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Method for preparing radioactive nuclide magnetic microsphere used in vivo

A technology of radionuclide and magnetic microspheres, applied in the field of magnetic materials, can solve the problems of large tissue damage, technical difficulty, large radiation dose, etc., and achieve the effect of solving the rejection reaction

Inactive Publication Date: 2005-03-16
北京倍爱康生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, internal radiotherapy has not yet found an ideal path, and problems such as the accurate positioning and administration of target tissues and the diffusion of radionuclides have been plagued by clinical applications.
3) External irradiation therapy: widely used, but the facilities are expensive and technically difficult
The radiation dose is large, and normal tissue damage is large

Method used

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  • Method for preparing radioactive nuclide magnetic microsphere used in vivo

Examples

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example 1

[0015] Weigh 2700g of ferric chloride, 0.5g of ferric chloride, and 0.1g of aluminum nitrate to make a solution, store it under nitrogen gas, add ammonia water dropwise, stir at room temperature at a speed of 2000rpm, add 10ml of ammonia water dropwise, and heat up to 75°C , stirred at the same speed, reacted for 90 minutes, then added oleic acid to disperse it, and washed with purified water to obtain magnetic ferric oxide particles.

example 2

[0017] Weigh 2g of ferric oxide particles, add 20ml of styrene, 6ml of divinylbenzene, 0.3g of benzoic acid peroxide, add each component separately, ultrasonically disperse for 90 minutes, adjust the pH to 11.0, incubate at 67°C, mix , add 1ml of methacrylic acid, 5ml of potassium persulfate solution, and dilute to 1001 with pure flower water. Add 0.18 g of sodium dodecylbenzenesulfonate and 0.5 g of emulsifier toluene cellulose ether into 100 ml of aqueous solution, and stir at 500 rpm. Magnetic microspheres were prepared after 8 hours.

example 3

[0019] Preparation of nuclide microspheres: prepared according to the instructions (3) 131 I-labeled human serum protein 15mg, weighed 1g of magnetic microspheres, obtained 200mg of magnetic microspheres of about 4μm by centrifugation, washed three times with purified water, added 4ml of purified water, 400μl of 5mg / ml 131 I-human serum albumin, 2g of human serum albumin, stirred at room temperature for 20 hours. Wash with 0.1mmol / l hydrochloric acid three times, add 4ml of 0.1mmol / l hydrochloric acid, mix well, add 20mg of ethylene glycol glyoxal dimer, leave it at room temperature for 60 minutes, add 10μl of 2N sodium hydroxide, and continue mixing Mix for 16 hours. After adding 2000 mg of L-glycine, it was mixed at 37° C. for 2 hours. Wash three times with 0.1mmol / l hydrochloric acid, then wash with 0.9% sodium chloride until there is no radioactivity in the solution, redissolve with 4ml 0.9% sodium chloride, and store at 4°C.

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Abstract

The invention provides a method for preparing radioactive nuclide magnetic microsphere used in vivo for the treatment of vessel embolism and tumor, wherein the process by the invention comprises the steps of, preparing magnetic ferrosic oxide particles, preparing magnetic microballoons, marking protein #+[131] and preparing radioactive nuclide. The invention solves the problems of rejection reaction of heterology antibody and damage of normal tissues.

Description

technical field [0001] The invention belongs to the technical field of magnetic materials, and in particular provides a preparation method of radionuclide magnetic microspheres used in vivo, which are used for tumor blood vessel embolization and tumor treatment. Background technique [0002] Magnetic microspheres are composed of iron ferric oxide or ferric oxide of a single configuration, covered with polymer materials, and coupled with various biological macromolecules on the outside of the polymer, such as monoclonal antibodies, which are immune Microspheres. The use of magnetic microspheres in biological separation began in the 1970s, and is currently widely used in cell separation, protein separation and purification, nucleic acid separation, antibiotics, and microbial separation. In recent years, many researchers have devoted themselves to using magnetic microspheres as new carriers for targeted drug delivery, especially in tumor drugs, which have broad application pro...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K51/02A61P35/00C01G49/08
Inventor 刘振世幺崇正张玉庆
Owner 北京倍爱康生物技术有限公司
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