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Senso-like efficient liquid-phase chromatograph finger print atlas identifying method

A high-performance liquid chromatography and fingerprint technology, which is applied in the field of traditional Chinese medicine fingerprints, can solve the problems of lack of specificity and advancement, the inability to effectively identify the authenticity of toad venom, and the absence of scientific, effective and advanced identification methods for toad venom

Inactive Publication Date: 2006-05-24
SHENYANG PHARMA UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0002] The test-tube reaction of the traditional Chinese medicine toad venom "Chinese Pharmacopoeia" to detect bufotryptamines and bufoligands under the identification item, this method cannot effectively identify the authenticity of toad venom; Cinobufaction-based reference substances are identified, this method lacks specificity and advancement
There is no scientific, effective and advanced identification method for toad venom

Method used

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  • Senso-like efficient liquid-phase chromatograph finger print atlas identifying method
  • Senso-like efficient liquid-phase chromatograph finger print atlas identifying method
  • Senso-like efficient liquid-phase chromatograph finger print atlas identifying method

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Embodiment Construction

[0015] Utilize high-performance liquid chromatography, UV detector, chromatographic workstation, take toad venom sample 25mg, put in the Erlenmeyer flask with stopper, accurately add 20ml of methanol, weigh, heat and reflux for 1hr, let cool, weigh again, Use methanol to make up the lost weight, shake well, and pass through a 0.2 μm filter membrane to obtain the test solution of toad venom. Put the solution of the test product to be tested on the analytical column Phenyl-Hexyl (4.6×250mm, 5μm), mobile phase: methanol (A)-buffer (B, a certain proportion of sodium heptanesulfonate or sodium octanesulfonate , citric acid, potassium dihydrogen phosphate or sodium dihydrogen phosphate solution), gradient elution according to the conditions in the table below, the flow rate is 1.0ml / min, the detection wavelength is 296nm, the injection volume is 20μl, and the retention time is 90min. Unique chemical fingerprints (including characteristic peaks of bufotryptamine and bufagenin), and c...

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Abstract

Solution of sample to be tested in arenobufagin class is obtained through following steps: using high performance liquid chromatography, UV detector, chromatographic work station, the method places arenobufagin sample 25mg to be tested into conical flask with plug; adding methyl alcohol 20ml, weighing up fixed weight; heating up reflow for 1h, cooling and weighing up fixed weight again, using methyl alcohol to supplement weight reduced; shaking to uniformity and passing 0.2 micro filter membrane. Chemical finger print of specific bufotenine class and bufogenin class in arenobufagin class is obtained from following steps: under condition of mobile phase, buffer solution of methyl alcohol, gradient elution is carried out at analytical column; sample size as 20 micro liter, retention time 90min. chemical attribution is carried out for all or most of characteristic peak. The method prepares fingerprint of arenobufagin correctly so as to provide reliable reference for identification.

Description

Technical field: [0001] The invention relates to traditional Chinese medicine fingerprint technology, specifically, it is a high-performance liquid chromatography fingerprint identification method for toad venom. Background technique: [0002] The test-tube reaction of the traditional Chinese medicine toad venom "Chinese Pharmacopoeia" to detect bufotryptamines and bufoligands under the identification item, this method cannot effectively identify the authenticity of toad venom; The identification of Cinobufaction-based reference substances lacks specificity and advancement. There is no scientific, effective and advanced identification method for toad venom. However, scientific, effective and advanced identification methods are extremely important for the application of toad venom. Invention content: [0003] The object of the present invention is to provide a method for identifying toad venom by high-performance liquid chromatography fingerprint, and the high-performance...

Claims

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Application Information

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IPC IPC(8): G01N30/38G01N30/02
Inventor 崔征张屏王东
Owner SHENYANG PHARMA UNIVERSITY
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