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In situ biological restoring method of petroleum polluted soil

A technology of oil pollution and bioremediation, applied in the restoration of contaminated soil, etc., can solve the problems of prolonged cycle, reduced repair efficiency, poor growth and metabolism of microbial preparations, etc., to achieve good extensibility, improve degradation ability, and improve bioavailability The effect of utilization

Active Publication Date: 2006-06-14
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In-situ remediation occurs in an open soil ecosystem. The heterogeneity of soil structure, the complexity of the ecosystem, and the uncontrollability of environmental factors such as climate often lead to poor growth and metabolism of the added microbial agents, and the degradation activity is affected by indigenous microorganisms. Inhibition, resulting in reduced repair efficiency and prolonged cycle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] In this example, a liquid mixed microbial preparation was prepared by using 1 strain of Yinhan bacteria thorns and 1 strain of Pseudomonas. The preparation method is as follows:

[0016] A 300ml Erlenmeyer flask with a volume of 100ml liquid potato medium was inoculated with C. chinensis, cultured at 28°C and 160rpm for 3 days, and the culture was collected. The fungal content was 20mg dry weight / ml. Pseudomonas was inoculated in a 300ml Erlenmeyer flask with a volume of 100ml LB liquid medium, cultivated for 1 day at 30°C and 160rpm, and the culture was collected. The bacterial content was 10 8 pieces / ml. The obtained fungal culture and bacterial culture were mixed according to a mixing ratio of 10:1 to obtain the liquid mixed microbial preparation used in the examples.

[0017] The additive used in this embodiment is straw, the organic fertilizer is chicken manure, and the inorganic nutrients are urea, diammonium phosphate and potassium sulfate.

[0018] The pollute...

Embodiment 2

[0023] In this example, 1 strain of Phanerochaete chrysosporium and 1 strain of Rhodococcus were used to prepare liquid mixed microbial preparations. The preparation method is as follows:

[0024] Phanerochaete chrysosporium was inoculated in a 300ml Erlenmeyer flask with a volume of 100ml liquid potato medium, cultivated at 30°C and 170rpm for 3 days, and the culture was collected. The fungal content was 60mg dry weight / ml. Pseudomonas was inoculated in a 300ml Erlenmeyer flask with a volume of 100ml LB liquid medium, cultivated at 30°C and 200rpm for 2 days, and the culture was collected, and the bacterial content was 10 12 pieces / ml. The obtained fungal culture and bacterial culture were mixed according to the mixing ratio of 1:10 to obtain the liquid mixed microbial preparation used in the examples.

[0025] The additive used in this embodiment is rice straw, the organic fertilizer is chicken manure, and the inorganic nutrients are urea, diammonium phosphate and potassium...

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Abstract

An in-situ biologic repairing method for the soil polluted by petroleum features that after the fungus-bacteria mixed liquid, soil improver, organic fertilizer and inorganic nutrients are applied to the polluted soil, the soil is irrigated, ploughed, and covered by plastic film.

Description

technical field [0001] The invention relates to a method for enhancing in-situ bioremediation efficiency of mixed microbial preparations on oil-contaminated soil by using additives and various engineering means. It belongs to the in-situ bioremediation technology of oil-contaminated soil. Background technique [0002] The bioremediation technology of oil-contaminated soil can be divided into in-situ bioremediation and ex-situ bioremediation. The in-situ bioremediation method is suitable for the treatment of large-scale pollution, and has the advantages of no need to move the soil, low cost, and simple operation. In the process of in situ bioremediation, it is usually necessary to add microbial agents with high-efficiency degradation of petroleum hydrocarbons. In-situ remediation occurs in an open soil ecosystem. The heterogeneity of soil structure, the complexity of the ecosystem, and the uncontrollability of environmental factors such as climate often lead to poor growth ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 刘铮韩慧龙王福远佘鹏陈镇邢新会张敏莲金文标张坤江皓
Owner CHINA PETROLEUM & CHEM CORP
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