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Polypeptides of alicyclibacillus

A technology of bacillus and alicyclic acid, applied in the direction of peptides, peptides, bacteria, etc.

Inactive Publication Date: 2007-03-14
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] A disadvantage of genome sequencing is that the vast majority of sequences obtained encode non-secreted proteins

Method used

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  • Polypeptides of alicyclibacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0386] Example 1: Identification of functional polypeptides secreted by Alicyclobacillus DSM 15716

[0387] Construction of a genomic library

[0388] The chromosomal DNA of Alicyclobacillus DSM 15716 was prepared by using standard molecular biology techniques (Ausuble et al., 1995 "Current protocols in molecular biology", published by John Wiley and Sons). The prepared DNA was partially digested with Sau3A and separated on an agarose gel. Elute, precipitate and resuspend the 3 to 8 kilobase fragments in the appropriate buffer.

[0389] By using Stratagene ZAP Express TM Pre-digested vector kit and StratageneZAP Express TM Pre-digested Gigapack  The cloning kit (Bam HI pre-digested) (Stratagene Inc., USA) was used to prepare a genomic library according to the manufacturer's instructions / recommendations. The produced lambda ZAP contains 38,000 pfu, and 10,000 of which are selected for large-scale digestion. The 70,000 E. coli colonies produced were mixed and plasmids were prepared...

Embodiment 2

[0409] Example 2: Determining function by homology

[0410] Annotate the functions of the polypeptides SEQ ID NO: 26 to SEQ ID NO: 50 by comparing with gene or polypeptide sequences with known functions. Compare the polypeptides of the invention with a list of the most relevant sequences from public and internal contig databases. The BLASTX 2.Oa19MP-WashU [July 14, 1998] program was then used to compare the contigs (from which SEQ ID NO: 26 to SEQ ID NO: 50 are derived) and sequences that can be obtained from standard public DNA and protein sequence databases. Careful analysis of the sequence alignment of SEQ ID NO: 26 to SEQ ID NO: 40 with their most relevant functional known sequences from other databases makes it possible to predict the function of these polypeptides based on the degree of amino acid identity. Even when the total amino acid identity is 40% (usually difficult to make good predictions), we can predict SEQ ID NO: 26 to SEQ ID NO by carefully analyzing and interpre...

Embodiment 3

[0411] Example 3: Preparation of the polypeptides of SEQ ID NO: 26 to SEQ ID NO: 50

[0412] To prepare the polypeptides of SEQ ID NO: 26 to SEQ ID NO: 50, the DNA encoding the open reading frame is combined with a suitable host strain (such as Escherichia coli, Bacillus subtilis, Bacillus licheniformis or Bacillus clausii or lipid The promoter, ribosome binding site, and terminator of the gene expressed in Bacillus circulans derivative) are fused to express the genes encoding these polypeptides contained in SEQ ID NO: 1 to SEQ ID NO: 25. The promoter can be an inducible promoter or a constitutive promoter. Any signal sequence of SEQ ID NO: 26 to SEQ ID NO: 50 can be exchanged with an appropriate signal peptide of another bacterium. The expression construct can be a plasmid or part of linear DNA. It can be integrated into the chromosome of the host strain by recombination or it can be present as a plasmid in the host cell. Then, the transformed cells carrying the gene of interest ...

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Abstract

The present invention relates to Isolated mature functional polypeptide which is at least 90% identical to and exhibits the same function of a corresponding secreted polypeptide obtainable from the bacterium Alicyclobacillus sp. deposited under accession number DSM 15716 are disclosed.

Description

Technical field [0001] The present invention relates to functional and effective polypeptides encoded by polynucleotides contained in the genome of the bacterium Alicyclobacillus bacillus deposited under the deposit number DSM 15716. The present invention also relates to polynucleotides encoding these polypeptides or promoting their expression, constructs of these polynucleotides, and methods for preparing the polypeptides. The present invention also relates to a composition comprising the polypeptide and polynucleotide and the use of the polypeptide. The present invention also relates to Alicyclobacillus bacteria deposited under the deposit number DSM 15716. Background technique [0002] Some enzymes derived from Alicyclobacillus acidocaldarius are known, such as Matzke et al.; Genecloning, nucleotide sequence and biochemical properties of a cytoplasmiccyclomaltodextrinase (neopullulanase) from Alicyclobacillus acidocaldarius ATCC 2700; classification of a group of enzymes, subm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N9/42C12N9/52C12N9/24C12N9/90C12N15/53C12N15/55C12N15/61C07K14/32C12N9/16C12N1/21
Inventor R·惠尔廷S·F·拉森P·R·奥斯特伽德
Owner NOVOZYMES AS
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