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Method for preparing bacterial cellulose wet-coating for artificial skin

A bacterial cellulose and artificial skin technology, applied in medical science, prosthesis, etc., can solve the problems of high price, narrow application range, virus invasion cost, etc., achieve good toughness and adhesion, simple preservation method, reduce virus Effects of Intrusion Hazards

Inactive Publication Date: 2006-10-25
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, pigskin and allogeneic human skin materials (made of polymers combined with other chemical substances—including substances extracted from shark cartilage) are mostly used in China to treat skin defects. These biological dressings have single functions and narrow application ranges. There are hidden dangers such as being susceptible to bacteria or viruses, and prone to rejection
Although the United States, Japan and other countries have successfully found ways to use raw materials such as collagen or chitin to produce biological dressings, it is currently difficult to apply them on a large scale in China and other parts of the world due to problems such as high prices.
Moreover, the shape and structure of these materials used for artificial skin need to be processed and sterilized in various processes, and some also need to be processed and shaped. The complexity of the manufacturing process is more likely to lead to potential virus invasion and high costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Take 0.2mL of liquid medium with a sterile pipette, drop it into the ampoule tube of Acetobacter xylinum strain, shake gently, so that the freeze-dried bacteria dissolve into a suspension, absorb all the suspension of bacteria, Transplant on the slant medium, culture at 25°C for 1 day, transfer it to the same slant medium, and transfer it every 3 days, and repeat this 6 times to complete the activation process of the strain;

[0021] (2) Get a ring of activated slant strains and insert them into the seed medium, stir and cultivate them for 20h under a magnetic stirrer with a rotating speed of 400rpm / min at 25°C, then insert them into the liquid medium, and change the inoculum size (3% ~9%), other conditions are constant, insert liquid culture medium, observe on the 7th day, the thickness of bacterial cellulose reaches maximum value 2.3mm when the inoculum size is 7%, thereafter occurs the trend of thickness reduction.

[0022] (3) After rapid separation and purifica...

Embodiment 2

[0024] (1) Take 0.6ml of liquid culture medium with a sterile pipette, drop it into the ampoule tube of Gluconobater spp strain, shake gently, so that the freeze-dried bacteria dissolve into a suspension, absorb all the suspension of the bacteria, and transplant Cultivate on the slant medium at 33°C for 3 days, transfer it to the same slant medium, transfer every 1 day, and repeat this 10 times to complete the activation process of the strain;

[0025] (2) Get a ring of activated slant strains and insert them into the seed culture medium, stir and cultivate them for 25h under a magnetic stirrer with a rotating speed of 450rpm / min at 33°C, then insert them into the liquid culture medium, and change the pH value (5.0~ 7.0), other conditions are constant, insert liquid culture medium, observe on the 7th day, the thickness of bacterial cellulose film reaches maximum value 2.2mm when pH value is 5.6, and the trend of thickness reduction occurs thereafter.

[0026] (3) After rapid s...

Embodiment 3

[0028] (1) Take 0.4ml of liquid medium with a sterile pipette, drop it into the ampoule tube of the Rhizobium trifolii strain, shake gently, so that the freeze-dried bacteria dissolve into a suspension, absorb all the suspension of the bacteria, and transplant Cultivate on slant medium at 30°C for 2 days, transfer it to the same slant medium, transfer once every 2 days, and repeat this 8 times to complete the activation process of the strain;

[0029] (2) Get a ring of activated slant strains and insert them into the seed culture medium, stir and cultivate them under a magnetic stirrer at 420rpm / min at 30°C for 22h, then insert them into the liquid culture medium, and change the depth of the liquid surface (4 ~10cm), other conditions are constant, insert liquid culture medium, observe on the 7th day, the thickness of bacterial cellulose film reaches maximum value 2.6mm when liquid surface depth 4cm, thereafter occurs the trend of thickness reduction.

[0030](3) After rapid se...

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Abstract

The present invention discloses a preparation method of bacterial cellulose wet membrane for artificial skin. Said method includes the following steps: (1), transferring liquid culture medium onto slant culture medium to implement activation of strain; (2), inoculating the activated slant strain into seed culture medium, stirring and making culture for 20-25 hr, inoculating said strain into liquid culture medium, making culture for 6-10 days; (3), quickly-separating and purifying cellulose wet membrane, storing in refrigerator.

Description

technical field [0001] The invention relates to a preparation method of bacterial cellulose wet film, in particular to a preparation method of bacterial cellulose wet film for artificial skin Background technique [0002] Cellulose is the main component of natural plant fiber. Bacterial cellulose is a type of cellulose produced by microorganisms. From the molecular composition of cellulose, both are composed of β-D-glucose through β-1,4-glucoside The straight chains formed by bonds are parallel to each other, not in a helical conformation, and have no branched structure, also known as β-1,4-glucose. Bacterial cellulose and plant cellulose are chemically the same, but bacterial cellulose, as a new type of biological material, has many unique properties. (1) High Young's modulus, high tensile strength and excellent shape retention. (2) Ultrafine (nanoscale), the formation process of the bacterial fiber ribbon is as follows: the diameter of the cellulose microfibril secreted ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/14
Inventor 王华平孙晓玉邹瑜陈仕艳颜志勇石帅科方正然王彪张玉梅
Owner DONGHUA UNIV
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