Capillary electrophoretic chip with extraining sampling and its using method

Abstract

The entraining sampling capillary electrophoresis chip has auxiliary entraining tunnels on two sides of the sampling tunnel and the separating tunnel to form parallel entraining electric field to inhibit the dilatation of sample molecules in the intersection between the sampling tunnel and the separating tunnel and to raise the separating efficiency and detection sensitivity of the electrophoresis chip. In the same time, the chip can alter the width of sampling band via the entraining electric field strength relative to the sampling electric field, so as to regulate the sampling for fitting different analysis. The present invention may be used in the fast and sensitive separation and detection in inorganic molecule, organic molecule, macro biomolecule, virus, cell, etc.

Description

technical field [0001] The invention relates to a capillary electrophoresis chip in a clamping flow sampling mode and a use method thereof, and the chip can be applied to the fields of chemical and biological analysis and detection. Background technique [0002] The capillary electrophoresis chip technology developed rapidly after the 1990s is based on micro-machining technology, with silicon, glass, plastic (mainly plexiglass), ceramics and silicone rubber as the base material, and with the help of capillary electrophoresis technology, the sample is injected , reaction, separation, detection and other processes are integrated into a multi-functional, efficient and fast micro-laboratory technology. Because capillary electrophoresis chip has the characteristics of small size, high separation efficiency, few samples for analysis, fast analysis speed, and automatic analysis process, it has become the frontier and hot spot of international research. So far, capillary electropho...

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Problems solved by technology

The "double T-shaped" structure has high sensitivity due to the large amount of sample injection, but the separation efficiency is poor due to the wide sample band itself and molecular diffusion

Due to the effect of molecular diffusion and the lateral expansion of the electric field at the intersection of the "cross-shaped" structure, when the sample flows through the intersection in the injection channel during the injection phase, the sample expands to the separation channel, thus greatly It affects the separation efficiency; in order to overcome the above problems, some people apply voltage on the electrodes at both ends of the separation channel during sample injection to implement "shrink" sample injection [Jacobson, S.C.; Hergenrsder, R.: Koutny, L.B.; Warmack, R.J.; and Ramsey, J.M., "Effects of Column Geometry on the Performance of Microchip Electrophoresis Devices", Anal.Chem.66, 1107 (1994).], but this sampling method requires a long injection time, which affects the analysis speed, and the injection strip The belt is irregularly tapered, and if the detection window deviates slightly from the centerline of the separation channel, different detection results will be obtained; in addition, some people use the "narrow sample channel" mode [ZhangCX; Manz A., "Narrow sample channel injectors for capillary electrophoresis onmicrochips ″, Anal.Chem.73, 2656(2001).], but this method has sacrificed the detection sensitivity, so it also has great limitations

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