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Pretreatment method for filtering fermentation liquor of bacteria of possessing capsula

A technology of bacterial fermentation and fermentation liquid, which is applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc. It can solve the problems of high viscosity of fermentation liquid, slow phase tangential flow velocity on the membrane surface, and easy blockage of membrane pores , to achieve the effects of simple process route, increased membrane flux, and low equipment cost

Inactive Publication Date: 2006-12-06
TSINGHUA UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The same principle, in membrane filtration, due to the plasticity of the capsule, the pores of the membrane are easily blocked. At the same time, the existence of the capsule makes the fermentation broth have a higher viscosity and the tangential flow velocity on the membrane surface is slower.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0014] (1) fermentation broth: Klebsiella pneumoniae utilizes the fermentation broth of glycerol fermentation to produce 1,3-propanediol, and its components are: 1,3-propanediol 57g / L; 2,3-butanediol 18g / L; Glycerol 31g / L; ethanol 8g / L; lactic acid 23g / L; acetic acid 4g / L; succinic acid 6g / L; OD (650nm) 13.

[0015] (2) Direct filtration effect of fermentation broth:

[0016] Filter paper filtration, the filtration speed decreases to close to 0 soon after the filtration starts, and the filtrate obtained by the filtration is not clear at the same time.

[0017] Add diatomaceous earth suction filter bottle vacuum filtration, the phenomenon is similar to filter paper filtration, can not get a clear filtrate.

[0018] Plate and frame filtration, no clarified filtrate was obtained by adding different types of filter aids.

[0019] Tubular microfiltration membrane filtration, 100 liters of fermentation broth, tubular microfiltration membrane with a membrane area of ​​4 square mete...

example 2

[0028] (1) fermentation broth: Klebsiella pneumoniae utilizes the fermentation broth of glycerol fermentation to produce 1,3-propanediol, and its components are: 1,3-propanediol 58g / L; 2,3-butanediol 17g / L; Glycerin 23g / L; ethanol 7g / L; lactic acid 24g / L; acetic acid 4g / L; succinic acid 6g / L; OD (650nm) 11.

[0029] (2) Direct filtration effect of fermentation broth:

[0030] filter paper, a clear filtrate could not be obtained.

[0031] Add diatomaceous earth and vacuum filter the flask, but no clear filtrate can be obtained.

[0032] Plate and frame filtration, no clarified filtrate was obtained by adding different types of filter aids.

[0033] Hollow fiber microfiltration membrane filtration, 100 liters of fermentation broth, the initial filtration rate is 2 liters / square meter.hour, the end speed is 1 liter / square meter.hour, and the residue is 30 liters.

[0034] (3) Pretreatment conditions:

[0035] Hydrochloric acid for acid adjustment, adjust pH3.0

[0036] Insul...

example 3

[0042] (1) fermentation broth: Klebsiella pneumoniae utilizes the fermentation broth of glycerol fermentation to produce 1,3-propanediol, and its components are: 1,3-propanediol 61g / L; 2,3-butanediol 19g / L; Glycerin 30g / L; ethanol 4g / L; lactic acid 12g / L; acetic acid 2g / L; succinic acid 4g / L; OD (650nm) 9.

[0043] (2) Direct filtration effect of fermentation broth:

[0044] filter paper, a clear filtrate could not be obtained.

[0045] Add perlite suction filter bottle and vacuum filter, can not get a clear filtrate.

[0046] (3) Pretreatment conditions:

[0047] Sodium hydroxide for alkali adjustment, adjust pH13.0

[0048] Insulation, 95 ° C water bath for 0.5 hours.

[0049] (4) Filtering effect after preprocessing:

[0050] Filter with filter paper, the supernatant liquid OD (650nm) value is 0.1.

[0051] Add perlite suction filter bottle for vacuum filtration, add 1.5% perlite filter aid, and the supernatant 650nmOD value is 0.2.

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PUM

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Abstract

This invention provides a method for pre-treating capsular bacteria fermentation liquid with acid or base. The method comprises: (1) adjusting the pH value of the fermentation liquid by an acid to 1-3, or by a base to 11-14; (2) heating to 70-100deg.C, keeping the temperature for 0.3-18 h, and hydrolyzing. The method can hydrolyze and remove the capsules of the capsular bacteria, reduce the plasticity of the bacteria, and decrease the viscosity of the fermentation solution. The method has such advantages as simple process, low cost, low fermentation solution loss and good pretreatment effect.

Description

technical field [0001] The invention belongs to the technical field of bioseparation, and in particular relates to a method for filtering and pretreating fermented liquid of capsulated bacteria. Background technique [0002] Klebsiella pneumoniae, an encapsulated facultative anaerobic bacterium, is a widely studied strain of biological nitrogen fixers. In recent years, the use of Klebsiella pneumoniae to ferment glycerol as a substrate to produce 1,3-propanediol has gradually become a research hotspot. 1,3-propanediol (1,3-Propandiol) is a monomer for the production of polytrimethylene terephthalate (PTT). PTT is a biodegradable polyester material used in advanced textile materials. Compared with polyethylene terephthalate (PET) and polybutylene terephthalate (PDT), the odd number of carbon atoms in 1,3-propanediol gives PTT special properties. The current 1,3-propanediol production methods mainly include the acrolein method and the ethylene oxide method, but these two me...

Claims

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Application Information

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IPC IPC(8): C12P7/06C12R1/22
CPCY02E50/17Y02E50/10
Inventor 刘德华郝健韦明泉乔建援杜风光乔凯
Owner TSINGHUA UNIV
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