Bacillus D747 strain, plant disease controlling agents or insect pest controlling agents using the same and control method using the agents

A technology for bacillus and plant diseases, which is applied in the field of pest control agents and plant disease control agents, and can solve the problems of not seeing bacillus bacteria, etc.

Inactive Publication Date: 2005-03-02
KUMIAI CHEM IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, there are many research examples on the use of antagonistic microorganisms. In addition, although there are many researches on the use of Bacillus for disease control among antagonistic microorganisms, no Bacillus for broad-spectrum disease control has been seen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Embodiment 1: the cultivation of D747 bacterial strain

[0085] D747 strain was isolated from the air of Kikugawa Street, Ogasasa-gun, Shizuoka Prefecture. Specifically, in order to isolate microorganisms floating in the air in Kamo, Kikugawa-dori, Ogasa-gun, Shizuoka Prefecture, the plate culture medium added with the potato-dextrose agar medium was removed from the cover and left in contact with the air for 10 minutes. This was cultured at 27° C. for 3 days, and the formed colonies were isolated. The isolated colonies were shaken and cultured in potato-glucose liquid medium, and strains that acted on plant pathogenic bacteria were found, and strain D747 was isolated.

[0086] The isolated D747 strain contained glucose 1%, soluble starch 2%, polyptone 0.5%, dry yeast 1%, defatted soybean 1%, KH 2 PO 4 0.2%, NaCl 0.2%, calcium carbonate 0.3%, pH 6.0 culture medium, 27 ° C, 120 rpm shaking culture for 3 days, centrifuged to collect bacteria (10,000 × g, 15 minutes), s...

Embodiment 2

[0087] Embodiment 2: the control effect test of rice blast

[0088] Ten rice seeds (variety: Aichi Asahi) were sown in a biscuit pot each having a diameter of 7.5 cm, and cultured in a greenhouse. Sprinkle 10 ml / pot of the D747 suspension obtained in Example 1 above into the rice with the fourth leaf spread, and after air-drying, inoculate the spore suspension of Pyricularia oryzae by spraying and manage in a wet room. Five days after the inoculation, the number of lesions in the fourth leaf was checked, and the degree of disease was evaluated according to the criteria in Table 1 below. The experimental results are shown in Table 2.

[0089] A

Embodiment 3

[0090] Example 3 Control Effect Experiment of Rice Sheath Blight

[0091]10 grains of rice seeds (variety: Jinnanfeng) were sown in each biscuit pot with a diameter of 6.0 cm, and cultured in a greenhouse. 10 ml / pot of the suspension of the D747 strain obtained in Example 1 above was sprinkled on rice at the second to third leaf stage, air-dried, inoculated with rice sheath blight fungus (Thanatephor uscucumeris), and managed in a greenhouse. Five days after the inoculation, the number of lesion spots was checked, and the degree of disease was evaluated according to the criteria in Table 1 below. The experimental results are shown in Table 2.

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PUM

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Abstract

The present invention relates to a novel strain of Bacillus sp. D747 (FERM BP-8234). In addition, the present invention also relates to an agent for controlling plant diseases and an agent for controlling insect pests, comprising the Bacillus sp. D747 strain, and relates to a control method using the agents described above. By administering cultures of Bacillus sp. D747 (including the viable bacteria) or viable bacteria isolated by culturing, on the plant parts such as roots, stems, leaves, seeds, and the like, or in the culture soil, outbreaks of various plant diseases in an extremely wide range can be controlled, and pests can also be controlled. In addition, the plants on which the agents for controlling plant diseases and the agents for controlling pests comprising the D747 strain according to the present invention have been sprayed can exhibit superior controlling effects with respect to various plant diseases and pests.

Description

technical field [0001] The present invention relates to bacillus (Bacillus) D747 bacterial strain and relevant application thereof, specifically, relate to the plant disease controlling agent and the / or pest control agent, and the control method using the above-mentioned control agent. Background technique [0002] As methods for preventing and controlling plant diseases and pests, there are, for example, crop rotation, solar heat for cultivation or physical control, chemical control with chemical pesticides, control with disease-resistant varieties, and use of microorganisms that have an antagonistic effect on attenuated viruses or pathogenic bacteria. biological control. Among them, the development and research of chemical pesticides, especially organic synthetic fungicides, has developed extremely rapidly in recent years, and a variety of agents with high efficacy and different effects have been developed one after another, and various application methods have also been...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/22C12N1/20
CPCC12N1/20A01N63/00C12R1/07A01N63/22C12R2001/07C12N1/205
Inventor 渡边哲丰岛淳清水力山路孝二永山孝三矢野祐幸
Owner KUMIAI CHEM IND CO LTD
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