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Manufacturing method of tissue engineered cartilage

A technology of tissue engineering and manufacturing method, which is applied in the direction of biochemical equipment and methods, tissue culture, bone/connective tissue cells, etc. It can solve the problems of restricting the development and application of cartilage tissue engineering products, easy deformation, etc., so that there will be no immunity Good repellency, reasonable microstructure and good biocompatibility

Inactive Publication Date: 2005-01-19
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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Problems solved by technology

However, when the polymer degrades in the body, it will cause the accumulation of acidic products, which will have an adverse effect on the regeneration of cartilage tissue; collagen or gel materials will easily deform when exposed to water, which is not enough to provide continuous and strong support for the regeneration of chondrocytes
The above two deficiencies seriously limit the development and application of cartilage tissue engineering products.

Method used

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  • Manufacturing method of tissue engineered cartilage
  • Manufacturing method of tissue engineered cartilage
  • Manufacturing method of tissue engineered cartilage

Examples

Experimental program
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Embodiment Construction

[0016] The repair of embodiment sheep autologous articular cartilage defect

[0017] 1) Construction of tissue engineered cartilage

[0018] All steps are performed with reference to relevant 3DA standards. Twenty-eight Chinese Merino sheep (6 months old, male or female) were randomly divided into three groups: the experimental group (10), the material control group (10), and the blank control group (4). Cartilage tissue, about 3-5 g, was taken from the non-weight-bearing area of ​​the articular surface. The cells were digested overnight at 4° C. in 0.5% type II collagenase, and the cells were collected and cultured in a 75 ml plastic culture bottle. The medium was DMEM+10% fetal calf serum (FCS), 0.25% trypsin. Collect the 3rd passage cells and make 1.5~2×10 7 / ml of cell suspension, inoculated onto a cylindrical (diameter 10mm, thickness 4mm) α-TCP support, try to make the cells evenly distributed. The α-TCP porous bioceramic material is produced by the patented method a...

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Abstract

The invention belongs to tissue engineering field, and discloses a manufacturing method of tissue engineered cartilage comprising needed degradable porous biological ceramic materials. The invention composite generates cartilage tissue by inorganic biological bracket materials(alpha tricalcium phosphate porous ceramic materials) and tissue cell(cartilage cell) to restore articular cartilage injury. The invention can realize functional restoration of cartilage injury and has a considerate clinical practice prospect.

Description

field of invention [0001] The invention relates to a method for manufacturing tissue engineered cartilage, including the required degradable porous bioceramic support material, belonging to the field of tissue engineering. Background technique [0002] Articular cartilage trauma repair is still a great challenge in clinical medicine. Current treatment options include cartilage or bone-cartilage grafts and artificial joints. Autologous cartilage is the best transplant material, but it is not ideal in terms of application because of the limited body size and the need for surgery at a second site to obtain sufficient cartilage tissue for transplantation. However, the replacement repair of artificial joints has so far been difficult to achieve functional repair effects, and is often accompanied by various complications. Despite many years of unremitting efforts in this field, there is still a need to find an effective treatment for the repair of articular cartilage damage, pro...

Claims

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Application Information

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IPC IPC(8): A61L27/00A61L27/40A61L27/50C12N5/077
Inventor 郭希民王常勇段翠密赵强
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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