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A composite insecticide

A technology of pesticides and solvents, applied in the field of compound pesticides of plant extracts and nuclear polyhedrosis virus, can solve the problems of reduced success rate, increased difficulty and risk, and high cost

Inactive Publication Date: 2006-11-22
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the cost of investing in the research of new chemical pesticides is getting higher and higher, the success rate is declining, and the difficulty and risk are increasing.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0020] 1. Preparation of virus liquid

[0021] The karyopolyhedrons purified from the carcasses of beet armyworms that were naturally infected and killed in the field were diluted to different concentrations, and fed to the 4th instar larvae artificially reared in large quantities in the room. Treat 2 repetitions, 20 larvae per repetition, feed two larvae in each test tube, replace non-toxic feed after 48 hours of poisoning, observe the incidence of larvae every day, collect diseased insects before frequent death, add sterile water after body surface disinfection, place Store at 4°C;

[0022] Weigh a certain amount of dead bodies of diseases and insects and add 2-3 times of sterile water to dilute, grind twice with a mortar, filter with 2 layers of gauze, and take the filtrate for later use; take out the filtrate and dilute it with distilled water at 1:10, and mix the solution with 3200r / Centrifuge for 30 minutes, discard the supernatant, add an appropriate amount of 0.1% ne...

Embodiment 1

[0025] Weigh a certain amount of dried chili powder and place it in a 1000ml wide-mouth bottle, add 90% ethanol solvent, soak at room temperature for 12 hours, take out the soaking liquid, add 90% ethanol solvent to soak for 12 hours, and repeat the extraction for 3 times, then Rinse the residue twice with 90% ethanol solvent, filter with filter paper and combine all the filtrates. The filtrate was concentrated by a rotary evaporator (45-50°C), and finally the mother liquor was prepared by adding 1 gram of dry powder and 1ml of water to constant volume, and the mother liquor was sealed and stored in a refrigerator at 0-4°C until use.

Embodiment 2

[0027] Weigh a certain amount of degeria dry powder and place it in a 1000ml wide-mouth bottle, add 90% acetone solvent, soak for 12 hours at room temperature, take out the soaking liquid, add 90% acetone solvent for soaking for 12 hours, and repeat the extraction for 3 times. Then wash the residue twice with 90% acetone solvent, filter with filter paper and combine all the filtrates. The filtrate was concentrated by a rotary evaporator (45-50°C), and finally the mother liquor was prepared by adding 1 gram of dry powder and 1ml of water to constant volume, and the mother liquor was sealed and stored in a refrigerator at 0-4°C until use.

[0028] 3. Preparation of compound insecticide

[0029] The capsicum extract and the nuclear polyhedrosis virus solution are mixed at normal temperature and pressure at a ratio of 2:8 to obtain a compound insecticide; Mix under normal temperature and normal pressure to obtain a compound insecticide.

[0030] The invention has higher toxicity...

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PUM

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Abstract

The present invention discloses one kind of compound pesticide prepared through mixing plant extract liquid and nuclear polyhedron virus liquid in the volume ratio of 1 / 9-9 at normal temperature. The plant extract liquid may be capsaicim or rotenone. The compound pesticide has high toxicity, high pesticidal effect, obvious synergistic effect, relatively low cost and wide latent market.

Description

technical field [0001] The invention relates to a biological insecticide, in particular to a compound insecticide of plant extract and nuclear polyhedrosis virus, and belongs to the field of biological technology. Background technique [0002] With China's accession to WTO, integration with foreign agriculture and participation in foreign economic competition, it is imminent to engage in export agriculture. The key factor affecting the export of vegetables in my country is that the safety and hygiene of exported vegetables do not meet the requirements of the importing country. Take the vegetables exported to Japan in 2001 as an example. In 2001, nearly 20-30 batches of fresh vegetables exported to Japan were sold by Japan. It was found that the pesticide residues exceeded the standard. Judging from the current varieties of vegetables exported by my country, the export varieties are relatively single, mainly green onions and garlic products. The products are mainly sold to Jap...

Claims

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Application Information

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IPC IPC(8): A01N65/38A01N65/20
CPCY02A50/30
Inventor 蒋杰贤季香云
Owner SHANGHAI ACAD OF AGRI SCI
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