Medicinal composition, its preparation method and its use

A composition and drug technology, applied in the direction of drug combination, drug delivery, pharmaceutical formulation, etc., can solve the problems not involving Vinaorutong, etc., and achieve the effect of improving brain metabolism

Active Publication Date: 2005-03-09
JILIN SIHUAN PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] None of the above patents involves the combination of Vinaorutong and animal brain extracts to prepare new drugs

Method used

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  • Medicinal composition, its preparation method and its use
  • Medicinal composition, its preparation method and its use

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Take 10kg of fresh healthy pig brain, remove impurities such as fascia, wash with water for injection, add 10L water for injection, homogenate, heat at 80°C, keep for 15 minutes, cool to 42°C, adjust the pH value to 7.5, add 45g trypsin (U.S. DIFCO company, specification: 500g) was enzymatically hydrolyzed at 42°C for 3 hours, the pH value was controlled at 8.5 during the enzymolysis process, taken out, heated to 80°C, and kept for 20 minutes to obtain an enzymatic hydrolyzate;

[0047] After 24 hours at 4°C, centrifuge at 4,000 rpm for 15 minutes, filter the pulp, adjust the pH of the supernatant to 3.5, heat at 95°C, keep for 20 minutes, and centrifuge at 4°C for 12 hours. The condition is 4000 rpm, the centrifugation time is 20 minutes, the pulp is filtered, the filtrate is adjusted to pH 6.5, heated to 90°C, kept for 15 minutes, and then centrifuged at 8°C for 8 hours, the centrifugation condition is 4000 rpm, and the centrifugation time is In 20 minutes, the filtra...

Embodiment 2

[0050] Take 10kg of fresh healthy pig brain, remove impurities such as fascia, wash with water for injection, add 10L water for injection, homogenate, adjust the pH value to 8.2, heat at 80°C, keep for 15 minutes, cool to 42°C, add 80g trypsin (U.S. DIFCO company, specification: 500g) was enzymatically hydrolyzed at 42°C for 10 hours, the pH value was controlled to 8.2 during the enzymolysis process, taken out, heated to 80°C, and kept for 20 minutes to obtain an enzymatic hydrolyzate;

[0051] Lower the temperature of the primary enzymatic hydrolysis solution to 44°C, adjust the pH value to 2.5, add 50 g of pepsin (Xiamen Xinglongda Chemical Reagent Co., Ltd; 1:10000; specification: 100 g), and enzymatic hydrolysis for 10 hours, the process pH value is controlled at 2.5 , and then heated to 90°C for 20 minutes to obtain a secondary enzymolysis solution;

[0052] Centrifuge at a speed of 4000 rpm for 30 minutes, filter the supernatant from a clarification plate, adjust the pH ...

Embodiment 3

[0055] Take 10kg of fresh healthy bovine brain, remove fascia and other impurities, wash with water for injection, add 25L water for injection, homogenate, adjust the pH value to 9.0, heat at 95°C, keep for 30 minutes, cool to 38°C, add 150g of trypsin, keep warm 4 hours, control the pH value to 8.0 during the enzymolysis process, then heat to 85°C and keep for 30 minutes to obtain an enzymolysis solution;

[0056] Place at 8°C for 8 hours, centrifuge at 4000 rpm, centrifuge for 20 minutes, filter the supernatant pulp, adjust the pH of the filtrate to 4.0, heat at 80°C, keep for 30 minutes, place at 5°C for 12 hours, centrifuge, The centrifugation condition is 4000 rpm, the centrifugation time is 30 minutes, the supernatant is filtered, the filtrate is adjusted to pH 7.0, heated to 95°C, kept for 30 minutes, and centrifuged at 7°C for 18 hours, the centrifugation condition is 4000 rpm, The centrifugation time is 30 minutes, and the supernatant is filtered to obtain a filtrate;...

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Abstract

The invention discloses a medicinal composition containing weinaolutong (7, 3', 4'-troxerutin) obtained by hydroxyethylating eldrin and brain extract which is extracted form brain tissue of mammalian except human. The medicinal composition contains 1 to 1000 parts of weinaolutong, by weight, and the weight of the weinaolutong in the medicinal composition is 0.1 to 200 times bigger than total nitrogen content in the brain extract. The invention also discloses preparation method and use of the medicinal composition. The medicinal composition can restrain platelet agglutination thereby preventing thrombosis, and can regulate and improve brain supersession, and is used for treating acute and chronic cerebrovascular disease and sequela such as brain dysfunctional caused by craniocerebral trauma and cerebrovascular disease; and can also be used for treating occlusive syndrome, arteriosclerosis, thrombosis phlebitis, capillary bleeding and dropsy caused by elevation of vascular permeability.

Description

technical field [0001] The present invention relates to a pharmaceutical composition, its preparation method and its application, in particular to a pharmaceutical composition of brain extract extracted from the brain tissue of mammals other than humans and venaolutong, and its preparation The method and its use in the preparation of medicines for treating acute and chronic cerebrovascular diseases and the sequelae caused by them, such as brain dysfunction. Background technique [0002] Vinaolutong (also known as troxerutin) is a water-soluble flavonoid compound obtained by semi-synthesis of rutin through hydroxyethylation. D)-based mixture of hydroxyethyl rutin, generally used for medicine, calculated as anhydrous substance, containing troxerutin (C 33 h 42 o 19 ) shall not be less than 80.0% (for injection) and 60.0% (for oral use). Vinaorutong can inhibit the aggregation of platelets and has the effect of preventing thrombosis. At the same time, it can resist vascula...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K35/30A61P7/02A61P7/04A61P9/00A61P9/10
Inventor 张志
Owner JILIN SIHUAN PHARM CO LTD
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