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Composite protein precipitator

A compound protein and precipitant technology, applied in the field of protein precipitant, can solve the problems of large sample loss, chromatographic column damage, cumbersome operation, etc.

Active Publication Date: 2007-02-07
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the low protein precipitation rate of the existing single component protein precipitant, the poor extraction and separation effect, the excessive acidity of 10% trichloroacetic acid and 6% perchloric acid are too strong to the chromatographic column, the cost is high and the The liquid-liquid extraction method is used to extract the defects of long time-consuming, cumbersome operation and large sample loss of drugs in biological samples, thus providing a composite protein precipitant

Method used

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Experimental program
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specific Embodiment approach 1

[0005] Specific embodiment one: this embodiment is that the composite protein precipitant is analytically pure 5-sulfosalicylic acid by 1~1.5 parts by weight ratio, 75~85 parts concentration is analytically pure methanol and 15~25 parts by weight. made with distilled water.

[0006] In this embodiment, 5-sulfosalicylic acid with an analytically pure concentration, methanol with an analytically pure concentration, and distilled water are mixed in parts by weight and dissolved before use.

[0007] In this embodiment, the composite protein precipitant is applied to the pretreatment of biological samples of cardiovascular drugs such as celiprolol hydrochloride, so as to conduct reversed-phase high-performance liquid chromatography analysis. The processing steps of the biological sample (plasma): (1) mix and shake the biological sample and the composite protein precipitation agent of this embodiment according to the volume ratio of 1:1; (2) centrifuge, protein precipitate, and take...

specific Embodiment approach 2

[0011] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the composite protein precipitating agent is analytically pure 5-sulfosalicylic acid, 76~84 parts concentration by weight ratio by 1.1~1.4 parts It is made of analytically pure methanol and 16-24 parts of distilled water. Other steps are the same as those in Embodiment 1.

specific Embodiment approach 3

[0012] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the composite protein precipitant is analytically pure 5-sulfosalicylic acid, 78~82 parts concentration by weight and number ratio by 1.2~1.3 parts concentration It is made of analytically pure methanol and 18-22 parts of distilled water. Other steps are the same as those in Embodiment 1.

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Abstract

the invention discloses a compound protein precipitant, which is characterized by the following: possessing 1-1.5% 5-sulfosalicylic acid, 75-85% carbinol and 15-25% distilled water; making rate of deposition over 99%; damaging little for chromatographic column through pH at weak acid.

Description

technical field [0001] The invention relates to a protein precipitation agent. Background technique [0002] Most of the existing organic solvent protein precipitants, acidic or alkaline protein precipitants and heavy metal protein precipitants are single components, the protein precipitation rate is low, and the extraction and separation effect is poor. Although 10% trichloroacetic acid and 6% perchloric acid have a high protein precipitation rate, and the protein precipitation rate reaches 98.5% to 99.5% in the case of the same volume as the sample, but the acidity is too strong, which will damage the chromatographic column. However, the use of liquid-liquid extraction to extract drugs from biological samples is time-consuming, cumbersome, and the loss of drug components in the sample is large, which greatly affects the efficiency and accuracy of drug analysis in vivo. Meet the needs of providing experimental data quickly and accurately. Contents of the invention [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/30
Inventor 杜智敏张波
Owner HARBIN MEDICAL UNIVERSITY
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