Intestine nutrition peptide for piglings
An intestinal and nutritional technology, applied in the field of piglet intestinal nutritional peptides, can solve problems such as inability to solve intestinal mucosal damage, achieve significant economic and social benefits, solve weaning stress, and improve the effect of amylase activity
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Embodiment 1
[0028] The steps of the oligopeptide preparation method are as follows:
[0029] (1) Sterilization
[0030] Soy protein and rapeseed protein are made up of 5:1 enzymatic hydrolysis raw materials, add water to make the liquid-solid ratio 5:1, pasteurize or maintain at 80°C for 10 minutes to sterilize. Soy protein is a mixture of any one or more of protein concentrate, defatted soybean meal, soybean meal, and soybean cake in any ratio; rapeseed protein is a mixture of one or two of rapeseed meal and rapeseed cake in any ratio .
[0031] (2) Enzymatic hydrolysis
[0032] Cool the material and keep it at 35°C, adjust the pH to 6.5, add the Bacillus subtilis neutral protease according to 1.0% (weight ratio) of the soybean protein content, and carry out enzymatic hydrolysis for 2 hours (enzymatic hydrolysis). Activity definition: Under the conditions of pH 6.5 and temperature of 35℃, the enzyme required for the reaction of 5% trichloroacetic acid solubles released by hydrolyzing standar...
Embodiment 2
[0040] The steps of oligopeptide preparation method are as follows:
[0041] (1) Sterilization
[0042] Soy protein and rapeseed protein are composed of 5:2 enzymatic hydrolysis raw materials, add water to make the liquid-solid ratio 10:1, pasteurize or maintain at 85°C for 30 minutes to sterilize. Soy protein is a mixture of any one or more of protein concentrate, defatted soybean meal, soybean meal, and soybean cake in any ratio; rapeseed protein is a mixture of one or two of rapeseed meal and rapeseed cake in any ratio .
[0043] (2) Enzymatic hydrolysis
[0044]Cool the material and keep it at 40°C, adjust the pH to 7.0, add the Bacillus subtilis neutral protease according to 1.5% of the soybean protein content (weight ratio), and carry out enzymatic hydrolysis for 3 hours (enzyme Activity definition: Under the conditions of pH 7.0 and temperature of 40℃, the enzyme required for the reaction of 5% trichloroacetic acid solubles released by hydrolyzing standard casein within 1 M...
Embodiment 3
[0052] The steps of oligopeptide preparation method are as follows:
[0053] (1) Sterilization
[0054] Soy protein and rapeseed protein are composed of enzymatic hydrolysis materials at 5:1.5, add water to make the liquid-solid ratio 7.5:1, pasteurize or maintain at 82.5°C for 20 minutes for sterilization. Soy protein is a mixture of any one or more of protein concentrate, defatted soybean meal, soybean meal, and soybean cake in any ratio; rapeseed protein is a mixture of one or two of rapeseed meal and rapeseed cake in any ratio .
[0055] (2) Enzymatic hydrolysis
[0056] Cool the material and keep it at 37.5°C, adjust the pH to 6.75, add the Bacillus subtilis neutral protease according to 1.25% (weight ratio) of the soybean protein content, and carry out enzymatic hydrolysis for 2.5 hours (enzyme) Activity definition: Under the conditions of pH 6.75 and temperature 37.5℃, the enzyme required for the reaction of 5% trichloroacetic acid solubles released by hydrolyzing standard ...
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