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Industrial high power breeding method for entomopathogenic nematode alive body

An entomopathogenic nematode and industrialized technology, applied in the direction of protozoa, can solve the problem of reduced pathogenicity

Inactive Publication Date: 2007-03-14
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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Problems solved by technology

Although the nematodes obtained by the traditional white trap method have high infectivity, their scale is far from meeting the needs of today's market. Guangdong Entomological Research Institute Han Richou adopts the method of artificial in vitro propagation. 1, 20 liters to the scale of Biosys Company to produce 15,000 liters of Steiner's nematodes and 7,500 liters of Heterobacter nematodes. Although the output of nematodes is high, its pathogenicity has decreased

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Embodiment Construction

[0019] The present invention is the main content of the subject frontier field project of the Chinese Academy of Sciences: "Using Pathogenic Nematodes to Control Soybean Underground Pests and Soybean Cyst Nematode rDNA-ITS-RFLP Research" (No. 20020101). From January 2002 to December 2005, we carried out factory experimental production according to the above steps at the Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, and obtained the success of the best high-multiplication method for live entomopathogenic nematodes, which can solve the problem of nematodes. There are few difficult problems that cannot be applied to production practice. After testing, the effect of preventing and controlling underground pests has reached more than 80%, which has not only obtained economic benefits, but also obtained ecological benefits.

[0020] The materials used in the invention are widely distributed, and the price is cheap, which can reduce the cost. ...

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Abstract

The present invention belongs to the field of agricultural production technology, and is especially industrial high power living body breeding method of insect pathogen nematode. The present invention adopts living body breeding, rather than in vitro breeding, and has obviously raised agricultural pest invading power, high yield and efficiency, low cost, high quality and obviously raised pest preventing and controlling effect. The present invention may be used widely in preventing and controlling pest of potted crop, economic crop, vegetable, pasture, etc.

Description

technical field [0001] The invention belongs to the technical field of agricultural production. It is a high-multiplication method for the living body of industrialized entomopathogenic nematodes. technical background [0002] At present, chemical agents are mainly used in the control of underground pests and hidden pests, but this method is easy to cause poisoning of humans and animals, and also pollutes the environment. Moreover, the cost of repeated control is high, and the pests are prone to develop resistance. With the improvement of people's food safety requirements and the consideration of the potential crisis that chemical agents may bring to the ecological environment, it is urgent to screen biological control agents that can replace chemical agents. [0003] Today, Bacillus thuringiensis, Beauveria brongniatii, Metarhizium anisopliae, etc. have been screened out as biological control agents, but the effect of controlling some underground pests and hidden pests is...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/10
Inventor 李春杰许艳丽王义
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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