Use of saccharomyces cerevisiae ERG4 mutants for expressing mammalian glucose transporters
A technology for glucose transport and Saccharomyces cerevisiae, applied in the field of yeast strains, can solve the problems of low transporter activity, inactive transporter, retention, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment Construction
[0091] Use of Yeast Strains
[0092] All yeast strains described here were derived from strain CEN-PK2-1C (MATa leu2-3, 112 ura3-52 trp1-289 his3-Δ1MAL2-8 C SUC2). The preparation of yeast strains with a deletion of the hexose transporter (HXT) gene has been described by Wieczorke et al. -52trp1-289 his3-Δ1 MAL2-8 C SUC2), EBY.VW4000 (MATa Δhxt1-17 Δgal2Δagt1 Δmph2 Δmph3 Δstl1 leu2-3, 112 ura3-52 trp1-289 his3-Δ1 MAL2-8 C SUC2)). The medium is based on 1% yeast extract and 2% peptone (YP), while the minimal medium consists of 0.67% Difco Yeast Nitrogen Base (YNB) without amino acids, and contains additives required for co-nutrients and different carbon source. Yeast cells were grown on a rotary shaker or agar plates at 30°C under aerobic conditions. By measuring the optical density at 600nm (OD 600 ) or measure the diameter of yeast colonies to monitor cell growth.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com