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Method for detecting genotype of gene CYP1A2 associated with medicament metabolism ability and liver cancer susceptibility

A technique for genotype and liver cancer, applied in the direction of biochemical equipment and methods, microbial measurement/testing, etc., can solve problems such as inability to fully explain individual differences in CYP1A2 activity

Inactive Publication Date: 2007-02-07
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these polymorphisms cannot fully explain the individual differences in CYP1A2 activity in the population
However, there are no research reports on the association of AFB1 metabolic activating enzyme gene polymorphisms with genetic susceptibility to liver cancer

Method used

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  • Method for detecting genotype of gene CYP1A2 associated with medicament metabolism ability and liver cancer susceptibility
  • Method for detecting genotype of gene CYP1A2 associated with medicament metabolism ability and liver cancer susceptibility
  • Method for detecting genotype of gene CYP1A2 associated with medicament metabolism ability and liver cancer susceptibility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] In this example, a set of primer pairs was designed and synthesized, and on the basis of the primer pairs, a method for detecting three genes CYP1A2 G-3860A, G-3113A and T5347C associated with drug metabolism ability and susceptibility to liver cancer was designed. SNP locus genotype method.

[0043] 1. Genomic DNA extraction

[0044] Genomic DNA of peripheral blood leukocytes was extracted by Miller's modified salting-out method, which is conventional in the field.

[0045] 1) Take sodium citrate or EDTA-Na 2 5ml of anticoagulated whole blood (try not to use heparin for anticoagulation), put in a 50ml capped centrifuge tube;

[0046] 2) Add 3-5 times the volume of cold distilled water, invert and mix repeatedly, place in ice for 5 minutes, and centrifuge at 2000rpm at 4°C for 20 minutes;

[0047]3) Slowly decant the supernatant, add ice-precooled 0.1% Triton-X100 (same volume as above) to the precipitate, gently mix the precipitate, centrifuge as above, and discard ...

Embodiment 2

[0059] This embodiment uses the method established in Example 1 to genotype the CYP1A2G-3860A, G-3113A and T5347C loci in 419 healthy Chinese population, and observe the genotypes of the three loci. Relationship between plasma caffeine metabolic ratio or CYP1A2 activity in vivo.

[0060] 1. Test object:

[0061] 419 healthy subjects (237 males, 182 females) aged 18-33 (average age 20±2 years old) participated in this experiment. All the subjects are students of Central South University. All subjects were non-smoking individuals, and had not consumed coffee, tea, Coca-Cola, chocolate or other caffeinated beverages within 1 week before the detection of caffeine metabolic rate. At the same time, all the subjects did not take any drugs, including contraceptives, within 1 week before the test. The entire experimental process was carried out in accordance with the National Human Genome Research Ethics Guidelines, and informed consent was obtained from all subjects.

[0062] 2. D...

Embodiment 3

[0069] In this example, the method established in Example 1 was used to determine the genotypes of CYP1A2 G-3860A, G-3113A and T5347C loci in 431 cases of liver cancer patients and 550 cases of control DNA samples from Fusui County, Guangxi Zhuang Autonomous Region and its surrounding counties and cities. Genotypes and haplotype combinations were analyzed.

[0070] 1. Test object:

[0071] From June 2003 to December 2004, we collected a total of 431 liver cancer specimens from Fusui County Liver Cancer Institute and Guangxi Zhuang Autonomous Region Cancer Hospital (about 40 kilometers away from Fusui County). Most of the cases in the Cancer Hospital of Guangxi Zhuang Autonomous Region come from Fusui County and surrounding counties and cities. The diagnosis basis of liver cancer cases included: 89 cases (20.6%) were confirmed by histopathology, 247 cases (57.3%) were positive in imaging diagnosis (B-ultrasound examination and / or CT scan) accompanied by elevated serum alpha-fe...

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Abstract

the invention discloses a gene CYP1A2 genotypic and detecting method and relative genotypic agent box and application, which provides molecular criterion for metabolic dose.

Description

technical field [0001] The invention relates to individual differences in drug metabolism ability, individual differences in susceptibility to liver cancer in high-incidence areas of liver cancer and a genetic detection method thereof. Specifically, the present invention relates to a method for detecting the genotype of the gene CYP1A2 related to drug metabolism ability and liver cancer susceptibility; the invention further relates to a detection kit for the above-mentioned gene CYP1A2 genotype associated with drug metabolism ability and liver cancer susceptibility, and The application of the kit; the present invention identifies the CYP1A2 genotype that leads to low CYP1A2 enzyme activity in vivo, thereby involving the application in clinical individualized medicine; the present invention also determines that CYP1A2 is a new type of susceptibility gene for liver cancer in high-incidence areas of liver cancer, This involves early screening and chemoprevention of individuals at...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 贺福初陈小平程泽能汪海健王黎青智联腾周钢桥谢伟敏朱云平
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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