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Maladera sp. spermary chromosome production and observation method

A technology of chromosomes and beetles, applied in the biological field, can solve problems such as inaccurate identification, unspecified species, inaccurate identification, etc.

Inactive Publication Date: 2006-09-06
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The silk beetle Maladera sp. belongs to the order Coleoptera, the velvet beetle family, and the genus Sericoptera. It is the main scarab pest that eats and damages tobacco and other crops. The morphological identification method cannot be accurately identified, so the classification of the silk beetle is still only in the genus, and the species has not yet been determined.
However, the morphological identification method cannot accurately identify scarabs of the same genus that are very similar in shape. For those who do not have the conditions for molecular biology experiments, the molecular biological identification method is also subject to certain restrictions, and there is no way to use karyotype analysis. Successful chromosome production observation method, the chromosome shape and counting of the insect cannot be clearly observed after using the current chromosome production method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The testes of adult beetle beetles were put into 0.05% colchicine solution for 6 hours.

[0014] The concentration of colchicine solution is 0.1%; the preparation of Giemsa solution is 1g of Giemsa reagent, 50ml of glycerol, and 50ml of methanol. First, 1g of Giemsa reagent is put into a mortar, then 5ml of glycerin is added to grind to make it dissolve into a homogenate, and then Add glycerin, mix, put into bottle and shake well, then add methanol.

[0015] Preparation of glass slide specimen: Take out the testis from the colchicine immersion solution, wash it and divide it into testicular tubules under a dissecting microscope, first treat it with hypotonicity in 0.4% KCl solution and distilled water for 30 minutes, and transfer it into the fixative solution (methanol: Glacial acetic acid = 3:1) after 30 minutes, take out the testis tubule, put a testis tubule on the glass slide, drip a drop of 0.05% colchicine solution, cover with a clean cover glass, and knock the te...

Embodiment 2

[0020] Take the testes of adult beetle beetles and put them into 0.05% colchicine solution for 14 hours.

[0021] The concentration of the colchicine solution is 0.3%. The Giemsa solution is prepared by 1 g of Giemsa reagent, 50 ml of glycerol, and 50 ml of methanol. First, 1 g of Giemsa reagent is put into a mortar, and then 5 ml of glycerin is added to grind to make it dissolve into a homogenate. Add glycerin, mix, put into bottle and shake well, then add methanol.

[0022] Preparation of glass slide specimen: Take out the testis from the colchicine immersion solution, wash it and divide it into testicular tubules under a dissecting microscope, first treat it with hypotonicity in 0.4% KCl solution and distilled water for 30 minutes, and transfer it into the fixative solution (methanol: After 60 min of glacial acetic acid = 3:1), take out the testis tubule, put a testis tubule on a glass slide, add a drop of 0.05% colchicine solution, cover with a clean coverslip, and tap to ...

Embodiment 3

[0027] Take the testes of adult silk beetles and put them into 0.05% colchicine solution for 10 hours.

[0028] The concentration of colchicine solution is 0.2%, and the preparation of Giemsa solution is 1g of Giemsa reagent, 50ml of glycerol, and 50ml of methanol. First, 1g of Giemsa reagent is put into a mortar, and then 5ml of glycerin is added to grind to make it dissolve into a homogenate. Add glycerin, mix, put into bottle and shake well, then add methanol.

[0029] Preparation of glass slide specimen: Take out the testis from the colchicine immersion solution, wash it and divide it into testicular tubules under a dissecting microscope, first treat it with hypotonicity in 0.4% KCl solution and distilled water for 30 minutes, and transfer it into the fixative solution (methanol: Glacial acetic acid = 3:1) after 45 minutes, take out the testis tubule, put a testis tubule on a glass slide, add a drop of 0.05% colchicine solution, cover with a clean cover glass, and knock th...

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PUM

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Abstract

Wherein, with adult sperm cell of Maladera sp. As material, pre-processing for 6-14h with colchicine; using 0.4%KCl to hypotonic reaction for 30min; curing with methaol and glacial acetic acid (3:1) for 30-60min; finally, dyeing with Giemsa for 10-20min. this invention obtains clear chromosome with number as n = 9.

Description

Technical field: [0001] The invention belongs to the field of biotechnology and provides a method for making and observing testicular chromosomes of silk beetles. Background technique: [0002] The silk beetle Maladera sp. belongs to the order Coleoptera, the velvet beetle family, and the genus Sericoptera. It is the main scarab pest that eats and damages tobacco and other crops. The morphological identification method can not be accurately identified, so the classification of the silk beetle is still only in the genus, and the species has not yet been determined. However, the morphological identification method cannot accurately identify scarabs of the same genus that are very similar in shape. For those who do not have the conditions for molecular biology experiments, the molecular biological identification method is also subject to certain restrictions, and there is no way to use karyotype analysis. The successful observation method of chromosome production, the chromoso...

Claims

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Application Information

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IPC IPC(8): G01N33/483G01N21/25G01N1/30
Inventor 陈斌李正跃桂富荣孙跃先严乃胜
Owner YUNNAN AGRICULTURAL UNIVERSITY
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