Production method of nutritious fungus feed addictive
A feed additive and production method technology, applied in animal feed, animal feed, bacteria, etc., can solve the problems of different strains, different production processes, different medium formulations, etc., to achieve easy control, no three-waste problem, and improved survival rate Effect
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[0038] The production method of the novel nutritional fungal feed additive comprises the following production steps:
[0039] A. The acquisition of strains
[0040] Paecilomyces cicadae (Mip.) Samson is a widely distributed species.
[0041] B. Bacteria culture in inclined test tube
[0042] Inoculate the isolated and purified strains into inclined test tubes, put them in a 25°C incubator, and cultivate for 7 days, until the hyphae are full of the test tubes and produce spores;
[0043] The weight ratio of the raw materials for the culture medium of the slant test tube is: glucose 40g, peptone 10g, yeast extract powder 10g, agar 20g, add water to 1L, pH6.5.
[0044] C. Liquid shake flask seed culture
[0045] Fill 200ml culture medium in 500ml Erlenmeyer flask, use 1.2Kg / cm 2 Autoclave for 30 minutes, and when it cools to room temperature, inoculate the strains passed through one inclined test tube into a 500ml Erlenmeyer flask culture medium, and place it in a constant te...
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