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Method of realizing microorganism in situ separation and fermentation employing inorganic film

An in-situ separation, inorganic membrane technology, applied in specific-purpose bioreactors/fermenters, biochemical equipment and methods, microorganisms, etc., can solve problems such as inability to separate target products or metabolic by-products, and achieve higher yields , Improve the growth rate, the effect of high-density culture

Inactive Publication Date: 2007-02-07
天津市新星兽药厂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since fed-batch fermentation cannot separate the target products or metabolic by-products that inhibit the growth of the bacteria in time, it is difficult for those strains whose fermentation metabolites have a serious inhibitory effect on the bacteria to undergo fed-batch fermentation. Achieve high cell concentration and target product yield

Method used

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  • Method of realizing microorganism in situ separation and fermentation employing inorganic film

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Effect test

Embodiment 1

[0025] Example 1: Production of vitamin B by P. shermanii 12 in situ separation fermentation

[0026] Propionic acid, the main primary metabolite in the submerged fermentation process of P.shermanii, has a serious inhibitory effect on bacterial growth, while vitamin B 12 It is the intracellular secondary metabolite of P.shermanii, so timely remove propionic acid from the fermentation system, relieve the inhibitory effect of propionic acid on the bacteria, and achieve high-density culture will greatly increase the vitamin B 12 output.

[0027] The in-situ separation fermentation reactor is mainly composed of a fermenter 3, an inorganic membrane module 4 and 8, a chromatographic column 7, a feeding tank 1, a liquid storage tank 5 and 6, and a peristaltic pump 2, wherein the inorganic membrane module includes two groups, The first group of inorganic membrane modules, i.e. inorganic membrane modules 4, are used to filter metabolites that are unfavorable to the growth of bacteria...

Embodiment 2

[0047] In Situ Isolation and Culture of Bacillus cogulans TQ33

[0048] The lactic acid produced during the fermentation of Bacillus coagulans has a strong inhibitory effect on the growth of the bacteria, so the timely separation of the lactic acid from the fermentation broth will greatly increase the concentration of the bacteria and spores. The 7L fermenter used in the experiment was purchased from Shanghai Gaoji Company, and the inorganic ceramic membrane module was purchased from Anhui Shijie Membrane Engineering Co., Ltd., with a pore size of 0.2 μm. Anion exchange resin D354 was purchased from Hangzhou Zhengguang Resin Co., Ltd.

[0049] 1: culture medium

[0050] (1) Seed medium (g / L)

[0051] Peptone 10.0, yeast extract 10.0, glucose 60.0, MgSO 4 ·7H 2 O 0.5, CaCO 3 10.0. pH7.2-7.4.

[0052] (2) Fermentation medium (g / L)

[0053] Peptone 10.0, yeast extract 10.0, glucose 6.0, MgSO 4 ·7H 2 O 1.0, K 2 HPO 4 2.0, MnSO 4 50mg / L pH 7.0.

[0054] (3) GYP med...

Embodiment 3

[0065] In Situ Separation and Fermentation of Exopolysaccharides Produced by Schizophyllum

[0066] Schizophyllum exopolysaccharide is a water-soluble β-D-glucan, because of its good water solubility and the unique properties of β-(1-3)-D glucose with β-(1-6) branches The active structure has remarkable curative effects in regulating immune function, anti-tumor, anti-radiation and so on. The exopolysaccharide molecular weight of Schizophyllum is about 40kD. The accumulation of exopolysaccharides from Schizophyllum in the fermentation broth increases the viscosity of the fermentation broth, which is not conducive to the growth of bacteria, so the timely separation of polysaccharides can help increase the yield of exopolysaccharides.

[0067] The 7L fermenter used in the experiment was purchased from Shanghai Gaoji Company, and the inorganic stainless steel membrane module was purchased from Shanghai Kaineng High-tech Engineering Co., Ltd., with a pore size of 0.2 μm. Macropor...

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Abstract

the invention discloses a realizing method of microbe original separating fermenting method through inorganic film in the high-density fermenting domain, which comprises the following parts: ferment tank, inorganic film component, chromatographic column, hollow fiber film component or extracting device, compensating tank, liquid reserving tank and peristaltic pump. the invention can separate object product or side-product from fermenting system, which inhibits microbe seriously.

Description

technical field [0001] The invention is a novel in-situ separation and fermentation technology, which belongs to the field of high-density fermentation of microorganisms; in particular, it relates to a method for realizing in-situ separation and fermentation of microorganisms by using an inorganic membrane. Background technique [0002] The traditional fermentation process is a batch culture process, and the bacteria grow to a certain extent and stop breeding. The growth inhibition in the batch culture process is caused by the following two aspects: (1) substrate inhibition phenomenon: sometimes high concentration of substrate inhibits the cells. The biomass of high-density fermentation can reach 60-150g / l, and a substrate that is 2-5 times the biomass needs to be invested. However, high-concentration nutrients are not conducive to the growth of most microorganisms. According to the Michaelis Kinetic curve, when the nutrient concentration increases to a certain amount, the ...

Claims

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Application Information

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IPC IPC(8): C12P1/00C12N1/00C12M1/00
Inventor 路福平杜连祥李涛史文玉刘逸寒
Owner 天津市新星兽药厂
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