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Chinese caterpillar fungus cultivation method and its special-purpose culture medium

A technology of Cordyceps sinensis and its cultivation method, applied in the direction of fungi, etc., which can solve the problems of inapplicable medium and cultivation conditions, slow growth rate, low production yield, etc., achieve good social and economic benefits, fast growth rate, and low cost Effect

Inactive Publication Date: 2006-07-26
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Cordyceps sinensis is located in a special ecological environment, with an average annual temperature of only about 1.5°C, making its growth temperature low and its growth rate slow
In industrial fermentation, due to low temperature and slow growth, problems of low production yield, large consumption and high cost are often caused
[0005] Many units in China have carried out research on the artificial cultivation of Cordyceps sinensis. Although some have entered the stage of industrial production, studies have confirmed that many fermented products called "Cordyceps sinensis mycelium" have no connection with natural Cordyceps sinensis at the molecular level. The medium and culture conditions cannot be applied to real Cordyceps sinensis strains; and the fermentation production with Cordyceps sinensis strains still has the problems of long fermentation period, low yield and high cost, and its culture medium and culture conditions need to be optimized urgently

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Production of Cordyceps Mycelium

[0021] Cordyceps special medium: milk powder 50.0g, sucrose 20.0g, peptone 5.0g, yeast extract 1.0g, soybean oil 3.0g, potato 200g, dilute to 1000ml with water.

[0022] Control medium: 200 g potatoes, 20.0 g glucose, and dilute to 1000 ml with water.

[0023] 1. Preparation of culture medium

[0024] Potato processing: Peel the potatoes, cut into small pieces and boil for half an hour, then filter with gauze and add water to make up to 1000 ml.

[0025] Take 1000 ml of potato juice, add milk powder, sucrose, peptone, yeast extract, and soybean oil according to the above medium formula, dissolve, and finally dilute to 1000 ml with water.

[0026] 2. Production of Cordyceps Mycelium

[0027] Isolate the strain from natural Cordyceps sinensis, purify it, and carry out molecular biological identification to confirm that it is a true strain of Cordyceps sinensis. At the initial pH value of 6.0, the Cordyceps strains were inoculated ...

Embodiment 2

[0029] Example 2. Production of Cordyceps Mycelium

[0030] Special culture medium for Cordyceps: 50.0 g corn flour, 20.0 g sucrose, 3.0 g yeast extract, 3.0 g soybean oil, 200 g potato, and dilute to 1000 ml with water.

[0031] Control medium: 200 g potatoes, 20.0 g glucose, and dilute to 1000 ml with water.

[0032] Medium preparation: boil corn flour for half an hour, add potatoes and boil for half an hour, then filter with gauze, add sucrose, peptone, yeast extract, soybean oil according to the above formula, and finally dilute to 1000 ml with water.

[0033] Isolate the strain from natural Cordyceps sinensis, purify it, and carry out molecular biological identification to confirm that it is a true strain of Cordyceps sinensis. At the initial pH value of 6.0, the Cordyceps strains were inoculated into the above-mentioned Cordyceps special medium at an inoculum of 15%, and the bottled volume was 1 / 5 of the volume of the flask, and then at 18℃, rotating at 100rpm, 24 Shaking cu...

Embodiment 3

[0035] Example 3. Production of Cordyceps Mycelium

[0036] Cordyceps special medium: 50.0 grams of soybean meal, 20.0 grams of glucose, 5.0 grams of peptone, 3.0 grams of soybean oil, 200 grams of potatoes, and dilute the volume to 1000 ml with water.

[0037] Control medium: 200 g potatoes, 20.0 g glucose, and dilute to 1000 ml with water.

[0038] The medium was prepared in the same way as in Example 2.

[0039]Isolate the strain from natural Cordyceps sinensis, purify it, and carry out molecular biological identification to confirm that it is a true strain of Cordyceps sinensis. At the initial pH value of 6.0, the Cordyceps strains were inoculated into the above-mentioned Cordyceps special medium at an inoculum of 15%, and the bottled volume was 1 / 5 of the volume of the flask, and then at 18℃, rotating at 100rpm, 24 Shaking culture was performed for 25-30 days under dark conditions for 25-30 days to obtain Cordyceps mycelium, and the mycelium yield reached 21.5g / L. At the same...

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Abstract

The invention discloses a Chinese caterpillar fungus cultivation method and its special-purpose culture medium, wherein the formulation comprises composite nitrogen source 10.0-50.0g, potato 200g, monosaccharide or disaccharide 0-20.0g, composite vitamin source 0-5.0g, vegetable oil 0-3.0g, peptone 0-5.0g, and balancing water. The culture process consists of, at a starting pH of 4.8-7.2, inoculating Cordyceps sinensis bacterial into single-purpose Cordyceps sinensis culture medium by the amount of 5-20%, then carrying out oscillation culturing at 12-21 deg C and 100-150rpm under natural daylight or 24h dark condition.

Description

Technical field [0001] The invention relates to a culture method of Cordyceps sinensis and its special culture medium. Background technique [0002] Cordyceps sinensis (Berk.) Sacc. is a member of Ascomycota, Clavicipitaceae, and Cordyceps [Cordyceps (Fr.) Link]. It is parasitic in the Lepidoptera Hepialidae. On the insect larvae, it erodes the insect body, causes it to die and forms a sclerotium (the outer surface is the skin of the insect larva), and the following spring and summer produce a sub-seed to form a medicinal complex. Cordyceps sinensis is a precious Chinese medicinal material in my country's medical treasure house. It is as famous as ginseng and deer antler. Cordyceps sinensis has a long history of medicinal use in my country, and it was officially included in the Pharmacopoeia of the People's Republic of China in 1963. [0003] Modern pharmacological studies have confirmed that Cordyceps sinensis has immunoregulatory function, can eliminate phlegm and relieve asthm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 姚一建董彩虹王波谢雪钦
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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