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Method for diagnostic acute myocardial infarction using serum specific protein group finger print atlas

An acute myocardial infarction and proteome technology, applied in the field of bioengineering, can solve the problems of identification, difficult skeletal muscle diseases, poor specificity, etc., and achieve the effects of improving the detection rate, prolonging the survival period, and low cost

Inactive Publication Date: 2005-03-02
高春芳
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  • Abstract
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  • Claims
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Problems solved by technology

Its disadvantages are: ① Poor specificity, especially difficult to differentiate from skeletal muscle diseases and injuries

Method used

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  • Method for diagnostic acute myocardial infarction using serum specific protein group finger print atlas
  • Method for diagnostic acute myocardial infarction using serum specific protein group finger print atlas

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Embodiment Construction

[0069] Using serum-specific proteomic fingerprints to diagnose acute myocardial infarction, the proteomic fingerprints were prepared first, and a certain amount of electrocardiograms, myocardial enzymes, and peripheral blood from patients with acute myocardial infarction, patients with unstable angina pectoris and normal controls confirmed by medical history were collected. After refrigerating at 4°C to precipitate the serum, centrifuge to absorb the serum, aliquot it, and store it at -80°C for later use. Dilute at a certain concentration during detection; use a metal chip; put it into a flying mass spectrometer from the American company Safeji to analyze the serum proteome spectrum of the sample; compare and analyze the serum proteome spectrum of patients with acute myocardial infarction, patients with unstable angina pectoris and healthy people, and obtain Specific protein peaks in the serum of patients with acute myocardial infarction; combine the specific protein peaks foun...

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Abstract

The invention relates to a method for diagnosing acute myocardial infarction the with specific protein fingerprint maps. First, making the protein form. taking specific quantity of the peripheroblood which diagnosed to be acute myocardial infarction and unstable angino, after centrifugally absorbing the blood serum, refrigerating at 4deg.C and separating the blood serum, packing separately, preparing in minus 80 deg.C, diluting at a specific thickness when detecting; adopting the metal chip made by American Fujiny corporation and put it into the fly mass spectrograph of the Fujiny corporation,analysing the serum protein group wave spectrum, comparing and analysing the serum protein wave spectrum of the acute myocardial infarction and unstable angino patient and the healthy man to acquire the serum specific protein wave peak of the acute myocardial infarction patient; combining the discovered serum protein wave peak to form a protein form, adopting the double blind study of the form to make clinical screening test to determine the positive forecast value, negtive forecast value, sensitivity and specificity.

Description

Technical field: [0001] The invention relates to the field of bioengineering, and relates to a method for diagnosing acute myocardial infarction by using serum-specific proteome fingerprints. Background technique: [0002] With the completion of most of the sequencing work of the human genome, the gene framework has been drawn. However, the function of the gene is not only determined by its own sequence, but also gives life a lot of information and wide variation, making the disease mechanism an extremely important issue. However, the ultimate executor of gene function is protein. Therefore, studying the structure and properties of protein is the most critical link in revealing the chain of genes and diseases. [0003] In the past, the research on protein mostly used analytical chemistry research techniques such as chromatographic separation and purification, two-dimensional electrophoresis, spectroscopy, and mass spectrometry. The necessary technical conditions for researc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/04G01N30/72G01N30/86G01N30/88
Inventor 高春芳赵光宋国英
Owner 高春芳
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