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Application of BcSfp1 gene in prevention and treatment of plant gray mold and improvement of disease resistance

A botrytis cinerea and gene technology, applied in the field of microbial genetic engineering, can solve problems such as unreported research, and achieve the effects of improving disease resistance and stress resistance, and enhancing pathogenicity

Pending Publication Date: 2022-08-05
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Transglutaminase is widely used in many fields such as food, industry, agriculture, biomedicine, textile and cosmetics, but there is no report on transglutaminase in Botrytis cinerea

Method used

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  • Application of BcSfp1 gene in prevention and treatment of plant gray mold and improvement of disease resistance
  • Application of BcSfp1 gene in prevention and treatment of plant gray mold and improvement of disease resistance
  • Application of BcSfp1 gene in prevention and treatment of plant gray mold and improvement of disease resistance

Examples

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Effect test

Embodiment 1

[0051] Example 1. Correlation analysis of BcSfp1 gene

[0052] The open reading frame of BcSfp1 gene of Botrytis cinerea consists of 793 nucleotides, including 3 exons, and the total length of the coding region is 681 nucleotides. The encoded protein product consists of 226 amino acids. The BcSfp1 gene was predicted and analyzed in the Botrytis cinerea genome network http: / / fungi.ensembl.org / Botrytis_cinerea. The BcSfp1 gene number is BCIN01G01260. The NCBI protein blast results show that the amino acid sequence contains five functional domains, namely GATase1_PfpI_2 ( 6-197), GlxA (2-196), DJ-1_PfpI (64-183), ftrA (122-183) and not_thiJ (64-178), the predicted maps such as figure 1 shown.

[0053] 2. Knockout and genetic complementation of BcSfp1 gene

[0054] 1) Knockout vector construction

[0055] Primers were designed by DNAMAN and synthesized by Shanghai Sangon Biotechnology Co., Ltd.

[0056] Using primers BcSfp1-UP-F (5'-GGCGGCTCGAGACAGCGAATGGTGTGATAATCAG-3') and ...

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Abstract

The invention provides application of a BcSpd1 gene in prevention and treatment of plant gray mold and improvement of disease resistance, a nucleotide sequence of the BcSpd1 gene is shown as SEQ ID NO.1, an amino acid sequence of coded protein is shown as SEQ ID NO.2, expression of the BcSfp1 gene and a protein product coded by the BcSfp1 gene are used as target factors to be applied to design and screening of plant anti-gray mold agents, and the application of the BcSfp1 gene and the protein product coded by the BcSfp1 gene in prevention and treatment of the plant gray mold is wide. A compound capable of enhancing gene expression and protein expression, modification and positioning can be screened, plant disease resistance can be effectively improved, gray mold can be controlled, and development of novel bactericides and immune inducers is facilitated.

Description

technical field [0001] The invention belongs to the technical field of microbial genetic engineering, and in particular relates to the application of the botrytis cinerea BcSfp1 gene in the control and disease resistance improvement of botrytis cinerea in plants. Background technique [0002] Botrytis cinerea, commonly known as Botrytis cinerea, is the causative bacterium of Botrytis cinerea, and has a phenotype of Botryotinia fuckeliana (de Bary) Whetzal, which is the phylum Botrytis cinerea A member of the genus Bacteria; asexual form is Botrytis cinerea Pers, a member of the genus Botrytis asexual fungi. Botrytis cinerea can infect more than 1,400 species of plants, including Solanaceae, Cucurbitaceae, Rosaceae, and Leguminosae. Botrytis cinerea is a typical low temperature and high humidity disease. It occurs seriously when the temperature is between 20 °C and 23 °C and the relative humidity is above 90%. It can occur from the seedling stage to the fruiting stage. Infe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N9/10C12N15/82A01H5/00A01H6/20C12R1/645
CPCC12N9/104C12N15/8282C12N15/8279C12Y203/02001C12Y203/02002
Inventor 刘守安陈虎臣王明阳李雯玲
Owner JILIN UNIV
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