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Method for preparing complete water soluble low molecular weight chitosan/chitooligosaccharace

A water-soluble chitosan and low-molecular-weight technology, applied in the field of chemical biology of renewable resources, can solve problems such as not easy to obtain low-molecular-weight chitosan/chitooligosaccharide, severe chemical degradation process, cumbersome reaction process, etc., to achieve Improve human immunity, inhibit tumor cell growth, and broaden the effect of application

Inactive Publication Date: 2006-07-19
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This reaction process is cumbersome and not easy to control
Especially the chemical degradation process is severe, it is not easy to obtain low molecular weight chitosan / chitooligosaccharide, and the product structure is modified

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: 75% deacetylation degree, the chitosan of weight-average molecular weight 850,000 is dissolved in 100 milliliters of 10% (v / v) acetic acid solution, and the prepared concentration is the chitosan acetic acid solution of 0.040g / ml. Acetic anhydride with a chitosan molar ratio of 5:3 was dissolved in 80 ml of absolute ethanol. Slowly add the mixed solution into the chitosan solution under magnetic stirring, and continuously stir at room temperature for 5 hours to obtain a water-soluble chitosan solution. The water-soluble chitosan solution is rotary evaporated to remove the reaction solvent absolute ethanol, then the pH is adjusted to 8-9 with 3mol / l sodium hydroxide solution, put into a dialysis bag and dialyzed with water for 24 hours, the dialysate is filtered to remove insoluble matter, and the filtrate is used Concentrate with a rotary evaporator, precipitate with ethanol, wash with ethanol, and dry in vacuum to obtain water-soluble chitosan powder. Wa...

Embodiment 2

[0012] Embodiment 2: 82% of deacetylation degree, the chitosan of weight average molecular weight 600,000 is dissolved in 100 milliliters of 3% (v / v) acetic acid solution, and the prepared concentration is the chitosan acetic acid solution of 0.030g / ml. Acetic anhydride with chitosan molar ratio of 5:4 was dissolved in 60 ml of absolute ethanol. Slowly add the mixed solution into the chitosan solution under magnetic stirring, and continuously stir at room temperature for 3 hours to obtain a water-soluble chitosan solution. The water-soluble chitosan solution is rotary evaporated to remove the reaction solvent absolute ethanol, then the pH is adjusted to 8-9 with 2mol / l sodium hydroxide solution, put into a dialysis bag and dialyzed with water for 48 hours, the dialysate is filtered to remove insoluble matter, and the filtrate is used Concentrate with a rotary evaporator, precipitate with ethanol, wash with ethanol, and dry in vacuum to obtain water-soluble chitosan powder. Wa...

Embodiment 3

[0013] Embodiment 3: the chitosan with deacetylation degree 89%, weight-average molecular weight 440,000 is dissolved in 100 milliliters of 1% (v / v) acetic acid solution, and the prepared concentration is the chitosan acetic acid solution of 0.025g / ml. Under magnetic stirring, acetic anhydride with a molar ratio of chitosan of 5:2 was dissolved in 150 ml of absolute ethanol. Slowly add the mixed solution into the chitosan solution, and stir continuously at room temperature for 1 hour to obtain a water-soluble chitosan solution. The water-soluble chitosan solution is rotary evaporated to remove the reaction solvent absolute ethanol, then the pH is adjusted to 8-9 with 5mol / l sodium hydroxide solution, put into a dialysis bag and dialyzed with water for 36 hours, the dialysate is filtered to remove insoluble matter, and the filtrate is used Concentrate with a rotary evaporator, precipitate with ethanol, wash with ethanol, and dry in vacuum to obtain water-soluble chitosan powder...

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PUM

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Abstract

The disclosed preparation method for chitose / chitosan oligosaccharide with low molecular weight as 0.18-15mega and well water-solubility comprises: preparing water-soluble chitosan by acetylization; degrading the product with non-specific hydrolase while controlling the enzyme dosage and reaction time; adjusting the degraded product pH value to 8-9 with KOH or NaOH solution; depositing with ethanol, cleaning, and vacuum drying the final product with free amidogen that can promote wound healing, restrains tumor cell growth and improves human immunity. Compared with prior art, this invention is convenient to control for wide application.

Description

technical field [0001] The invention relates to a simple preparation method of completely water-soluble low-molecular-weight chitosan / chitooligosaccharide, which belongs to the field of chemical biology of renewable resources. Background technique [0002] The water-soluble chitosan is a product obtained by controlling the degree of deacetylation to 40-60% after the chitosan is acetylated. Water-soluble chitosans of any molecular weight can be dissolved in water at low concentrations, while low-molecular-weight water-soluble chitosans and chitosan oligosaccharides can also be dissolved in water at higher concentrations, and have a higher molecular weight than large-molecular-weight water-soluble chitosans. Sugar for better biocompatibility. Water-soluble chitosan with different molecular weight has different biological and physiological activities, so the preparation of water-soluble chitosan with different molecular weight is of great significance for its application in fo...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 杜予民李瑾
Owner WUHAN UNIV
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