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Facultative anaerobe of degrading polychlorinated diphenyl and obtainding process

A technology of facultative anaerobic bacteria and polychlorinated biphenyls, applied in the field of environmental bioengineering, can solve the problem that PCBs cannot be effectively biodegraded

Inactive Publication Date: 2006-06-28
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The basic concept of the present invention is: firstly, a large number of bacterial strains capable of degrading PCBs are screened, separated, and purified from PCBs pollution points, and high-efficiency bacterial strains are further screened out to solve the problem that the ortho or 2,3 or 3,4 sites are replaced by chlorine and the problem that highly toxic PCBs substituted with more than 6 chlorine cannot be effectively biodegraded

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Screening of PCBs Degrading Strains

[0049] The Raoultella terrigena LY402 degrading strain was obtained through steps such as screening, isolation and purification, and enhanced domestication, as follows:

[0050] The first step, preliminary screening

[0051] Prepare a liquid synthetic medium that uses biphenyl as a carbon source and contains various minerals. Add PCBs-contaminated soil to the synthetic medium. Bacteria that can grow using biphenyl will multiply in large numbers, and they will be subcultured repeatedly for 5~ 10 times, according to the ability of the subcultured mixed bacteria to degrade 2,3′,4,4′-tetrachlorobiphenyl to determine the object for further strain isolation and purification.

[0052] Configure liquid synthetic medium with biphenyl content of 1 and 3g / L respectively. The composition of mineral salts in the medium is the same, and the contents are: KH 2 PO 4 1.7g / L, K 2 HPO 4 4.4g / L, NH 4 Cl2.1g / L, NaCl3.0g / L, yeast extract 0.05g / L, M...

Embodiment 2

[0060] Identification and Characterization of Strains:

[0061] After identification by the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures, the species of the No. 3 strain is Raoultella terrigena, another name is Klebsiella terrigena, and it is named LY402. CGMCC), the address is No. 13, North Zhongguancun, Haidian District, Beijing, the preservation date is July 20, 2005, and the preservation center number is 1420. This bacterial strain is a facultative anaerobic bacterium. The temperature and acidity ranges in which the strain can grow were tested. First, utilize 200 milliliters of sucrose lipid synthesis medium to carry out suspension culture to Raoultella terrigena LY402, the culture condition and the composition of medium are the same as the first step in the embodiment 1, and the bacterium density reaches OD with ultraviolet spectrophotometer detection 650nm When =1.0, stop cultivating, the bacterium suspension is eve...

Embodiment 3

[0063] Screening of carbon sources to replace biphenyl and promote PCBs biodegradation

[0064] Using biphenyl, glucose, glycerin, nonionic surfactants OP-10, Tween-20, Tween-80, Triton-100 and biosurfactant sucrose lipid as carbon sources respectively, plus the initial step in the first step of Example 1 Sieve the minerals with the same content in the culture medium to prepare 8 kinds of liquid synthetic culture medium, wherein the amount of carbon source is 2g / L. Take 1 ml of Raoultella terrigena LY402 bacterial suspension, add it to the above 50 ml of the 8 kinds of medium containing different carbon sources, and culture it in a shaker at 30°C and 150 rpm for 3-5 days to make it reach the bacterial density OD 650nm >2.0, take it out and subculture for 3 times. Centrifuge 50 ml of bacterial suspension cultured with different carbon sources, take the precipitated bacterial sludge, and add it to 0.1mol / L phosphate buffer to make it OD 650nm = 1.0, add 2,3', 4,4'-tetrachloro...

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PUM

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Abstract

The invention relates to degrading polychlorinated biphenyl facultative anaerobe and gaining method in environment bioengineering field. It includes the following steps: sampling; screening; separating; purifying; screening and domesticating PCBs degrading strain. The new strain raoultella terrigena LY402 is grained. The strain is facultative anaerobe, and utilizes sucrose fat as carbon source to degrade 2,3,4,4-quadri-chlordiphenyl. The degrading rate of the aroclor1242 is 98.02-99.15% in 70h under oxygen; but aroclor1260 is 96.55% in 8 days under the opposite condition. Its advantages are that it can degrade different sits PCBs; and it can utilize sucrose fat to high effectively degrade PCBs and has no secondary pollution.

Description

technical field [0001] The present invention particularly relates to the screening, separation, purification and domestication of a new bacterial strain Raoultella terrigenaLY402 that efficiently degrades PCBs from the pollution points of polychlorinated biphenyls (PCBs), and the research on the performance of the bacterial strain, which belongs to the field of environmental bioengineering . Background technique [0002] In recent decades, the rapid development of the chemical industry has caused a large amount of hazardous waste to be discharged into the natural environment. Among them, 12 types of organic pollutants have been listed by the United Nations Environment Program because of their strong stability, difficulty in natural biodegradation, and great harm to humans and the environment. The persistent toxic organic pollutants listed as priority by the Department of Control. Polychlorinated biphenyls (PCBs) are one of the most representative types, also known as dioxin...

Claims

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Application Information

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IPC IPC(8): C12N1/00B09C1/10
Inventor 贾凌云文成玉蒋彩平杨凤林
Owner DALIAN UNIV OF TECH
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