sixteenth of their MRL. The invention can fill the needs of fast detection of phosphate pesticide; it can also be widely used as the enzyme labeling marker in different methods of detecting the pesticide residue.

Description

technical field [0001] The invention relates to a method for detecting pesticide residues, in particular to a method for preparing soybean esterase for rapid detection of organic phosphorus and carbamate pesticide residues in fruits and vegetables. Background technique [0002] At present, organophosphorus pesticides and carbamate pesticides are still widely used in agricultural production, and they are very easy to cause residues in agricultural products, especially in fruits and vegetables, which are more prominent, causing harm to human health. Therefore, pesticide residues are important detection and control indicators for food quality and safety. In recent years, there have been many studies on rapid detection methods for pesticide residues. Among them, "enzyme inhibition-spectrophotometry" has been widely used because of its sensitivity, simplicity and speed, and has been listed as a national recommended standard (GB / T5009.199- 2003). The enzy...

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Problems solved by technology

Existing plant esterase sheet preparation report at present, as the patent office disclosed application number is 99114268.3 " the plant esterase sheet of rapid assay organophosphorus pesticide " patent of invention, and it is directly to use flour as enzyme source material, but prepared The sensitivity and minimum detection limit of esterase tablets to detect residual pesticides are far from the safety limit requirements stipulated in the national standard

In addition, there is no research report on the preparation method of plant esterase pure enzyme products